A5019968585- RNA and protein synthesis mechanisms
- Retinal and Macular Surgery
- DNA and Nucleic Acid Chemistry
- Retinal Diseases and Treatments
- Protein Structure and Dynamics
- Education, Psychology, and Social Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Law, logistics, and international trade
- Taxation and Legal Issues
- Immune Response and Inflammation
- Medical and Biological Ozone Research
- Intracranial Aneurysms: Treatment and Complications
- RNA modifications and cancer
- Pancreatitis Pathology and Treatment
- RNA Research and Splicing
- Enzyme Structure and Function
- Intraocular Surgery and Lenses
- Genomics and Chromatin Dynamics
- Clinical Nutrition and Gastroenterology
- European Cultural and National Identity
- Recommender Systems and Techniques
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Environmental Science and Water Management
- Plant-based Medicinal Research
- Liver Disease Diagnosis and Treatment
University of Chemistry and Technology, Prague
2011-2018
Université Paris Cité
2013
Brno University of Technology
2009-2010
Oklahoma State University Center for Health Sciences
1986-1987
University of California, Los Angeles
1984
University Hospital of Lausanne
1982-1984
Hôpital Orthopédique de la Suisse Romande
1983
Medical University of Vienna
1982
University Hospital of Bern
1982
Hôpital Nestlé
1982
To investigate the principles driving recognition between proteins and DNA, we analyzed more than thousand crystal structures of protein/DNA complexes. We classified protein DNA conformations by structural alphabets, blocks [de Brevern, Etchebest Hazout (2000) (Bayesian probabilistic approach for predicting backbone in terms blocks. Prots. Struct. Funct. Genet., 41:271-287)] dinucleotide conformers [Svozil, Kalina, Omelka Schneider (2008) (DNA their sequence preferences. Nucleic Acids Res.,...
In the process of recodifying Czech company law, only an insufficient attention is being paid to larger European context. This circumstance might result in serious deficiencies final proposal new Commercial Act. As a future EU Member State, Republic cannot afford ignore sometimes radical corporate law reform undertaken both national jurisdictions other States, and EC itself. After break-through decisions ECJ Centros most recently Ueberseering Cases with instruments opening gates for...
The DNA sugar–phosphate backbone has a substantial influence on the structural dynamics. Structural biology and bioinformatics studies revealed that in experimental structures samples wide range of distinct conformational substates, known as rotameric families. Their correct description is essential for methods used to model nucleic acids be Achilles heel force field computations. In this study we report benchmark database MP2 calculations extrapolated complete basis set atomic orbitals with...
DNA is a structurally plastic molecule, and its biological function enabled by adaptation to binding partners. To identify the structural polymorphisms that are possible in such adaptations, dinucleotide structures of 60 000 steps from sequentially nonredundant crystal were classified an automated protocol assigning 44 distinct (conformational) classes called NtC (for Nucleotide Conformers) was developed. further facilitate understanding structure, assembled into alphabet CANA...
The recent discoveries of regulatory non-coding RNAs changed our view RNA as a simple information transfer molecule. Understanding the architecture and function active molecules requires methods for comparing analyzing their 3D structures. While structural alignment short is achievable in reasonable amount time, large structures represent much bigger challenge. Here, we present SETTER web server structure pairwise comparison utilizing (SEcondary sTructure-based TERtiary Structure Similarity...
Abstract Background A growing number of crystal and NMR structures reveals a considerable structural polymorphism DNA architecture going well beyond the usual image double helical molecule. is highly variable with dinucleotide steps exhibiting substantial flexibility in sequence-dependent manner. An analysis conformational space backbone enhancement our understanding dependencies are therefore important for full comprehension polymorphism. Results detailed classification local conformations...
We analyzed the structural behavior of DNA complexed with regulatory proteins and nucleosome core particle (NCP). The three-dimensional structures almost 25 thousand dinucleotide steps from more than 500 sequentially non-redundant crystal were classified by using alphabet CANA (Conformational Alphabet Nucleic Acids) associations between ten letters sixteen sequences investigated. showed features discriminating specific non-specific binding to proteins. Important is role two forms, A-DNA,...
Understanding the architecture and function of RNA molecules requires methods for comparing analyzing their tertiary quaternary structures. While structural superposition short RNAs is achievable in a reasonable time, large structures represent much bigger challenge. Therefore, we have developed fast accurate algorithm pairwise structure called SETTER implemented it web server. However, though biological relationships can be inferred by alignment, key features preserved evolution identified...
Neutrophil myeloperoxidase (MPO) activity was analyzed by a semi-quantitative cytochemical method in 268 subjects divided into several groups. 17 with significantly reduced MPO were found: 11 of 23 the preleukemia group, 2/14 AMLs, 1/20 myeloproliferative syndrome, 1/7 carcinoma bone marrow metastases, 1/33 diabetes mellitus and 1/50 normals. Only comparison to normal group (p < 0.005). The high frequency acquired deficiency represents useful criterium for this diagnosis. Furthermore, these...
Presented paper is focused on description of a system design for monitoring patient head after special ophthalmological operation called Vitrectomy. For optimal recovery correct position the during time important. The designed consists telemetric unit and communication unit. provides periodical tilt sensing using digital MEMS accelerometer. angle corresponding are stored in built-in memory. also warned by sound alarm when posture incorrect. second part this which with via wireless interface....
Experience developing multidisciplinary bachelor's and master's curricula involving intertwined chemistry, informatics, librarianship–editorship skills is described. The curriculum was created in close cooperation of academic staff, library the publishing house staff (Institute Chemical Technology Prague: a sole publisher chemical literature Czech Republic), with aim to educate new generation information retrieval e-publishing professionals science–technology–medicine (STM) field. This...
The automatic head position monitoring system is designed for patient after special ophthalmological operation called Vitrectomy. In the final phase of this a small amount vitreous replaced with bubble expansive gas or oil to push on recovering parts retina. Correct during recovery time therefore important. presented device provides periodical and logging posture head. warned by sound alarm when his incorrect. digital three-axis MEMS accelerometer has been used tilt sensing. Digital signal...