A5078948402- Crustacean biology and ecology
- Alkaline Phosphatase Research Studies
- Aquaculture Nutrition and Growth
- Physiological and biochemical adaptations
- Bone health and treatments
- Parathyroid Disorders and Treatments
- Neurobiology and Insect Physiology Research
- Folate and B Vitamins Research
- Biochemical and Molecular Research
- Bone health and osteoporosis research
- Fish biology, ecology, and behavior
- Enzyme function and inhibition
- Enzyme Production and Characterization
- Ion Transport and Channel Regulation
- Environmental Toxicology and Ecotoxicology
- ATP Synthase and ATPases Research
- Enzyme Catalysis and Immobilization
- Vitamin D Research Studies
- Selenium in Biological Systems
- Erythrocyte Function and Pathophysiology
- Calcium Carbonate Crystallization and Inhibition
- Electrochemical sensors and biosensors
- Lipid Membrane Structure and Behavior
- thermodynamics and calorimetric analyses
- Mitochondrial Function and Pathology
Universidade de Ribeirão Preto
2012-2024
Universidade de São Paulo
2015-2024
SUPERA Park of Innovation and Technology of Ribeirão Preto
1990-2015
In-Q-Tel
2010
University of Milan
2009
Seevic College
1990-2006
Universidad Nacional de Córdoba
2005
Research Centre in Biological Chemistry of Córdoba
2005
Consejo Nacional de Investigaciones Científicas y Técnicas
2005
Université Joseph Fourier
1998-1999
Abstract SigrafW is Windows‐compatible software developed using the Microsoft® Visual Basic Studio program that uses simplified Hill equation for fitting kinetic data from allosteric and Michaelian enzymes. a modified Fibonacci search to calculate maximal velocity ( V ), coefficient n enzyme‐substrate apparent dissociation constant K ). The estimation of , sum squares residuals performed Wilkinson nonlinear regression at any In contrast many currently available analysis programs, shows...
A new biotin derivative functionalized by an electropolymerizable pyrrole group has been synthesized. The electrooxidation of this allowed the formation biotinylated conducting polypyrrole films in organic electrolyte. Gravimetric measurements based on a quartz crystal microbalance, modified polymer, revealed avidin−biotin-specific binding at interface polymer−solution. estimated mass increase corresponded to anchoring 1.5 avidin monolayers surface. In addition, subsequent grafting glucose...
A mycelial β-glucosidase from the thermophilic mold Humicola insolens was purified and biochemically characterized. The enzyme showed carbohydrate content of 21% apparent molecular mass 94 kDa, as estimated by gel filtration. Sodium dodecyl sulfate polyacrylamide electrophoresis analysis a single polypeptide band 55 suggesting that native homodimer. Mass spectrometry amino acid sequence similarity with grisea var. thermoidea, about 22% coverage. Optima temperature pH were 60 °C 6.0–6.5,...
Phosphodiesterase activity is a novel property of the still-enigmatic alkaline phosphatase from osseous plate. Bis-(p-nitrophenyl) phosphate was hydrolysed at both pH 7.5 and 9.4 with an apparent dissociation constant (K0.5) 1.9 mM 3.9 respectively. The hydrolysis p-nitrophenyl-5'-thymidine followed hyberbolic kinetics K0.5 500 microM. For p-nitrophenyl phenylphosphonate, site-site interactions [Hill coefficient (h) = 1.3] were observed in range between 0.2 100 microM, 32.8 mM. cyclic AMP by...
The electropolymerization of an alkaline phosphatase (AP)−amphiphilic pyrrole ammonium mixture previously adsorbed on the electrode surface provides efficient entrapment AP in a polypyrrole film. resulting biosensor was applied to detection phenyl phosphate via amperometric oxidation at 0.6 V vs SCE enzymically generated phenol. sensitivity and limit were 862 μA M-1 cm-2 20 μM, respectively. co-immobilization polyphenol oxidase leads bienzyme for determination basis o-quinone −0.2 V. An...
The catalytic activity of a glycosylphosphatidylinositol (GPI)-anchored alkaline phosphatase has been studied in Langmuir phospholipid monolayers at different surface pressures. enzyme substrate, p-nitrophenyl phosphate, was injected into the subphase mixed enzyme/lipid monolayers. Its hydrolysis product followed by monitoring absorbance 410 nm situ monolayer trough. Several pressures, corresponding to molecular densities, were attained lateral compression morphology monolayers, observed...
The effect of NH4+ ions on (Na+,K+)-ATPase hydrolytic activity was examined in a gill microsomal fraction from M. olfersii. In the absence ions, K+ stimulated ATP hydrolysis, exhibiting cooperative kinetics (nH=0.8), to maximal specific V=556.1+/-22.2 nmol.min(-1).mg(-1) with K(0.5)=2.4+/-0.1 mmol.L(-1). No further stimulation by observed presence 50 mmol.L(-1) ions. hydrolysis also obeying Michaelian V=744.8+/-22.3 and KM=8.4+/-0.2 10 synergistically V=689.8+/-13.8 K(0.5)=6.6+/-0.1...