We measured the influences of soil fertility and plant community composition on Glomeromycota, tested prediction functional equilibrium hypothesis that increased availability resources will reduce abundance arbuscular mycorrhizal (AM) fungi. Communities plants AM fungi were in mixed roots Elymus nutans across an experimental fertilization gradient alpine meadow Tibetan Plateau. As predicted, reduced Glomeromycota as well species richness The response glomeromycotan was strongly linked to...

Abstract The cardiac pacemaking and conduction system sets maintains the rhythmic pumping action of heart. Previously, we have shown that peripheral cells network in chick (periarterial Purkinje fibers) are selected within a cardiomyogenic lineage this recruitment occurs as result paracrine cues from coronary arteries. At present, cellular derivation other elements specialized (e.g. nodes bundles central system) controversial, with some proposing evidence supports neurogenic others myogenic...

While a vast number of studies have addressed the prokaryotic diversity in permafrost, characterized by subzero temperatures, low water activity, and extremely rates nutrient metabolite transfer, fungal patterns received surprisingly limited attention. Here, community structure were investigated culture-dependent technique combined with cloning-restriction fragment length polymorphism (RFLP) analysis sediments 10-m-long permafrost core from Qinghai-Tibet Plateau China. A total 62 phylotypes...

Understanding the processes that influence structure of biotic communities is one major ecological topics, and both stochastic deterministic are expected to be at work simultaneously in most communities. Here, we investigated vertical distribution patterns bacterial a 10-m-long soil core taken within permafrost Qinghai-Tibet Plateau. To get better understanding forces govern these patterns, examined diversity communities, change community composition along distance (spatial turnover) from...

Plants have evolved different abilities to adapt the ever-fluctuating environments for sessility. Calcium-dependent protein kinase (CDPK) is believed play a pivotal role in abiotic stress signaling. So far, study on specific substrates that CDPK recognized response adversity limited. In present study, we revealed potential interaction between and bHLH transcription factor under salt Chenopodium glaucum. First, identified CgCDPK, which was up-regulated drought stress; then by Y2H screening,...

Abstract To investigate spatial and temporal distributions of apoptosis in the embryonic chick heart its relation to different tissue types, we examined from Hamburger Hamilton stage 17 through 3 days after hatching. MF20 antibody, alpha‐smooth muscle actin (SMA) antibody EAP‐300 were applied delineate specific cell types. During early development heart, very few apoptotic cells detected. The first distinctive zone was observed outflow tract at 25. This focus most prominent during septation...

A novel Gram-staining-negative bacterial strain, designated XH4(T), was isolated from soil of a Populus euphratica forest in the Hotan River valley, Xinjiang Uyghur autonomous region, PR China. The cells were strictly aerobic, non-motile, short rods. isolate grew optimally at 37 °C and pH 7.0-8.0. Based on 16S rRNA gene sequence analysis, strain XH4(T) belonged to genus Sphingobacterium closely related mizutaii ATCC 33299(T) (96.1 % similarity). DNA G+C content 41.2 mol%. major polar lipid...

Fatty liver or hepatic steatosis is a common health problem associated with abnormal function and increased susceptibility to ischemia/reperfusion injury. The objective of this study was investigate the effect fatty acid synthase inhibitor cerulenin on in steatotic ob/ob mice. Different dosages were administered intraperitoneally mice for 2 7 days. Body weight, serum AST/ALT, energy state, gene expression patterns examined. We found that treatment markedly improved Serum AST/ALT levels...

Phosphoenolpyruvate carboxylase (PEPC) plays pivotal roles in the carbon fixation of photosynthesis and a variety metabolic stress pathways. Suaeda aralocaspica belongs to single-cellular C 4 species carries out photosynthetic pathway an unusually elongated chlorenchyma cell, which is expected have PEPCs with different characteristics. To identify isoforms PEPC genes S. comparatively analyze their expression regulation patterns as well biochemical enzymatic properties this study, we...

The heartbeat is initiated and coordinated by a multi-component set of specialized muscle tissues collectively referred to as the pacemaking conduction system. Over last few years, impetus has gathered into unravelling cellular molecular processes that regulate differentiation integration this essential cardiac network. One focus our collective work been developmental history cells comprising His-Purkinje This interest in part arose from studies expression connexins periarterial Purkinje...

Both deterministic and stochastic processes are expected to drive the assemblages of arbuscular mycorrhizal (AM) fungi, but little is known about relative importance these during spreading toxic plants. Here, species composition phylogenetic structure AM fungal communities colonizing roots a plant, Ligularia virgaurea, its neighborhood plants, were analyzed in patches with different individual densities L. virgaurea (represents degree). Community compositions fungi both root systems changed...

AbstractThe use of genetically modified retroviruses as agents for gene transfer has provided developmental biology with some its most elegant and compelling research stories the last few years. The growing recognition utility retrovirally derived constructs is perhaps unsurprising given that over millennia, evolution honed shuttling genetic material into native stock-in-trade this unique class animal pathogen. One application in which retroviral approaches have particularly shone analysis...

The bacterial diversity in Populus euphratica stem storage liquid samples grown Shaya County and Yuli of the Tarim River Basin was investigated. A culture-dependent (dilution spread plate method) culture-independent method (PCR-RFLP technique) were used to identify endophytic bacteria community structure composition P. Basin. Sixty-six strains isolated from on three agar media. 16S rDNA gene amplified sequenced using universal primers. Phylogenetic analysis showed that 66 belonged phyla...

Abstract In vitro embryogenic callus is a critical factor for genetic transformation of rice, especially indica varieties. this study, we investigated the relationship between polyamines, including putrescine (Put), spermidine (Spd) and spermine (Spm), browning, studied effect exogenous Put on regeneration content endogenous polyamines. addition, expression levels arginine decarboxylase gene ( Adc1 ) S-adenosylmethionine Samdc in were by quantitative Real-time PCR analysis. The results...