Due to local overactive inflammatory response and impaired angiogenesis, current treatments for diabetic wounds remain unsatisfactory. M2 macrophage-derived exosomes (MEs) have shown considerable potential in biomedical applications, especially since they anti-inflammatory properties that modulate macrophage phenotypes. However, exosome-based strategies still limitations, such as short half-lives instability. Herein, we develop a double-layer microneedle-based wound dressing system (MEs@PMN)...

Osteoporosis bone defect is a refractory orthopaedic disease which characterized by impaired quality and regeneration capacity. Current therapies, including antiosteoporosis drugs artificial grafts, are not always satisfactory. Herein, strontium-substituted calcium phosphate silicate bioactive ceramic (Sr-CPS) was fabricated. In the present study, extracts of Sr-CPS were prepared for in vitro study scaffolds used vivo study. The cytocompatibility, osteogenic osteoclastogenic properties...

Abstract Spinal cord injury (SCI) is one of the most common causes death and disability. The effective modulation complicated microenvironment, regeneration injured spinal tissue, functional recovery after SCI are still clinical challenges. Recently, macrophages‐derived exosomes have shown great potential for various diseases due to their inflammation‐targeting property. However, further modifications needed endow with neural regenerative recovery. In current study, a novel nanoagent (MEXI)...

Abstract Spinal cord injury (SCI) is a severe neurological disorder characterized by significant disability and limited treatment options. Mitigating the secondary inflammatory response following initial primary focus of current research in SCI. CCL2 (C─C motif chemokine ligand 2) serves as regulator responsible for chemotaxis majority peripheral immune cells, blocking CCL2‐CCR2 receptor type axis has shown considerable therapeutic potential diseases, including In this study, it presents...

Skin wounds accompanied by bacterial infections threaten human health, and conventional antibiotic treatments are ineffective for drug-resistant chronically infected wounds. The development of non-antibiotic-dependent therapeutics is highly desired but remains a challenging issue. Recently, 2D silicene nanosheets with considerable biocompatibility, biodegradability, photothermal-conversion performance have received increasing attention in biomedical fields. Herein, copper-containing...

Ossification of the posterior longitudinal ligament (OPLL) is a disabling disease whose pathogenesis still unclear, and there are no effective cures or prevention methods. Exosomal miRNA plays an important role in osteogenesis ectopic bone. Therefore, we focused on downregulation miR-140-5p OPLL cell-derived exosomes to explore mechanism by which exosomal inhibits OPLL.Exosomes were isolated differential centrifugation identified transmission electron microscopy, nanoparticle tracking...

Dental pulp regeneration is significantly aided by human dental stem cells (hDPSCs). An increasing number of studies have demonstrated that circular RNAs (circRNAs) are crucial in the multidirectional differentiation many mesenchymal cells, but their specific functions and mechanisms remain unknown. This work aimed at elucidating molecular mechanism which hsa_circ_0001599 works hDPSCs during odontogenic differentiation. The expression tissue was determined using quantitative real-time PCR...

Abstract Aim Dental pulp stem cells (DPSCs) are essential for regeneration but face low survival rates after transplantation. Genetic modification before transplantation is a promising solution to this issue. We aim elucidate the biological function and regulatory mechanism of hypoxic lncRNA HRL‐SC in DPSCs. Methodology The functions hypoxia inducible factor‐1α (HIF‐1α) DPSCs were evaluated vitro by cell proliferation, migration tube formation assays. Subcutaneous nude mice was used evaluate...

Wear particles that detach from the surface of prostheses induce excessive activation osteoclast and immoderate release inflammatory cytokines lead to peri-implant osteolysis aseptic loosening. In this work, we investigated whether magnoflorine, a quaternary aporphine alkaloid extracted Chinese herb Magnolia or Aristolochia, could effectively inhibit calvarial caused by titanium in mouse models, response as well osteoclastogenesis vitro mediated via receptor activator NF-κB ligand (RANKL)....

Abstract Background Pulpitis is an inflammatory disease, the grade of which classified according to level inflammation. Traditional methods evaluating status dental pulp tissue in clinical practice have limitations. The rapid and accurate diagnosis pulpitis essential for determining appropriate treatment. By integrating different datasets from Gene Expression Omnibus (GEO) database, we analysed a merged expression matrix pulpitis, aiming identify biological pathways diagnostic biomarkers...

Abstract Background To study the protective effects of delayed remote ischemic preconditioning (RIPC) against spinal cord ischemia–reperfusion injury (SCIRI) in mice and determine whether SIRT3 is involved this protection portrayed its upstream regulatory mechanisms. Methods In vivo , WT or global knockout (KO) were exposed to right upper lower limbs RIPC sham ischemia. After 24 h, abdominal aorta was clamped for 20 min, then re-perfused 3 days. The motor function mice, number Nissl bodies,...

Introduction . Odontogenic differentiation of human dental pulp stem cells (hDPSCs) is a key step regeneration. Recent studies showed that circular RNAs (circRNAs) have many biological functions and competing endogenous RNA (ceRNA) their most common mechanism action. However, the role circRNAs in hDPSCs during odontogenesis still unclear. Methods Isolated were cultured essential odontogenic medium. Total was extracted after 14 days culture, then, microarray analysis performed to measure...

Ossification of the posterior longitudinal ligament (OPLL), an emerging heterotopic ossification disease, causes spinal cord compression, resulting in motor and sensory dysfunction. The etiology OPLL remains unclear but may involve integrin αVβ3 regulating process osteogenesis angiogenesis. In this study, we focused on role explored underlying mechanism by which c(RGDyk) peptide acts as a potent selective inhibitor to inhibit angiogenesis OPLL.

Human dental pulp stem cells (hDPSCs) are critical for generation. hDPSCs proliferate faster under hypoxia, but the mechanism by which long noncoding RNA (lncRNA) regulates this process is not fully understood.Novel lncRNAs were obtained reanalysis of transcriptome datasets from RNA-Seq hypoxia compared with normoxia, and a differential expression analysis target genes was performed. Bioinformatics analyses, including gene ontology analysis, Kyoto Encyclopedia Genes Genomes pathway set...

Abstract Background Cone-beam computed tomography (CBCT) was employed to study the morphology and curvature of middle mesial canals (MMCs) mandibular first molars (MFMs). Methods CBCT scanning performed on MFMs 1100 patients. Patients' images that met inclusion criteria were divided into group A (< 40 years old) B (≥ for further study. The used incidence MMCs at different ages, measure in mesiodistal buccolingual directions using Schneider method, observe anatomical root canal system. All...

Cone-beam computed tomography (CBCT) was used to study the root canal system of mandibular anteriors (MAs) in a Cantonese population and evaluate correlation between complicated configurations lateral incisors (MLIs) presence distolingual roots (DLRs) first molars (MFMs).A total 11,376 anterior teeth were scanned by CBCT. Those whose images met inclusion criteria analysed according Vertucci's configuration then grouped based on gender, age, side, their effects morphology analysed. Finally,...

Background: Human dental pulp stem cells (hDPSCs) play an important role in endodontic regeneration. N6-methyladenosine (m6A) is the most common RNA modification, and noncoding RNAs have also been demonstrated to regulatory roles expression of m6A proteins. However, study on modification hDPSCs has not yet conducted. Methods: Single base site PCR (MazF) was used detect lncSNHG7 before after mineralization screen target protein, bioinformatics analysis analyze related pathways rich lncSNHG7....

The purpose of this study was to evaluate the relationship between nerve root lower lumbar and surrounding structures using three-dimensional computed tomography (3D CT). Twenty-three consecutive patients with thoracolumbar fractures without obvious radiological degeneration were retrospectively studied at spinal surgery department hospital. parameters measured 3D CT in work station picture archiving communication system. size dorsal ganglion (DRG) L4 5.5 ± 0.4 mm on right side 5.8 0.3 left...