We report a manganese-catalyzed aliphatic C–H azidation reaction that can efficiently convert secondary, tertiary, and benzylic bonds to the corresponding azides. The method utilizes aqueous sodium azide solution as source be performed under air. Besides its operational simplicity, potential of this for late-stage functionalization has been demonstrated by successful various bioactive molecules with yields up 74%, including important drugs pregabalin, memantine, antimalarial artemisinin....

Serum levels of the tumor marker CA 19–9 are widely utilized in diagnosis and monitoring pancreatic biliary malignancies. However, serum have also been reportedly elevated non-malignant conditions. Here, we present rare case a 65-year-old woman with history gallbladder malignancy who was found to new hepatic lesion on surveillance CT an associated elevation 5,866 U/mL. Drainage treatment antibiotics resulted rapid decline levels, indicating that due benign lesion. We review eight similar...

Abstract Ovarian cancer is the sixth leading cause of cancer-related mortality among individuals with ovaries, and high-grade serous ovarian (HGSOC) most common lethal subtype. Characterized by a distinct aggressive metastatic pattern, HGSOC can originate in fallopian tube transformation epithelial (FTE) cells, which metastasize to ovary subsequently omentum peritoneal cavity. The privileged site, metabolic exchange underlying omental metastasis could provide enzyme or receptor targets block...

<p>Figure S7. LC-MS data from amino acids quantified using aTRAQ kit (Sciex).</p>

<p>Figure S5. Signals from Figure S4 that replicated when cells and omentum are physically separated in agarose.</p>

<p>Figure S8. Proliferation and mTOR phosphorylation with supplementation to 2.4mM valine.</p>

<p>Putative annotations and parts per million error</p>

<p>LC/MS data show increased consumption of BCAAs and serine no change in histidine concentration under co-culture with omentum (OM). LC/MS results from the analysis 3D agarose coculture extracts using aTRAQ kit to determine concentrations (<b>A</b>) valine, (<b>B</b>) leucine, (<b>C</b>) isoleucine, (<b>D</b>) serine, (<b>E</b>) resuspended at 1 mg/mL. Significance was determined one-way ANOVA Tukey <i>post...

<p>MSI analysis shows several signals specific to the tumorigenic FTE cell/omentum coculture condition. <b>A,</b> Illustration of imaging layout on slide for untargeted analysis, including cells (MOE PTEN<sup>shRNA</sup>) and nontumorigenic controls. <b>B,</b> Ion images showing signal intensity four representative upregulated in HGSOC condition PTEN mutation (<i>N</i> = 3). <b>C,</b> divided well identify origin signals....

<p>Valine supplementation increases the proliferation of tumorigenic FTE cells and activates mTOR signaling. <b>A,</b> Schematic activation by BCAAs. <b>B–E,</b> Sulforhodamine B data from MOE SCR<sup>shRNA</sup>, MOSE, PTEN<sup>shRNA</sup>, PTEN<sup>shRNA</sup> p53<sup>R273H</sup> in DMEM, 1.6 mmol/L valine 1 μmol/L rapamycin (RAP), or combination. Significance was determined using one-way ANOVA with Tukey’s...

<div>Abstract<p>Ovarian cancer is the sixth leading cause of cancer-related mortality among individuals with ovaries, and high-grade serous ovarian (HGSOC) most common lethal subtype. Characterized by a distinct aggressive metastatic pattern, HGSOC can originate in fallopian tube transformation epithelial (FTE) cells, which metastasize to ovary subsequently omentum peritoneal cavity. The privileged site, metabolic exchange underlying omental metastasis could provide enzyme or...

<p>Figure S1. Omentum coculture drying photo.</p>

<p>Figure S2. Live dead staining of omental tissue</p>

<p>Ion suppression tests for select amino acids in cell culture media. <b>A,</b> Ion images showing signal intensity of <i>m/z</i> 118.09 with different concentrations (Conc.) a valine analytic standard spiked into agarose. Standard curve 1 shows ranging from 800 to 0.08 μmol/L, whereas 2 20 1.25 μmol/L. DMEM contains μmol/L L-valine. <b>B,</b> 156.07 histidine 200 0.05 0.2 L-histidine.</p>

<p>Figure S4. Other signals identified in the initial MSI screen</p>

<p>Figure S6. MALDI-MS/MS fragmentation data from tumorigenic FTE/omentum coculture extract reveals the signal at m/z 118 represents L-valine.</p>

<p>Figure S3. Omentum IMS replicates</p>

High-grade serous ovarian cancer (HGSOC) can originate in the fallopian tube and then spread to ovary. Our objective was evaluate role of multicellular tumor spheroids (MTS) metastasis. By testing a panel murine oviductal epithelial (MOE) cells with genetic alterations mimicking those seen HGSOC, we found that loss PTEN allowed MTS formation under ultra-low adhesion conditions. Confirming these results vivo, MTS-like structures were observed oviducts PAX8Cre/+ PTENflox/flox mice. MOE...

Progesterone functions as a steroid hormone involved in female reproductive physiology. While some disorders manifest with symptoms that can be treated by progesterone or synthetic progestins, recent data suggest women also seek botanical supplements to alleviate these symptoms. However, are not regulated the U.S. Food and Drug Administration therefore it is important characterize quantify inherent active compounds biological targets of within cellular animal systems. In this study, we...

High-grade serous ovarian cancer is the most common and lethal gynecologic malignancy, which often attributed to lack of available screenings, allowing disease progress unnoticed until it diagnosed at more aggressive stages. As such, identifying signals in tumor microenvironment involved primary metastasis tumorigenic fallopian tube epithelial (FTE) cells ovary could provide new avenues for prevention, diagnostics, or therapeutic intervention. Since our previous work identified that...

High grade serous ovarian cancer (HGSOC), the most lethal histotype of cancer, frequently arises from fallopian tube epithelial cells (FTE). Once transformed, tumorigenic FTE often migrate specifically to ovary, completing crucial primary metastatic step and allowing formation tumors after which HGSOC was originally named. As only fimbriated distal ends that reside in close proximity ovary develop precursor lesions such as tubal intraepithelial carcinomas, this suggests process...