Leaf size is a major determinant of plant photosynthetic activity and biomass; however, it poorly understood how leaf genetically controlled in cereal crop plants like barley (Hordeum vulgare). We conducted genome-wide association scan for flowering time, width, length diverse panel European winter cultivars grown the field genotyped with single-nucleotide polymorphism array. The identified PHOTOPERIOD-H1 (Ppd-H1) as candidate gene underlying quantitative trait loci time population....

A collection of 379 Hordeum vulgare cultivars, comprising all combinations spring and winter growth habits with two six row ear type, was screened by genome wide association analysis to discover alleles controlling traits related grain yield. Genotypes were obtained at 6,810 segregating gene-based single nucleotide polymorphism (SNP) loci corresponding field trial data for eight yield four European sites in three countries over years. The combined analyzed statistically significant...

The Pik-h gene in rice confers resistance to several races of blast fungus (Magnaporthe oryzae), and has been classified as a member the Pik cluster, one most (R) gene-dense regions genome. However, loss key mutant isolate long made it difficult differentiate from other group genes especially Pik-m. We identified new natural isolates enabling differentiation between Pik-m genes, first confirmed authenticity International Rice Research Institute (IRRI) “monogenic” line IRBLkh-K3, then...

Field resistances (FR) against rice blast are highly evaluated by breeders for their durability, in contrast to the conspicuous but often less durable true resistances. However, lack of efficient systems evaluation resistance has delayed practical application. Kahei, an upland domestic cv., is known its very high FR blast. We fine-mapped highest quantitative trait loci (QTL), qBFR4-1, using residual heterozygosity recombinant inbred lines (RILs) and our semi-natural inoculation/evaluation...

Fatty acids (FAs) have been implicated in signaling roles plant defense responses. We previously reported that mutation or RNAi-knockdown (OsSSI2-kd) of the rice OsSSI2 gene, encoding a stearoyl acyl carrier protein FA desaturase (SACPD), remarkably enhanced resistance to blast fungus Magnaporthe oryzae and leaf-blight bacterium Xanthomonas pv. (Xoo). Transcriptomic analysis identified six AAA-ATPase family genes (hereafter OsAAA-ATPase1–6) upregulated OsSSI2-kd plants, addition other...

Triacylglycerol Lipases (TGLs) are the major enzymes involved in triacylglycerol catabolism. TGLs hydrolyze long-chain fatty acid triglycerides, which plant development and abiotic stress responses. Whereas most studies of have focused on seed oil metabolism biofuel plants, limited information is available regarding genome-wide identification characterization TGL gene family tomato (Solanum lycopersicum L.). Based latest published genome annotation ITAG4.0, 129 SlTGL genes were identified...

Rapid and inexpensive preparation of genomic DNA from rice seeds for marker-assisted selection seed purity estimation is a major bottleneck plant breeders. Here, we describe high-throughput method that provides at sufficient quantity quality these applications. Optimization buffer composition individual protocol stages allow processing 384 samples within 2 h, yielding templates reliably support downstream polymerase chain reaction single copy amplicons up to 1.2 kb.

Bulked segregant analysis (BSA) of a BC1 population derived from congguang 41A/MiYang 23//congguang 41B was used to map the nuclear fertility restorer gene Rf5 for HongLian (HL) cytoplasmic male sterility. The parents and two bulks representing extremely fertile sterile plants, respectively, were screened polymorphism with 20 microsatellite primer pairs on chromosome 10, chosen basis previous research. MRG4456 is linked at distance 1.57 cM, another newly developed primer, HL01, locked 0.63...

Diacylglycerol acyltransferase (DGAT) is a key enzyme that catalyzes the final and rate-limiting step of triglyceride synthesis.Both DGAT1 DGAT2 genes code proteins with DGAT activity.Studies have shown polymorphisms associate intramuscular fat deposition in beef cattle, but fewer associations between cattle economic traits been reported.The objective this study was to investigate single nucleotide polymorphism (SNP) intron3 bovine evaluate carcass, meat quality, yield traits.Test animals...

Rice blast resistance (R) genes-mediated response depends on various resistance-related genes involved in incompatible interactions. In this work, the expression profiles of innate rice immunity related were examined mediated true/field genes. Three sets near-isogenic lines (NILs) used: resistant NILs carrying true genetic background susceptible cultivar Nipponbare (NB), NB-Pib, NB-Pizt, NB-Pik and NB-Pita2; bearing field pi21 Aichiasahi (AA) AA-pi21, Kahei (KHR). The marker gene OsWRKY45...

The voltage-dependent anion channel (VDAC), also known as a mitochondrial porin, plays an important role in the regulation of metabolic and energetic functions mitochondria, well mitochondria-mediated apoptosis.Cytoplasmic male sterility (CMS) is major economic importance for commercial hybrid production research model interaction between nuclear cytoplasmic genomes.Recent has revealed that CMS associated with programmed cell death.Here, we used Honglian (HL)-CMS line rice (Oryza sativa)...

Background: Acidic polysaccharides usually possess favorable bioactivities, and some of them are applied in medicines dietary supplements together. However, it is a challenge to quantify these simultaneously. Methods: A method Quantify Hyaluronic Acid (HA), Chondroitin Sulfate (CS) nonglycosaminoglycan acidic polysaccharide named AGSP was established by determining the contents their disaccharides produced from acid hydrolysis using hydrophilic interaction chromatographytandem triple...

Three side-chain liquid crystalline ionomers (SLCIs) with sulfonic group (SLCI-S), quaternary ammonium salt (SLCI-N), and carboxylic (SLCI-C) were synthesized by graft copolymerization upon polymethylhydrosiloxane, respectively. The SLCIs used to blend poly (butylene terephthalate) (PBT) polypropylene (PP). morphological structure mechanical properties of the blends investigated scanning electron microscopy (SEM). SEM results indicated that addition lead finer better dispersion PP polymer in...

The main aim of the work is to study regulation gene expression in interaction between rice and Magnaporthe oryzae by chip technology. In this study, we mainly focused on changes at 24, 48, 72 hours post-inoculation (hpi), through which could conduct a more comprehensive analysis blast-related genes process infection. results showed that experimental groups contained 460, 1227, 3937 significant differentially expressed hpi, respectively. Furthermore, 115 significantly were identified...

Abstract The identification and development of new cytoplasmic male sterility (CMS) lines in higher plants is important for the preservation grain security prevention homogenization hybrid rice. Molecular markers assisted selection (MAS) based on CMS-associated genes or mitochondrial-specific chimeric sequences are rapid effective breeding CMS hybrids. In our study, distribution allele variation orfH79 orf290 were characterized from 273 wild cultivated rice AA genome species. Based alignment...