A radioimmunoassay for rat growth hormone capable of measuring levels in plasma is described detail. Several experimental conditions were studied, both to validate the method and determine factors which regulate GH secretion this species. In fasting Sprague-Dawley rats weighing 150-200 g, mean concentration females was 94.2±17.3, males, 54.9±12.0 mμg/ml. lactating determined be normal range females, thereby providing evidence that assay does not detect prolactin. elevated pregnant rats....

Abstract Synthetic thyrotropin-releasing hormone (TRH) was administered by single intravenous injection to 79 healthy male volunteers and seven patients with secondary hypothyroidism. Plasma thyrotropin (TSH), growth (GH), luteinizing (LH), follicle-stimulating (FSH) cortisol concentrations were measured before after TRH administration. In the normal subjects, mean base-line TSH concentration 4.5 μU per milliliter. rose promptly a peak of 14.0 milliliter 30 minutes injection. No rises...

Previous studies have indicated that one or more GH-dependen t somatomedins (SMs) are either synthesized at least released by the liver. Employing isolated rat liver perfusion technique and a competitive protein-binding assay to measure of SMs [insulin-like growth factor (IGF)] its carrier protein (CP), net cumulative release these peptides from normal hypophysectomized (hypox) livers has been studied. The IGF concentration in perfusates seven increased rapidly during initial 2 h gradually...

Eight patients with serious depression were given 1000 µg. of thyrotropin-releasing hormone (TRH) or saline intravenously for three days in a double-blindstudy. All then received TRH daily the next seven days. The group receiving showed greatest improvement; only one patient improved substantially while TRH. Plasma thyrotropin and prolactin responses to distinctly diminished most severely depressed patients. authors suggest that primary value may be as diagnostic tool differentiating among...

The recent isolation of highly purified human pituitary luteinizing hormone (LH) has permitted the development a sensitive and specific radioimmunoassay for this in plasma. Results immunoassay system employing anti-LH serum agree closely with previous reports measurement plasma LH which immunoassays cross-reactive antisera to chorionic gonadotropin were used. bioassay several crude partially urinary extracts show acceptable agreement. sensitivity (0.2 mμg/ml) is adequate measure levels...

Thyrotropin-releasing hormone (TRH) was administered iv to 8 patients with renal failure, normal men, and 5 children. Injection of 1600 μg TRH as a bolus healthy adults or smaller dose children resulted in pattern TSH release. The prolonged release which occurred after stat injection 400 the greatly diminished function similar that found 4-hr infusion men. In men receiving infusion, levels began diminish 2 hr, despite continued for an additional hr. An unexpected increase hGH also 7 failure TRH.

To study the possible mechanisms involved in growth retardation associated with hypothyroidism, serum T4, GH, GH-dependent somatomedin, insulin-like factor (IGF), and its carrier protein (CP) were measured hypothyroid rats their age-matched controls. Three groups of studied: infant, immature, adult. Marked hypothyroidism (serum T4(<1 μg/dl) was produced experimental animals by providing them drinking water containing 0.05% propylthiouracil. Infant immature weighed markedly less than normal...

Five of eight patients with documented ataxia telangiectasia had an unusual form diabetes mellitus, characterized by marked hyperglycemia, resistance to ketosis, absence glycosuria and markedly elevated plasma insulin levels in response the administration glucose or tolbutamide. Moreover, blood both exogenous endogenous was decreased, signifying similar that found pregnancy, acromegaly obesity. In addition, five subjects evidence hepatic disease, which has not previously been reported...

We have used [32P]-labeled human and mouse IGF-I cDNA probes to identify three hepatic transcripts (8.0, 1.8 1.1 kilobase) in rats quantify nutritionally-induced changes. During fasting, the major (8.0 transcript descreased progressively after 6 h, at 30 h was only 39% as abundant nonfasted control. Refeeding animals fasted 24 caused a marked rise 8.0-kb mRNA by an 18-fold increase rw The two minor also appeared behave similar manner. Serum concentrations paralleled levels of during periods...

Synthetic 3H-labeled luteinizing hormone-releasing hormone (LH-RH) with a specific activity of 18.3 Ci/mM and the same biological as pure porcine LH-RH was used to study half-life, metabolism urinary excretion in man. Seven healthy men were given rapid iv injection 42 μCi 3H-LH-RH, followed 6 subjects by 250 μg unlabeled LH-RH. Frequent samples blood urine collected. There mean maximum increase plasma levels LH 963% FSH 312% obtained 30 min after injection. A plot disappearance times...

The availability of synthetic TRH has permitted studies TSH release in various disorders the hypothalamic-pituitary axis. Forty-five patients with pituitary, hypothalamus or basal ganglia were studied. response to 400 μg intravenous was normal all but two 19 acromegalics, high 6 chromophobe adenomas, low undetectable 7 panhypopituitarism, 3 galactorrhea and amenorrhea 5 parkinsonism, markedly elevated hypothalamic hypothyroidism. Unexpectedly, plasma GH also rose significantly after...

Data from studies in diabetic rodents and evidence clinical situations of severe resistance to insulin suggest that insulin-like growth factor I (IGF-I) is able at least partly overcome resistance. To assess the efficacy recombinant human IGF-I subjects with most common form resistance, e.g., obese, type II patients, we administered (rhIGF) doses 120 160 μg/kg twice daily for 4–52 days seven such individuals who had been treated previously high (&amp;gt; 0.7 U·kg−1 · day−1). Four patients...

OBJECTIVE Randomized, double‐blinded, placebo‐controlled, clinical trial to determine the effectiveness of palatal implants for treatment mild/moderate obstructive sleep apnea/hypopnea syndrome (OSAHS). STUDY DESIGN AND SETTING Sixty‐two non‐obese adults with history snoring, daytime sleepiness, and OSAHS, were randomized receive (n = 31) or placebo procedure 31). Complete follow‐up including quality life (QOL, SF‐36), snoring visual analog scale (VAS), Epworth Sleepiness Scale (ESS) data...

The results of nerve repair with fibrin glue and microsuture were evaluated in rat transection models. Ninety Wistar-Furth median nerves exposed, transected, repaired an end-to-end fashion one four substances/techniques: 1) human sealant (Quixil); 2) autologous graft 3) bovine (Tissucol); 4) nylon microsuture, epineurial technique. Histologic analyses performed at 3-, 6-, 9-month postoperative intervals, factors included: presence inflammatory cells (i.e., macrophages T cells); number...

Background The purpose of this study was to determine how functional endoscopic dilatation the sinuses (FEDS) compares with sinus surgery (FESS) in a select group patients respect (1) elimination symptoms, (2) patient satisfaction, (3) postoperative narcotic use, and (4) cost. A retrospective performed 70 chronic rhinosinusitis who underwent FEDS or FESS as primary revision treatment. Methods Symptoms satisfaction based on Sino-Nasal Outcome Test (SNOT-20) questionnaires global assessment,...

The hypothesis is tested that luteinizing hormone (LH) and follicle stimulating (FSH) may be released from the anterior pituitary in response to a psychological state of sexual arousal. LH levels 10 male volunteers were found higher after viewing sexually arousing film than control film. magnitude was positively correlated with subjective evaluation FSH tended same direction bu predominant unexpected finding for this consistently lower during first session, when anxiety high, second less,...

Twenty-two patients were studied during hospital admission for cardiac catheterization. Two independent observers rated their behavior at 15-min intervals over the 3-hour catheterization procedure in categories of affect, arousal and degree interpersonal engagement. These data used together with information obtained from retrospective interviews to assign each patient one behavioral groups designated anxious-engaged, anxious-not engaged, depressed calm. Plasma levels growth hormone,...

We have studied the effect of a calorie-restricted diet on growth young rats and serum levels GH, somatomedins (insulin-like factors I II), total T4 T3, free index, corticosterone. Experimental consumed same quantities protein carbohydrate as control animals, but less fat, so that their calorie intake was approximately 76% controls. The mean (±sem) GH level in experimental group (78 ± 21 ng/ml) not significantly different from (89 31 ng/ml). In contrast, insulin-like factor IGF-I, while...

Restricting male rats to one-half normal food intake from day 25 until 60 of life significantly delayed all aspects growth that were measured. Body weights reduced by 50% and pituitary hormone (GH) plasma GH levels severly compared with corresponding values ad libitum controls. Underfeeding also partially inhibited testicular accessory organ growth. If underfeeding was combined blinding, a more marked inhibition occurred. The effect combining blinding dramatic than the each treatment...