A5101475945- Fungal Biology and Applications
- Polysaccharides and Plant Cell Walls
- Mycorrhizal Fungi and Plant Interactions
- Medicinal plant effects and applications
- Chromatography in Natural Products
- Phytochemicals and Antioxidant Activities
- Phytochemistry and Bioactivity Studies
- Chemical synthesis and alkaloids
- Pharmacological Effects of Natural Compounds
- Herbal Medicine Research Studies
- Molecular spectroscopy and chirality
- Biocrusts and Microbial Ecology
- Phytochemistry and Biological Activities
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Phytochemical compounds biological activities
- Medicinal Plant Pharmacodynamics Research
- Slime Mold and Myxomycetes Research
- biodegradable polymer synthesis and properties
- Microbial Natural Products and Biosynthesis
- Nanocluster Synthesis and Applications
- Drug Transport and Resistance Mechanisms
- Medicinal Plants and Bioactive Compounds
- Fermentation and Sensory Analysis
- Free Radicals and Antioxidants
Shanghai Academy of Agricultural Sciences
2015-2024
Second Affiliated Hospital of Zhengzhou University
2020-2022
Zhengzhou University
2022
Ministère de l'Agriculture et de la Souveraineté alimentaire
2019-2021
Academy of Medical Sciences
2020
Tianjin University of Science and Technology
2018
Ministry of Agriculture and Rural Affairs
2016
Abstract The use of natural substances derived from traditional Chinese medicine and plants as safe radiosensitizing adjuvants is a new trend for cancer radiotherapy. Ganoderma lucidum has been used with history more than 2000 years. Ganoderic acid T (GAT) typical triterpene G. lucidum, which strong cytotoxicity to cells, but whether it radiation sensitization effect not explored. In this work, we treated the HeLa cells different concentrations GAT before exposure gamma-ray investigated its...
A novel enhanced triterpenes fermentation production process by Ganoderma lucidum G0119 with the addition of oleic acid in medium has been developed and optimized. All six exogenous additives tested were found to exhibit stimulatory effect on mycelial growth biosynthesis G. . The results show that had significant role promoting production. optimal concentration time determined be 30 mL/L 0 h, respectively. Furthermore, three factors influencing identified as glucose, magnesium sulfate...
Ganoderma lucidum mycelia are rich in active substances such as triterpenoids and sterols. However, reports on the development of effective submerged fermentation processes lacking resulting total triterpene sterol yield is still quite low. In this study, a new G. strain G0017 mycelium isolated by screening was studied 3-L fermenter to investigate effect aeration rate liquid production By fitting specific mycelial growth triterpenoid model, an multistage control process for developed. This...
Kinetic models and temperature control strategy were established to reflect the effect of (22 °C-30 °C) on flavonoid production Phellinus baumii (P. baumii) in 6-L fermentor. A modified Logistic equation, Hinshelwood model, Luedeking Piret equation used describe mycelial growth product formation. The influence estimated kinetic parameters was further studied by regression analysis. Based analysis, new proposed. Briefly, at 0-43 H, decreasing (30 °C-28 can shorten lag phase growth, 43-90...
Ganoderic acids (GAs) are major functional components of Ganoderma lucidum . The study aimed to breed a new G. strain with increased contents individual GAs. Two mating-compatible monokaryotic strains, 260125 and 260124, were successfully isolated from the dikaryotic CGMCC 5.0026 via protoplast formation regeneration. Vitreoscilla hemoglobin gene ( vgb ) squalene synthase sqs overexpressed in 260124 strain, respectively. Mating between overexpressing resulted hybrid sqs-vgb. maximum...
Triterpenes are important anticancer agents produced by batch submerged liquid fermentation, with the medicinal mushroom Ganoderma lucidum ACCC G0119, which was investigated under various dissolved oxygen levels varying agitation speeds. Three kinetic parameters were analyzed: specific mycelial growth rate (μsmg), glucose consumption (qsgc), and triterpene production (qstp). High concentration, yield, productivity of triterpenes achieved developing a simple reproducible two-stage speed...
The mushroom today known as Ganoderma lingzhi has been used for centuries in the countries of Eastern Asia a very important medicinal mushroom. It prefers growing on rotten wood broadleaf trees and is mainly distributed tropics subtropics. Its relative G. lucidum occurs naturally almost all Earth, it colonizes mostly oak beech Central Europe. are similar species. To obtain qualitative parameters lucidum, several strains (five five ones) were chosen cultivated both Slovakia China, using chip...
Ethanolic extracts of fruit bodies 5 species (8 strains) the genus Phellinus, and isolated fractions derived from 1 these (Ph. Baumii PB-10), were evaluated for antioxidant activity, inhibitory effects on growth human tumor cells, capacity to protect PC12 cells against H2O2-induced oxidative damage. Extracts all 8 strains Phellinus spp. exhibited activity protected damage at different magnitudes potency. The strongest was by Ph. PB-10, with recorded IC50 values superoxide radical hydrogen...
Abstract A comprehensive and sensitive method combining ultra-performance liquid chromatography with tandem mass spectrometry was developed for the quantification of characteristic triterpenoids in Ganoderma mycelia. Eight ganoderic acids previously isolated from mycelia lingzhi were separated a binary mobile phase on reversed-phase C18 column. triple quadrupole spectrometer equipped an electrospray ionization source used as detector negative ion mode. Identification quantitation target...
New extraction technology of chenodeoxycholic acid from duck bile paste by calcium salt was investigated. The optimum conditions were determined orthogonal experimental design. results indicated that influencing factors on the efficiency as follows: hydrogen peroxide, methyl alcohol, glacial acetic acid, and chloride. extracting 1000 mL amount 50 500 20% chloride, 600 60% for a quantity paste. yield 30%.
To fully analyze the composition of volatile oil extracted from Tremella fuciformis, hydrodistillation (HD) and solid phase microextraction (SPME) were adopted simultaneously. In both cases, analysis was carried out using gas chromatography-mass spectrometry antioxidant activity determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) method with rutin as a positive control. Nineteen components in HD 68 SPME identified, respectively. Moreover, obtained T. fuciformis indicated that aromatic...
A known compound, 5-(hydroxymethyl) furan-2-carbaldehyde, and a novel 3-isobutyl-1-methoxy-4-(4'-(3-methylbut-2-enyloxy)phenyl)-1H-pyrrole-2,5-dione were isolated from spent broth submerged cultures of Taiwanofungus camphoratus. Their structures elucidated by nuclear magnetic resonance (1H, 13C, 2D) mass spectra. These compounds inhibited the proliferation K562 HepG2 tumor cells in vitro.