Transforming growth factor-β1 (TGF-β1) is the most abundant TGF-β isoform detected in bone and an important functional modulator of osteoclasts. TGF-β1 can induce osteoclast apoptosis; however, apoptotic pathways involved this process are not known. We show here that human osteoclasts express both type-I type-II receptors. In absence survival factors, (1 ng/ml) induced apoptosis. The expression activated caspase-9, but caspase-8, was increased by stimulation, rate TGF-β1-induced apoptosis...

Mutations of the gene encoding p62/SQSTM1 have been described in Paget's disease bone (PDB), identifying p62 as an important player osteoclast signaling. We investigated phenotype osteoclasts differentiated from peripheral blood monocytes obtained healthy donors or PDB patients, all genotyped for presence a mutation ubiquitin-associated domain. The cohort included patients carrying not P392L and this mutation. Osteoclasts were more numerous, contained nuclei, resistant to apoptosis, had...

In this study we examined health communication anxiety (HCA) associated with language-discordant situations - that is, where people have to use their second language (L2) communicate providers who are using first (L1). We adapted existing HCA scales in order (1) assess L2 such separately for physical and mental/emotional contexts (2) control potential confounds, as not related health, allowing us obtain L2-specific measures of HCA. the relationship between willingness services situations....

Abstract Osteoprotegerin (OPG) is a secreted decoy receptor that recognizes RANKL, and blocks the interaction between RANK leading to inhibition of osteoclast differentiation activation. As OPG major inhibitor bone resorption, we wondered whether could modulate survival/apoptosis. Osteoclast apoptosis was evaluated by adding various doses human cultures obtained from cord blood monocytes. Surprisingly, decreased after OPG. We hypothesized may block its second ligand, TRAIL, which involved in...

The aim of this study was to establish a link between the highly expressed angiotensin II (Ang II) type 2 receptor (AT2) in human fetal adrenal cells and proposed apoptotic activity center gland. There an important increase DNA fragmentation with age glands fetuses from 15–20 weeks gestation. Adrenal showing characteristic internucleosomal were localized central portion zone. In cultured for 24 h, Ang II, via AT2 receptor, induced cleavage repair enzyme, poly-(ADP-ribose) polymerase....

The specific development of the human fetal adrenal gland requires cell proliferation, migration, apoptosis, and zone-specific steroidogenic activity. present work was designed to determine physiological significance previously identified spatial distribution extracellular matrix components in gland. Primary cultures cells grown on collagen IV, laminin, or fibronectin revealed that morphology affected by environmental cues. Matrices also modulated profile steroid secretion cells. Collagen IV...

The development of the human fetal adrenal gland is characterized by a gradient mitotic activity, cell migration, and apoptosis, all which dictate its particular function. Such plasticity may possibly be under control extracellular environment. goal this study was to identify components matrix in second-trimester glands. Whereas collagen IV expressed evenly throughout gland, both fibronectin laminin demonstrated mirror-imaged distribution, with higher expression central portion at periphery...

Objective. To test the hypothesis that increased concentrations of prostaglandin D 2 (PGD ) correlate with bone remodeling. Studies using isolated cells indicate PGD may be implicated in regulation homeostasis, a positive influence on anabolism. We studied patients traumatic fractures and age- sex-matched healthy controls as an vivo model Methods. Thirty-five fracture matched were recruited. Urine sera samples collected. Urinary 11ß-PGF 2α , metabolite, PGE metabolites (PGEM), serum...

Whereas collagen IV is expressed throughout the fetal adrenal gland during second trimester of human development, fibronectin, and laminin demonstrate a rather mirror-image distribution, with higher expression fibronectin in central portion at periphery gland. In present study, extracellular matrices were able to modulate profile steroid secretion primary cultures: favored cortisol following adrenocorticotropin (ACTH) or angiotensin II (Ang II) stimulation while specific AT2 receptor Ang...

The specific development of the human fetal adrenal gland requires cell proliferation, migration, apoptosis, and zone-specific steroidogenic activity. present work was designed to determine physiological significance previously identified spatial distribution extracellular matrix components in gland. Primary cultures cells grown on collagen IV, laminin, or fibronectin revealed that morphology affected by environmental cues. Matrices also modulated profile steroid secretion cells. Collagen IV...

We investigated the effect of repeated exposures to hypohydration upon cognitive performance. In a randomized crossover design, ten physically active adults completed two 4-week training blocks, one where they maintained euhydration (EUH) and other were water-restricted (DEH) during walking/running at 55% V.O2max, 40 °C. Three sessions per week performed: (1) 1 h exercise, (2) exercise until 2% or (3) 4% body mass has been lost replaced. Limited first fourth week, 12 min time-trial was...

The aim of the present study was to investigate presence and localization main G protein α-subunits in human fetal adrenal gland during second trimester gestation. Immunofluorescence studies conducted on sections from frozen glands obtained immediately after therapeutic abortion indicated that αs subunit heterotrimeric Gs detected all cell types, except for endothelial cells. otherα -subunits had a more specific pattern distribution. Indeed, theα i1–2 restricted definitive zone, whereas αi3...

ACTH is a well-known stimulus of human adrenal cells, both in the adult and fetus. Two other stimuli, acting via cAMP pathway, are also involved regulation steroidogenesis growth gland, Pituitary Adenylate Cyclase Activating Peptide (PACAP) Vasoactive Intestinal Polypeptide (VIP). The aim this study was to compare effects three peptides on production investigate their possible effect cytoskeletal organization different cell types present fetal i.e steroidogenic cells chromaffin cells. Using...

This study aimed to examine whether repeated exposures low (2%) and moderate (4%) exercise-induced hypohydration may reverse the potentially deleterious effect of on endurance performance. Using a randomized crossover protocol, ten volunteers (23 years, V˙O2max: 54 mL∙kg-1∙min-1) completed two 4-week training blocks interspersed by 5-week washout period. During one block, participants replaced all fluid losses (EUH) while in other they were restricted (DEH). Participants three exercise...