Prostaglandin (PG) I(2) and thromboxane (TX) A(2), the most common prostanoids in cardiovascular system, are produced abundantly during cardiac ischemia/reperfusion (I/R); their roles I/R injury, however, remain undetermined. We intended to clarify these of PGI(2) TXA(2) using mice lacking receptor, IP(-/-) mice, or TP(-/-) mice.The left anterior descending coronary artery was occluded for 1 hour then reperfused 24 hours. The size myocardial infarct significantly larger than that wild-type...

In the heart with acute myocardial infarction, production of prostaglandin (PG) E2 increases significantly. addition, several subtypes PGE2 receptors (EPs) have been reported to be expressed in heart. The role cardiac ischemia-reperfusion (I/R) injury, however, remains unknown. We intended clarify via EP4, an EP subtype, I/R injury using mice lacking EP4 (EP4-/- mice).In murine ventricle, competitive reverse transcription-polymerase chain reaction revealed highest expression level mRNA among...

Persistent reduction of renal perfusion pressure induces renovascular hypertension by activating the renin-angiotensin-aldosterone system; however, sensing mechanism remains elusive. Here we investigated role PGI2 in vivo, employing mice lacking receptor (IP-/- mice). In WT with a two-kidney, one-clip model hypertension, BP was significantly elevated. The increase IP-/- mice, lower than that mice. Similarly, increases plasma renin activity, mRNA, and aldosterone response to artery stenosis...

In the heart, expressions of several types prostanoid receptors have been reported. However, their roles in cardiac hypertrophy vivo remain unknown. We intended to clarify these pressure overload-induced using mice lacking each receptors.We used a model produced by banding transverse aorta female mice. wild-type subjected banding, developed during observation period 8 weeks. prostaglandin (PG) I2 receptor (IP(-/-)), however, and cardiomyocyte were significantly greater than at 2 4 weeks but...

Endothelial progenitor cells (EPCs) play an important role in the self-healing of a vascular injury by participating reendothelialization that limits remodeling. We evaluated whether prostaglandin I(2) plays regulation function EPCs to limit remodeling.EPCs (Lin(-)cKit(+)Flk-1(+) cells) were isolated from bone marrow (BM) wild-type (WT) mice or lacking receptor IP (IP(-/-) mice). Reverse transcription-polymerase chain reaction analysis showed among BM specifically express IP. The cellular...

<i>Background:</i> The production of reactive oxygen species (ROS) has been suggested to play an important role in the progression chronic kidney disease (CKD). An oral adsorbent, AST-120, removes uremic toxins such as indoxyl sulfate (IS) and delays CKD, but effect on ROS is unknown. present study aimed determine whether AST-120 reduces oxidative stress rat kidneys using markers acrolein 8-hydroxy-2′-deoxyguanosine (8-OHdG). <i>Methods:</i> Daily administration was...

Minimal change nephrotic syndrome (MCNS) is a common form of (NS). We herein present the case 57-year-old woman with advanced lung adenocarcinoma treated tyrosine kinase inhibitor (TKI) gefitinib who developed NS. A renal biopsy revealed minor glomerular abnormalities, and patient's symptoms improved exclusively discontinuation gefitinib. Therefore, we diagnosed her MCNS associated treatment. few months later, however, she recurrent tumors. Following challenging initiation TKI erlotinib,...

Prevalent atrial fibrillation (AF) in patients with hypertrophic cardiomyopathy (HCM) represents an important issue regard to stroke events caused by embolization and is associated high mortality. Increased epicardial adipose tissue (EAT), which shows metabolic activity, can locally influence the activity of autonomic ganglia, enhancing dysregulation increasing likelihood AF. We tested hypothesis that EAT prevalent AF HCM patients. Sixty-two idiopathic diagnosed on basis ultrasound...

In response to oxidative stress, the pathogenesis of a number cardiovascular events and several genes are stimulated by extracellular signal-regulated kinases (ERK1/2). Biphasic (early, 10 min; delayed, 120 min) ERK1/2 activation H(2)O(2), reactive oxygen species, was observed in cultured neonatal rat cardiomyocytes. We investigated hypothesis that delayed depends on factor secreted stress (FSO). The inhibited calphostin C, protein kinase C inhibitor. Conditioned medium (CM) obtained from...

Circulatory failure in sepsis arises from vascular hyporesponsiveness, which nitric oxide (NO) derived inducible NO synthase (iNOS) plays a major role. Details of the cross talk between thromboxane (TX) A2 and iNOS-NO system, however, remain unknown. We intended to clarify role TXA2, via talk, hyporesponsiveness.We examined cytokine-induced iNOS expression production cultured smooth muscle cells (VSMCs) hyporesponsiveness aorta mice lacking TXA2 receptor (TP-/- mice). The observed wild-type...

p53, a pivotal protein in the apoptotic pathway, has been identified as mediator of transcriptional responses to ischemia-reperfusion (IR) injury. The characteristics and functional significance p53 response vivo are largely unknown IR-induced kidney Therapeutic opportunities delivering small interfering RNA (siRNA) via venous injection have gained recognition; however, systemic adverse effects siRNA therapy should be considered. To prevent injury, we tested efficacy transarterial...

Although heparin-binding epidermal growth factor-like factor (HB-EGF) is thought to produce hypertrophy in isolated cardiomyocytes via an autocrine mechanism, the pathophysiological role of HB-EGF, myocardial vivo, not yet known. To investigate involvement HB-EGF cardiac remodeling associated with hypertension we assayed expression mRNA and protein left ventricle (LV) during development ventricular spontaneously hypertensive rats (SHR).Prior sacrifice assay EGF-receptor (EGF-R) mRNA,...

Reactive oxygen species (ROS) enhance myocardial ischemia-reperfusion (I/R) injury. Ischemic preconditioning (PC) provides potent cardioprotective effects in I/R. However, it has not been elucidated whether PC diminishes ROS stress I/R and protects the myocardium from transmurally homogeneously. Isolated rabbit hearts perfused with Krebs-Henseleit buffer underwent 30 min of ischemia 60 reperfusion. Hemodynamic changes damage extent were analyzed four groups. The control group alone. H2O2...

Histone H3K4 trimethylation (H3K4 me3) is found in active euchromatic regions and plays an important role podocyte function which actin filaments are abundant the foot processes. The pathogenesis of membranous nephropathy (MN), most prevalent cause primary nephrotic syndrome middle-aged elderly, dysfunction. We investigated me3 dysfunction using cultured podocytes a mouse proteinuria model induced by LPS. examined renal biopsy specimens from six patients with caused...

Effects of the prostanoids on growth cultured aortic vascular smooth muscle cells (VSMCs) were examined using mice lacking prostanoid receptors. Proliferation VSMCs was assessed by measuring [ 3 H]‐thymidine incorporation and cell number, their hypertrophy 14 C]‐leucine protein content. In from wild‐type mice, expressions mRNAs for EP 4 TP most abundant, followed those IP, FP, when competitive reverse transcriptase‐PCR. Those 1 , 2 DP, however, could not be detected. AE1‐329, an agonist,...

We reported impaired QT-rate dependence in early repolarization syndrome (ERS); however, contemporary data have shown peak incidence of sudden cardiac death (SCD) ERS and Brugada (BrS) at mid-night morning. Taken together, we analyzed the nocturnal both syndromes.A total 172 subjects were enrolled: 11 ERS, BrS patients, 50 with an uneventful ER pattern (ERP), 100 non-J-wave control subjects. Ambulatory ECG-derived parameters (QT, QTc, QT/RR slope) day-night QT difference compared. Among...

Coexistence of horseshoe kidney and abdominal aortic aneurysm (AAA) is a rare entity that presents technical challenge to vascular surgeons. How approach such an AAA with whether divide the renal isthmus remains controversial issue. We report here successful surgical repair via transperitoneal division by Harmonic Focus, which allowed easy without bleeding. hand-held type harmonic scalpel, was thus useful in symphysiotomy kidney.

Systemic capillary leak syndrome (SCLS) is a life-threatening disorder which presents with periodic episodes of hypovolemic shock, due to plasma leakage the extra-vascular space reflected by accompanying hypoalbuminemia, hemoconcentration and edema often associated monoclonal gammopathy. We describe 28-year-old woman SCLS who required aggressive fluid resuscitation was successfully treated corticosteroid, terbutaline, theophylline. At exacerbation, levels serum granulocyte colony-stimulating...

Objective Tonsillectomy and steroid-pulse (TSP) therapy have been proposed as a curative treatment for immunoglobulin A nephropathy (IgAN) in Japan. However, we sometimes encounter patients who reject because of concerns about the side effects corticosteroids. Here, examined efficacy TSP tonsillectomy alone IgAN with urinary abnormalities. Methods Data on 40 diagnosed by renal biopsies, presented glomerular hematuria proteinuria at baseline underwent bilateral palatine tonsillectomy, were...