A5057811819- Aquaculture Nutrition and Growth
- Aquaculture disease management and microbiota
- Enzyme Production and Characterization
- Viral Infectious Diseases and Gene Expression in Insects
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Protein purification and stability
- Enzyme Catalysis and Immobilization
- Insect and Arachnid Ecology and Behavior
- Microencapsulation and Drying Processes
- Bacterial Genetics and Biotechnology
- Meat and Animal Product Quality
- Heat shock proteins research
- Methane Hydrates and Related Phenomena
- Machine Learning in Bioinformatics
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Peptidase Inhibition and Analysis
- Insect symbiosis and bacterial influences
- Protein Hydrolysis and Bioactive Peptides
- Probiotics and Fermented Foods
Hanoi University of Science and Technology
2019-2024
Martin Luther University Halle-Wittenberg
2005
Newcastle University
2002
Abstract Fed‐batch production of recombinant β‐galactosidase in E. coli was studied with respect to the specific growth rate at induction. The cultivations were designed induce protein by IPTG a glucose feed corresponding high (μ = 0.5 h −1 ) or low 0.1 rate. Protein approximately 100% higher rate, resulting accumulation up 30% total cell protein. Transcription analysis showed that β‐galactosidase‐specific messenger RNA immediately formed after induction (<5 min), but amount same both...
BACKGROUND: The permanently impaired protein folding during recombinant production resembles the stress encountered at extreme temperatures, under which condition putative holding chaperones, IbpA/IbpB, play an important role. We evaluated impact of ibpAB deletion or overexpression on responses and inclusion body metabolism yeast alpha-glucosidase in Escherichia coli. RESULTS: Deletion ibpAB, is innocuous physiological conditions, culture growth production. At higher accumulation proteins...
During fast production in recombinant Escherichia coli, the enzyme alpha-glucosidase from Saccharomyces cerevisiae accumulates partially as inclusion bodies. The bodies are reactivated inside cell upon temperature downshift. This vivo reactivation was most efficient on complex medium with body at 42 degrees C and 30 C, yielding volumetric activities 85% higher than those of extended isothermal low temperature. On defined medium, however, slow. In fed-batch cultivations, feeding controls...
Lignocellulose is a renewable and enormous biomass resource, which can be degraded efficiently by range of cocktails carbohydrate-active enzymes secreted termite gut symbiotic bacteria. There an urgent need to find with novel characteristics for improving the conversion processes in production lignocellulosic-based products. Although various studies dedicated genus Cellulosimicrobium as symbiont, genetic potential related plant biomass-acting exopolysaccharides has been fully untapped...
Chitin from crustacean waste can be the future substrate for bioindustry towards circular economy concept, especially countries having large production of sea products like Vietnam. However, chemical conversion chitin into monomers implies high amounts NaCl in a mixture with glucosamine, N-acetyl and acetate. Thus, bacterial strain that tolerate salt concentration, use as sole carbon source such Vibrio natriegens holds great potential producing bioproducts derivatives. In this study, V....
Vibrio natriegens has recently been identified as a promising host for the biotechnology industry thanks to its inherent qualities, which include fast growth rate, non-pathogenicity humans, and versatility in using substrates. These advantages have led potential use of V. biosynthesis several products. Basically, industrial scale requires fermentation or cultivation processes be conducted at high substrate biomass concentrations maximize final retrieved product. However, studies on cell...
The intracellular aminopeptidase activities and volatile compound profile of isolated halophilic lactic acid bacteria Tetragenococcus halophilus was investigated. CH2-4 CH6-2 exhibited highest 2.2 U/mL toward Glutamic-pNA. Meanwhile T. CH6-1 V7-2 showed 1.4 Leucine-pNA. total 19 compounds, including esters, alcohols, ketones, phenols, hydrocarbons sulfur containing compounds were detected in fish broth inoculated with halophilus.T. produced most 10/19 ones this study.
In this study, several factors affecting Tetragenococcus halophilus CH6-2 cell survival rate in spray drying process including growth conditions, protective agents, and stress adaption were investigated. T. CH6-2, which was grown different media, mixed with agents or at ratios of concentration to subjected before drying. The after storage determined. results showed that skim milk had the strongest effect on cells during De Man, Rogosa Sharpe (MRS) a better medium than our developed M7...
In this study, factors affecting the extraction yield of carotenoids from dry shrimp waste by organic solvents such as ratio hexane / acetone, solvent waste, temperature, time, method dynamic or static have been studied. The results showed that hexane: acetone = 3: 1 gave highest carotenoid yield. solvent’s mixture, reached at temperature 60 °C for 2 hours, which was 44,64 µg g raw (d.b.) (ratio to material 3/1). Ultrasound vortexing higher than in conditions, 1.5- 1.8- fold increase,...
The effects of different physical and technological parameter such as time, substrate to liquid ratio, enzyme concentration, temperature, pH on enzymatic saccharification alkaline pretreated straw cellulose were studied. For pretreatment, the was incubated with 10 % NaOH at ratio 1:20 (w/v) 90 °C for 1 hour. After pretreatment content increased from 50.2 (w/w) 67.3 (w/w). Enzyme used treated untreated produced Cellulosimicrobium sp MP1 which isolated termite gut. Results research showed that...