A5037164049- Developmental Biology and Gene Regulation
- Congenital limb and hand anomalies
- Zebrafish Biomedical Research Applications
- Congenital heart defects research
- Axon Guidance and Neuronal Signaling
- Hedgehog Signaling Pathway Studies
- Nerve injury and regeneration
- Connective tissue disorders research
- Wnt/β-catenin signaling in development and cancer
- Marine animal studies overview
- TGF-β signaling in diseases
- Cleft Lip and Palate Research
- Genetic Syndromes and Imprinting
- Epigenetics and DNA Methylation
- Genomic variations and chromosomal abnormalities
- Pluripotent Stem Cells Research
- Cancer-related gene regulation
- Urological Disorders and Treatments
- Advanced Fluorescence Microscopy Techniques
Appalachian State University
2020
University of Washington
2012-2015
University of Florida
2006-2010
Florida College
2009
Cancer Genetics (United States)
2009
Malformations of the external genitalia are among most common congenital anomalies in humans. The urogenital and anorectal sinuses develop from embryonic cloaca, penis clitoris genital tubercle. Within tubercle, endodermally derived urethral epithelium functions as an organizer expresses sonic hedgehog (Shh). Shh knockout mice lack have a persistent cloaca. This identified early requirement for Shh, but precluded analysis its later role We conducted temporally controlled deletions report...
The vertebrate body forms from a multipotent stem cell-like progenitor population that progressively contributes newly differentiated cells to the most posterior end of embryo. How balances proliferation and other cellular functions is unknown due difficulty analyzing cell division in vivo. Here, we show compartmentalized at embryo during early zebrafish development by regulated expression cdc25a, key controller mitotic entry. Through use transgenic line misexpresses this...
Anterior to posterior growth of the vertebrate body is fueled by a posteriorly located population bipotential neuro-mesodermal progenitor cells. These cells have limited rate proliferation, and their maintenance critical for completion anterior-posterior axis. How these leave state commit differentiation largely unknown, in part because widespread modulation factors essential this process causes organism-wide effects. Using novel assay, we show that Tbx16 (Spadetail) capable advancing...
Expression of Sonic Hedgehog ( Shh ) in the posterior mesenchyme developing limb bud regulates patterning and growth by activation (Hh) signaling pathway. Through analysis Hh target genes, it has been shown that mesoderm is required for normal development to occur. In contrast, stated ectoderm cannot occur because components pathway genes have not found this tissue. However, recent array-based data identified both necessary activate targets ectoderm. Using immunohistochemistry various...
The neurotrophic tyrosine kinase receptor (Ntrk) gene family plays a critical role in the survival of somatosensory neurons. Most vertebrates have three Ntrk genes each which encode Trk receptor: TrkA, TrkB, or TrkC. function receptors is modulated by p75 neurotrophin (NTRs). Five ntrk and one NTR (ngfrb) been discovered zebrafish. To date, expression these initial stages neuron specification not investigated. present work used whole mount situ hybridization to analyze five ngfrb zebrafish...