A5045064177- PARP inhibition in cancer therapy
- Toxin Mechanisms and Immunotoxins
- DNA Repair Mechanisms
- Calcium signaling and nucleotide metabolism
- Protein Interaction Studies and Fluorescence Analysis
- Mass Spectrometry Techniques and Applications
- Drug Transport and Resistance Mechanisms
- Connexins and lens biology
- Genomics, phytochemicals, and oxidative stress
- Wound Healing and Treatments
- Metabolism and Genetic Disorders
- Folate and B Vitamins Research
- Muscle metabolism and nutrition
- Diet, Metabolism, and Disease
- Skin Protection and Aging
University of Zurich
2013-2018
Life Science Zurich
2017-2018
ETH Zurich
2013-2016
Institute of Molecular Genetics
2013
Abstract ADP-ribosylation is a posttranslational modification that exists in monomeric and polymeric forms. Whereas the writers (e.g. ARTD1/PARP1) erasers PARG, ARH3) of poly-ADP-ribosylation (PARylation) are relatively well described, enzymes involved mono-ADP-ribosylation (MARylation) have been less investigated. While for MARylation glutamate/aspartate arginine identified, respective with specificity serine were missing. Here we report that, vitro, ARH3 specifically binds demodifies...
Creatine transport has been assigned to creatine transporter 1 (CRT1), encoded by mental retardation associated SLC6A8. Here, we identified a second (CRT2) known as monocarboxylate 12 (MCT12), the cataract and glucosuria gene SLC16A12. A non-synonymous alteration in MCT12 (p.G407S) found patient with age-related (ARC) leads significant reduction of transport. Furthermore, Slc16a12 knockout (KO) rats have elevated levels urine. Transport activity expression characteristics two transporters...
Oxidative stress is a potent inducer of protein ADP-ribosylation. Although individual oxidative stress-induced ADP-ribosylated proteins have been identified, it so far not clear to which extent different degrees severity quantitatively and qualitatively alter Here, we investigated both quantitative qualitative changes the hydrogen peroxide (H2O2)-induced ADP-ribosylome using label-free shotgun quantification parallel reaction monitoring (PRM) mass spectrometry approach for selected number...