A5003619657- Pancreatic function and diabetes
- Diabetes and associated disorders
- Diabetes Management and Research
- Extracellular vesicles in disease
- Salmonella and Campylobacter epidemiology
- Enterobacteriaceae and Cronobacter Research
- Immune Cell Function and Interaction
- Antibiotic Resistance in Bacteria
- Galectins and Cancer Biology
University of Florida
2022-2024
Florida College
2022
Extracellular vesicles (EVs) can affect immune responses through antigen presentation and costimulation or coinhibition. We generated designer EVs to modulate T cells in the context of type 1 diabetes, a cell–mediated autoimmune disease, by engineering lymphoblast cell line, K562, express HLA-A*02 (HLA-A2) alongside costimulatory CD80 and/or coinhibitory programmed death ligand (PD-L1). presenting HLA-A2 activated CD8 + dose, antigen, HLA-specific manner. Adding PD-L1 these produced an...
Abstract During type 1 diabetes (T1D) progression, beta cells become dysfunctional and exhibit reduced first-phase insulin release. While this period of cell dysfunction is well established, its cause underlying mechanism remain unknown. To address knowledge gap, live human pancreas tissue slices were prepared from autoantibody- negative organ donors without (ND), positive for one or more islet autoantibodies (AAb+), with T1D within 0-4 years diagnosis (T1D+). Dynamic imaging physiological...
Introduction: Type 1 diabetes (T1D) is characterized by a period of β cell dysfunction prior to death. During this time, cells lack first-phase insulin response and exhibit an intolerance glucose. However, the underlying mechanisms remain unclear. Here, we use live human pancreas tissue slices (LPTS) investigate possible in T1D. Methods: LPTS from organ donors without (ND, n=13), T1D positive for one (sAAb+, n=6) or multiple T1D-relavent autoantibodies (mAAb+, n=2), individuals with...