A5011198674- Antibiotic Resistance in Bacteria
- Antimicrobial Peptides and Activities
- Bacterial biofilms and quorum sensing
- Antimicrobial Resistance in Staphylococcus
- Alkaloids: synthesis and pharmacology
- Plant-based Medicinal Research
- Catalysis and Hydrodesulfurization Studies
- Bone and Dental Protein Studies
- Immune Response and Inflammation
- Pharmacological Effects and Toxicity Studies
- Antibiotic Use and Resistance
- Synthesis and Characterization of Heterocyclic Compounds
- Vibrio bacteria research studies
- Biochemical and Structural Characterization
- Cancer Research and Treatments
- Autophagy in Disease and Therapy
- Neurological Disease Mechanisms and Treatments
- RNA modifications and cancer
- Berberine and alkaloids research
- Ferroptosis and cancer prognosis
- Advanced biosensing and bioanalysis techniques
- Quinazolinone synthesis and applications
- Sirtuins and Resveratrol in Medicine
- Heat shock proteins research
- Blood groups and transfusion
Academy of Military Medical Sciences
2024
Air Force Medical University
2015-2024
Ningxia Medical University
2022
Capital Medical University
2020
Beijing Shijitan Hospital
2020
Beijing Friendship Hospital
2020
Zhejiang Hospital
2020
Tianjin Hospital
2019
Tianjin Nankai Hospital
2019
First Affiliated Hospital of Xi'an Jiaotong University
2018
Abstract New Delhi metallo-β-lactamase-1 (NDM-1) is the most prevalent type of metallo-β-lactamase and hydrolyzes almost all clinically used β-lactam antibiotics. Here we show that antimicrobial peptide thanatin disrupts outer membrane NDM-1-producing bacteria by competitively displacing divalent cations on inducing release lipopolysaccharides. In addition, inhibits enzymatic activity NDM-1 zinc ions from active site, reverses carbapenem resistance in vitro vivo. Thus, thanatin’s dual...
Abstract Given its high resistance, enhanced virulence, and transmissibility, community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) pneumonia is highly associated with morbidity mortality. Anti-virulence therapy a promising strategy that bypasses the evolutionary pressure on bacterium to develop resistance. RNAIII-inhibiting peptide (RIP), as an accessory gene regulator ( agr )-specific inhibitor, significantly restricts virulence of S. protects infected mice from death...
Thanatin (THA) displays potent antibiotic activity, especially against extended-spectrum-β-lactamase (ESBL)-producing Escherichia coli both in vitro and vivo, with minimal hemolytic toxicity satisfactory stability plasma. However, the high cost of thanatin significantly limits its development clinical application. To reduce peptide synthesis, a formulation cyclic (C-thanatin) called linear (L-thanatin) was synthesized activity evaluated vivo Results showed that C-thanatin L-thanatin MICs did...
The emergence and rapid spread of multidrug resistance in bacteria have led to the urgent need for novel antibacterial agents. Membrane permeabilization is mechanism many molecules that are being developed against gram-negative bacteria. Thus, determine efficacy a potential molecule, it important assess change bacterial membrane permeability after treatment. This study describes protocol assays outer inner using fluorescent probes N-phenyl-1-naphthylamine propidium iodide. Compared with...
Cone arrestin (CAR) is a novel member of the superfamily expressed in retinal cone photoreceptors and pineal gland. To understand regulatory mechanisms controlling its cone‐ pineal‐specific expression, to facilitate further functional studies using gene knockout approaches, we characterized genomic organization 5′‐flanking region mouse CAR (mCAR) gene. The mCAR comprised 17 exons 16 introns, encoding five alternatively spliced transcripts. A 215‐bp proximal promoter fragment containing TATA...
Developing targeted delivery nanosystems for delivering chemotherapeutic anticancer drugs specifically to cancerous tissues with improvement in the specificity of different cancer cells can result high therapeutic efficacy and low toxicity healthy tissues. Herein, we proposed synthesis a multifunctional nanodelivery system, folic acid (FA) decorating nanographene oxide (nGO) functionalized poly(ethylene glycol) (PEG), called pGO-FA, good biocompatibility performance hydrophobic...
Abstract As the persistent resistance of Staphylococcus aureus to available antibiotics is associated with high infection incidence, mortality rate and treatment cost, novel antibacterial agents innovative therapeutic targets must be developed. 3,3′-(4-Nitrobenzylidene)-bis-(4-hydroxycoumarin) (NBH), a dicoumarin derivative, was reported exert activity. This study investigated underlying mechanisms in vivo vitro activities NBH against S. . exerted bactericidal effects tested epidermidis...
The DNA tetrahedron (Td), as one of the novel DNA-based nanoscale biomaterials, has been extensively studied because its excellent biocompatibility and increased possibilities for decorating precisely. Although use Td in laboratories is well established, knowledge surrounding factors influencing preparation storage lacking. In this research, we investigated role magnesium ions, which greatly affect structure stability DNA. We assembled 1, 2, 5, 10 20 μM buffers containing different Mg2+...
Biofilm formation enhances bacterial resistance and complicates treatment. Therefore, an innovative strategy is urgently needed for the treatment of Staphylococcus aureus biofilm infectious diseases. RNAIII-inhibiting peptide (RIP), as a quorum-sensing inhibitor, inhibits S. formation. However, RIP possesses poor antibiofilm activity when used alone or at low dose in vivo. The stability can be enhanced by designing its derivatives through amino acid substitution, terminal modification,...
Multidrug resistance poses a severe threat to public health and urgently requires new solutions. The natural product chelerythrine (CHE) is benzophenanthridine alkaloid with antimicrobial potential. In this study, CHE was effective against seven gram-positive bacterial strains, the minimum inhibitory concentrations (MICs) ranged from 2 4 μg/mL. By contrast, showed inferior antibacterial activities 11 gram-negative MICs varied 16 256 We also determined synergistic/additive effects of...
Ischemic stroke is one main type of cerebrovascular disorders with leading cause death and disability worldwide. Astrocytes are the only nerve cell storing glycogen in brain, which regulate glucose metabolism handle energy supply survive neurons. Astrocyte ferroptosis contributes to neuron injury brain disorders. N-myc downstream-regulated gene 2 (NDRG2) has been implicated progression diseases, including ischemic stroke. However, whether NDRG2 could affect astrocytes during remains largely...
New Delhi metallo-β-lactamase-1 (NDM-1)-producing bacteria are resistant to nearly all available β-lactam antibiotics and have become a public health threat. Antibiotic resistance often carries fitness costs, which typically manifest as reduced bacterial growth rate. Here, we investigated the mechanism of cost in NDM-1-producing bacteria. Our findings revealed that strains expressing bla
Piper nigrum L. and P. longum are widely used in various medicinal formulations. The dichloromethane fraction of (DF) can prevent cerebral ischemic injury although the underlying mechanisms obscure. aim this study was to evaluate potential neuroprotective effects DF on a rat model permanent middle artery occlusion (pMCAO) assess molecular mechanisms. Animals were administered with (50, 100, 150 mg/kg) or nimodipine (12 6 h after pMCAO for 14 consecutive days via intragastric gavage. In vitro...
Increasing evidence has demonstrated the essential role of lncRNAs in tumorigenesis. LINC00514, a novel lncRNA, was reported to be promoter malignant behaviors cancer, but pituitary adenoma (PA), its biological functions remain unclear. Herein, we measured LINC00514 expression by RT-qPCR analysis which indicated significant elevation human PA tissues. Moreover, effect silencing on cell proliferation and invasion was, respectively, examined CCK-8 transwell assays. The results showed that...