A5090561198- Probiotics and Fermented Foods
- Gut microbiota and health
- Protein Hydrolysis and Bioactive Peptides
- Microbial Metabolites in Food Biotechnology
- Meat and Animal Product Quality
- Clostridium difficile and Clostridium perfringens research
- Enzyme Production and Characterization
- Heavy Metal Exposure and Toxicity
- Milk Quality and Mastitis in Dairy Cows
- GABA and Rice Research
- Pediatric health and respiratory diseases
- Infant Nutrition and Health
- Listeria monocytogenes in Food Safety
- Antimicrobial Resistance in Staphylococcus
- Biochemical and Structural Characterization
- Identification and Quantification in Food
- Child Nutrition and Feeding Issues
- Gastrointestinal motility and disorders
- Extracellular vesicles in disease
- Biochemical effects in animals
- Biochemical Analysis and Sensing Techniques
- Advanced Glycation End Products research
- Salmonella and Campylobacter epidemiology
- Bacteriophages and microbial interactions
- SARS-CoV-2 detection and testing
University of Belgrade
2016-2025
Institute of Molecular Genetics and Genetic Engineering
2008-2013
Institute of Molecular Genetics
2010-2011
While gut microbiota dysbiosis has been linked with autism, its role in the etiology of other neurodevelopmental disorders (NDD) is largely underexplored. To our knowledge this first study to evaluate diversity and composition 36 children from Republic Serbia diagnosed NDD 28 healthy children. The results revealed an increased incidence potentially harmful bacteria, closely related Clostridium species, patient group compared Control group: Desulfotomaculum guttoideum (P < 0.01),...
Probiotic bacteria are recognized for their health-promoting properties, including maintenance of gut epithelial integrity and host immune system homeostasis. Taking into account the beneficial effects GABA, presence gadB gene, encoding glutamate decarboxylase that converts L-glutamate to was analyzed in Lactic Acid Bacteria (LAB) natural isolates from Zlatar cheese. The results revealed 52% tested Lactobacillus spp. 8% Lactococcus harbor gene. Qualitative quantitative analysis GABA...
The aim of this study was to determine the role an exopolysaccharide produced by natural dairy isolate Lactobacillus paracasei subsp. BGSJ2-8, in adhesion intestinal epithelial cells and a decrease Escherichia coli's association with Caco-2 cells. Annotation BGSJ2-8 genome showed presence gene cluster, epsSJ, which encodes biosynthesis strain-specific EPS-SJ, detected as two fractions (P1 P2) size exclusion chromatography (SEC) coupled multi-angle laser light scattering (MALLS) detection....
Enterococci have controversial status due to their emerging role in nosocomial infections and transmission of antibiotic resistance genes, while some enterococci strains are used as probiotics for humans animals starter cultures dairy industry. In order improve our understanding factors involved the safe use potential probiotics, susceptibility, virulence probiotic traits 75 isolates belonging Enterococcus durans (50), En. faecium (15), faecalis (6), italicus (3) hirae (1) were evaluated....
Abstract In recent years the role of extracellular vesicles (EVs) Gram-positive bacteria in host-microbe cross-talk has become increasingly appreciated, although knowledge their biogenesis, release and host-uptake is still limited. The aim this study was to characterize EVs released by dairy isolate Lactiplantibacillus plantarum BGAN8 gain an insight into putative mechanism uptake intestinal epithelial cells. cryo-TEM observation undoubtedly demonstrated (20 140 nm) from surface BGAN8, with...
Abstract The characterization of mechanisms involved in the positive effects probiotic bacteria various pathophysiological conditions is a prerogative for their safe and efficient application biomedicine. We have investigated immunological live bacteria-free supernatant collected from GABA-producing Lactobacillus brevis BGZLS10-17 on Concanavalin A-stimulated mesenteric lymph node cells (MLNC), an vitro model activated immune cells. shown that GABA containing GABA-free strong...
Eleven Lactobacillus strains with strong aggregation abilities were selected from a laboratory collection. In two of the strains, genes associated capability plasmid located and found to strongly correlate collagen binding. The gene encoding auto-aggregation-promoting protein (AggLb) paracasei subsp. BGNJ1-64 was cloned using novel, wide-range-host shuttle cloning vector, pAZILSJ. clone pALb35, containing 11377-bp DNA fragment, SacI library for its ability provide carriers phenotype....
ABSTRACT Lactobacillus paraplantarum BGCG11, a putative probiotic strain isolated from soft, white, artisanal cheese, produces high-molecular-weight heteropolysaccharide, exopolysaccharide (EPS)-CG11, responsible for the ropy phenotype and immunomodulatory activity of strain. In this study, 26.4-kb region originating pCG1 plasmid, previously shown to be production EPS-CG11 phenotype, was cloned, sequenced, functionally characterized. 16 open reading frames (ORFs), encoding enzymes EPS-CG11,...
ObjectiveAltering dysbiotic gut flora through synbiotic supplementation has recently been recognized as a potential treatment strategy to reduce the levels of gut-derived uremic toxins and decrease inflammation. Assessing its efficacy safety main goal our randomized, double-blind, placebo-controlled study.MethodsA total 34 nondialyzed chronic kidney disease patients, aged ≥18 years, with an estimated glomerular filtration rate between 15 45 mL/minute, were randomized either intervention...
The gut microbiota is deeply interconnected with the brain, a phenomenon often referred to as gut-brain axis. Dysfunction in microbiota-gut-brain axis can cause various neurological and psychiatric disorders associated chronic inflammation dysbiosis. Therefore, cultivation of anaerobic human strains, characterization their safety status immunomodulatory potential could contribute deciphering molecular mechanisms underlying communication revealed biotherapeutic potential. However, poor...
Enterococci represent the most controversial group of dairy bacteria. They are found to be main constituent many traditional Mediterranean products and contribute their characteristic taste flavor. On other hand, during last 50 years antibiotic-resistant enterococci have emerged as leading causes nosocomial infections worldwide. The aim this study was determine diversity, technological properties, antibiotic susceptibility virulence traits 636 previously isolated from 55 artisan 12 locations...
In this study, for the first time, comprehensive analysis of antiproliferative and antioxidant activities ramson, followed by its associated microbiota health-promoting effects lactic acid bacteria (LAB), was performed. Ramson ( Allium ursinum ) is recognized as a medicinal plant with long history use in traditional medicine due to antimicrobial activity. study influence vitro gastrointestinal digestion on cytotoxic activity A. extracts against human malignant cell lines demonstrated. Seven...
The aim of this study was to investigate the in vitro probiotic potential dairy yeast isolates from artisanal cheeses manufactured Serbia and Croatia.Twelve strains isolated fresh soft white brined Croatia were used study. Survival chemically-simulated gastrointestinal conditions, adherence epithelial intestinal cells proliferation gut-associated lymphoid tissue (GALT) evaluated.The results revealed that two Kluyvereomyces lactis ZIM 2408 2453 grew above one log unit (Δ CFU/ml) complex...
The understanding of mechanisms interactions between various bacterial cell surface proteins and host receptors has become imperative for the study health promoting features probiotic enterococci. This study, first time, describes a novel enterococcal aggregation protein, AggE, from Enterococcus faecium BGGO9-28, selected laboratory collection isolates with auto-aggregation phenotypes. Among them, En. BGGO9-28 showed strongest auto-aggregation, adhesion to components ECM biofilm formation....
Cadmium (Cd) ranks seventh on the list of most significant potential threats to human health based its suspected toxicity and possibility exposure it. It has been reported that some bacterial exopolysaccharides (EPSs) have ability bind heavy metal ions. We therefore investigated capacity eight EPS-producing lactobacilli adsorb Cd in present study, Lactiplantibacillus plantarum BGAN8 was chosen as best candidate. In addition, we demonstrate an EPS derived from (EPS-AN8) exhibits a high...
Cadmium (Cd) is a highly toxic metal that distributed worldwide. Exposure to it correlated with vast number of diseases and organism malfunctions. Exopolysaccharides (EPS) derived from Lactiplantibacillus plantarum BGAN8, EPS-AN8, previously showed great potential for the in vitro protection intestinal cells this metal. Here, we investigated food supplemented EPS-AN8 protect rats hazardous effects Cd exposure. After thirty days exposure lower (5 ppm) higher (50 ppm)-Cd doses, administration...
The strain Lactobacillus paracasei subsp. BGUB9 that was isolated from traditionally homemade hard cheese produces bacteriocin designated as BacUB9, with an approximate molecular mass of 4 kDa. Biochemical characterization and the antimicrobial activity test BacUB9 were performed. onset biosynthesis observed at end exponential phase growth. Bacteriocin UB9 retained within pH range 1 to 10 after treatment 100oC for 30 min. is susceptible proteolytic enzymes. has a very narrow spectrum,...