A5035008480- scientometrics and bibliometrics research
- Academic Publishing and Open Access
- Genetics, Bioinformatics, and Biomedical Research
- Retinal Development and Disorders
- Developmental Biology and Gene Regulation
- Axon Guidance and Neuronal Signaling
- Congenital heart defects research
- Ocular Disorders and Treatments
- Zebrafish Biomedical Research Applications
- Neurogenesis and neuroplasticity mechanisms
- Computational Drug Discovery Methods
- Fibroblast Growth Factor Research
- Cancer-related gene regulation
- RNA modifications and cancer
- Academic Writing and Publishing
- Open Education and E-Learning
- Agricultural and Food Production Studies
- Health and Medical Research Impacts
- Academic integrity and plagiarism
- Religion, Society, and Development
- Interdisciplinary Research and Collaboration
- Retinal and Macular Surgery
- Immune Cell Function and Interaction
- Hedgehog Signaling Pathway Studies
- Research Data Management Practices
American Society for Microbiology
2016-2023
University of Chicago
2017-2018
University of North Carolina Hospitals
2017-2018
Yale University
2017-2018
University of Calgary
2017-2018
St. Jude Children's Research Hospital
2017-2018
University of Minnesota System
2017-2018
University of California, Irvine
2016-2017
Massachusetts Institute of Technology
2017
University of Wisconsin–Madison
2017
During pituitary organogenesis, the progressive differentiation of distinct pituitary-specific cell lineages from a common primordium involves series developmental decisions and inductive interactions. Targeted gene disruption in mice showed that Lhx3, LIM homeobox expressed throughout development, is essential for proliferation lineages. In homozygous Lhx3 mutation, Rathke's pouch formed but failed to grow differentiate; such lacked both anterior intermediate lobes pituitary. The...
As we enter the second quarter of COVID-19 pandemic, with testing for severe acute respiratory syndrome coronavirus 2 (SARS–CoV-2) increasingly available (though still limited and/or slow in some areas), are faced new questions and challenges regarding this novel virus. When to test? Whom What How often And, what do test results? Since SARS–CoV-2 is a virus, there little evidence fall back on utilization diagnostic stewardship (1). Several points need be considered begin answering these...
Science agencies and research institutions are building the infrastructure to evaluate results of federal funding scientific research.
During vertebrate eye development, the transcription factor MITF plays central roles in neuroepithelial domain specification and differentiation of retinal pigment epithelium. is not a single protein but represents family isoforms generated from common gene by alternative promoter/exon use. To address question role regulation these isoforms, we first determined their expression patterns developing mouse eyes analyzed some them genetic models. We found that two A- J-Mitf, are present...
A Scientific Integrity Consortium developed a set of recommended principles and best practices that can be used broadly across scientific disciplines as mechanism for consensus on integrity standards to better equip scientists operate in rapidly changing research environment. The two represent the umbrella under which processes should are follows: (1) Foster culture process. (2) Evidence-based policy interests may have legitimate roles play influencing aspects process, but those not...
Murine Lhx3 cDNA isolated from the mouse pituitary library encodes a LIM-type homeodomain protein that contains two tandemly repeated LIM domains and homeodomain. The identities of predicted amino acid sequences between Xenopus Xlim-3 genes are 80, 95, 97% in 1 2, homeodomain, respectively, 84% entire protein. 5'-RACE procedures genomic cloning revealed distinct N-terminal arise different exons 1a 1b. Exon sequence similar to Xlim-3, whereas exon 1b N-terminus. It is likely there...
Stefano Bertuzzi, Robert Hindges, Stina H. Mui, Dennis D.M. O'Leary, and Greg Lemke Molecular Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037 USA; Department of Neurosciences, University at San Diego, 92093 USA
The vertebrate retina and optic nerve are strikingly different in terms of their size, organization, cellular diversity, yet these two structures develop from the same embryonic neuroepithelium. Precursor cells most ventral domain this epithelium give rise only to astrocytes nerve, whereas immediately adjacent, more dorsal precursors myriad cell types retina. We provide genetic evidence that closely related, ventrally expressed homeodomain proteins—Vax1 Vax2—control neuroepithelial...
The vertebrate retina is highly ordered along both its dorsoventral (DV) and nasotemporal (NT) axes, this order topographically maintained in axonal connections to the superior colliculus of midbrain. Although graded axon guidance cues that mediate topographic mapping retinocollicular are increasingly well understood, transcriptional regulators set DV NT gradients these not. We now provide genetic evidence Vax2, a homeodomain protein expressed ventral retina, one such regulator. demonstrate...
Abstract Deletion of fibroblast growth factor receptor 3 ( Fgfr3 ) leads to hearing impairment in mice due defects the development organ Corti, sensory epithelium Cochlea. To examine role FGFR3 auditory development, cochleae from −/− were examined using anatomical and physiological methods. absence inner pillar cells an increase other cell types, suggesting that regulates fate. Defects outer hair differentiation also observed probably represent primary basis for loss. Furthermore,...
Signal transducers and activators of transcription (STATs) are critical components cytokine signaling pathways. STAT5A STAT5B (STAT5), the most promiscuous members this family, highly expressed in specific populations hypothalamic neurons regions known to mediate actions cytokines regulation energy balance. To test hypothesis that STAT5 is essential homeostasis, we used Cre-mediated recombination delete Stat5 locus CNS. Mutant males females developed severe obesity with hyperphagia, impaired...
A LIM homeobox gene, Lim5, is known to be expressed in the forebrain of Xenopus and zebrafish (Toyama et al. [1995] Dev. Biol. 170:583-593). Results from developmental comparative studies its mouse ortholog, Lhx5, indicate that this gene may play important roles development. Lhx5 expression detected most anterior portion neural tube at headfold stage, overlapping partially with Otx2 domain. After closure, as a transverse stripe, covering diencephalic primordium. This recedes restricted areas...
The subcortical telencephalon is the major source of GABAergic interneurons that, during development, tangentially migrate to cerebral cortex, where they modulate glutamatergic excitatory action pyramidal cells. transcription factor Vax1, an intracellular mediator both Shh and Fgf signaling, expressed at high levels in medial lateral ganglionic eminences (MGE LGE, respectively), septal area (SA), anterior entopeduncular (AEP) preoptic (POA). We show that Vax1 expression neuroepithelium...
The pituitary gland is an endocrine organ that developmentally derived from a fold in the oral ectoderm and juxtaposed neural ectoderm. Here, we show absence of Vax1, homeodomain transcription factor known for its role eye optic chiasm development, causes rostral to form ectopic eventually develops into separate second with all cell types neuronal fibers characteristic normal pituitary. induction associated localized expression Fgf10, gene encoding growth recruit ectodermal cells...
The subventricular zone (SVZ) is one of the sources adult neural stem cells (ANSCs) in mouse brain. Precursor proliferate SVZ and migrate through rostral migratory stream (RMS) to olfactory bulb (OB), where they differentiate into granule periglomerular cells. Few transcription factors are known be responsible for regulating NSC proliferation, migration, differentiation processes; even fewer have been found organization RMS. For this reason, we studied ventral anterior homeobox ( Vax1 ) gene...
Progress in understanding the molecular basis of melanoma has made possible identification targets with important implications clinical practice. In fact, new therapeutic approaches are emerging from basic science and it will be to implement their rapid translation into practice by active investigation. The first meeting Melanoma Research: a bridge Naples-USA, organized Paolo A. Ascierto (INT, Naples, Italy) Francesco Marincola (NIH, Bethesda, USA) took place on 6-7 December 2010. This...
During vertebrate eye development, the transcription factor MITF acts to promote development of retinal pigment epithelium (RPE). In embryos with Mitf mutations, future RPE hyperproliferates and is respecified as tissue but only in a small portion dorsal RPE. Using series genetic crosses, we show that this spatial restriction respecification brought about by persistent expression anti-retinogenic ventral homeodomain gene Vax2 dorso-proximal both Vax1 We further Vax2/Mitf double mutants...