A5073063880- Nanoparticles: synthesis and applications
- Enzyme Production and Characterization
- Microbial Community Ecology and Physiology
- Probiotics and Fermented Foods
- Graphene and Nanomaterials Applications
- Pineapple and bromelain studies
- Phytase and its Applications
- Genomics and Phylogenetic Studies
- Tannin, Tannase and Anticancer Activities
- Microplastics and Plastic Pollution
- Insect Resistance and Genetics
- Enzyme Catalysis and Immobilization
- Advanced Nanomaterials in Catalysis
- Antibiotic Resistance in Bacteria
- Identification and Quantification in Food
- Gut microbiota and health
- Insect Pest Control Strategies
- Plant tissue culture and regeneration
- Insects and Parasite Interactions
- Lignin and Wood Chemistry
- Dengue and Mosquito Control Research
- Sesame and Sesamin Research
- Microbial Metabolism and Applications
- Advanced oxidation water treatment
- Studies on Chitinases and Chitosanases
National Pingtung University of Science and Technology
2014-2025
National Chung Hsing University
2004
This study was designed to investigate stressful social experience (SSE) in early life by examining how it can induce alterations the microbiota-gut-brain axis. To test this, different experimental groups of pups experienced presence either a stranger (S) with mother (M+P+S) or without their (MS+S−M). Animals were assessed for anxiety-like behavior and high-throughput bacterial 16s rRNA sequencing performed analyze structure gut microbiota. Our analysis revealed that SSE induced reduced...
Microalgal biomass offers a promising biofertilizer option due to its nutrient-rich composition, adaptability, and environmental benefits. This study evaluated the potential of microalgal-based biofertilizers-microalgal Chlorella biomass, de-oiled microalgal (DMB), de-aqueous extract (DAEMB)-in enhancing lettuce growth, soil nutrient dynamics, microbial community composition. Lettuce seedlings were cultivated with these biofertilizers, plant growth parameters, photosynthetic pigments,...
Plant probiotic bacteria are a versatile group of isolated from different environmental sources to improve plant productivity and immunity. The potential probiotic-based formulations is successfully seen as growth enhancement in economically important plants. For instance, endophytic
A cDNA fragment encoding cystatin, a cysteine protease inhibitor, was obtained from maturing sesame seeds. The clone constructed in nonfusion or fusion vector and then overexpressed Escherichia coli. recombinant cystatins were found the soluble fraction of cell extract demonstrated to be functionally active reverse zymographic assay. corresponding endogenous 22 kDa cystatin low abundance mature seeds purified homogeneity via papain-coupling affinity column confirmed by western blotting with...
The continuous requirement for pre-bleaching processes on kraft pulp, employing a range of compatible enzymes, aims to mitigate the pollution caused by chemical bleaching agents. In present study, laccase-producing bacterium Bacillus licheniformis BK-1 was isolated from Bakreshwar hot spring in India and tested laccase production using different lignocellulosic substrates. isolate found produce maximum (8.25 IU/mL) presence rice bran as substrate, followed 5.14 IU/mL sawdust over 48 h...
Textile industry effluents contain several hazardous substances, such as dye-containing effluents, which pose environmental and aesthetic challenges. Presently, the microbial-based remediation process is in use. This study investigated application of ferrous–ferric oxide (Fe3O4) nanoparticles, a readily formulated nanoadsorbent, to remove scattered dye molecules from industrial effluents. The nanoparticles were prepared using chemical co-precipitation method. had 26.93 emu g−1 magnetization,...
To evaluate silver nanoparticles’ (AgNPs) therapeutic and clinical potentials, antibacterial action, blood compatibility, antiplatelet activities are the main concerns for toxicity profiling. Heat-denatured lysozyme-mediated formulation stabilized AgNPs, thereby providing more bactericidal activity compatibility. The study of synthesis AgNPs suggests rapid cost-effective by one-step reaction using a 10:1 ratio nitrate lysozyme incubating at 60 °C two hours. Characterization was analyzed...
A cDNA fragment encoding the cysteine protease inhibitor, cystatin, was cloned from pineapple (Ananas comosus) stem. This clone constructed in a fusion vector and easily over-expressed Escherichia coli; satisfactory over-expression of non-fusion cystatin achieved after an additional start codon inserted prior to its coding sequence. Both recombinant cystatins were predominately found soluble fraction cell extract, demonstrated be functionally active reverse zymographic assay. The separately...
A method was developed for production of sesame cystatin, a thermostable cysteine protease inhibitor. Sesame cystatin first expressed in Escherichia coli as an insoluble recombinant protein fused to oleosin, unique structural seed oil bodies, by short hydrophilic linker peptide. Stable artificial bodies were constituted with triacylglycerol, phospholipid, and the oleosin−cystatin fusion protein. After centrifugation, exclusively found bodies. Proteolytic cleavage papain, effectively...
L-methioninase is an enzyme that has recently gained significant interest in the scientific community because of its potential as a targeted therapy for cancer. This study aims to isolate and identify extremophilic bacteria could produce access enzymatic isolated under stress conditions, specifically agro-industrial waste. In this study, rare marine bacterium, Alcaligenes aquatilis BJ-1, exhibited highest specific activity 4.61 U/mg at optimum pH 8.3. The was purified 4.3-fold 7.15-fold by...
Phytocystatins are natural inhibitors of cysteine protease, and may regulate endo- or exo-genous proteolytic activities in plants. They classified into Group I II differing by the presence C-terminal extension II. A cDNA fragment encoding a phytosystatin, SiCYS was previously obtained from sesame seeds.SiCYS as well its two structural domains, N-terminal domains (SiCYS-N SiCYS-C), expressed Escherichia coli. The recombinant SiCYS-N showed inhibitory activity against papain. K i values were...
Objective: The most common extraintestinal pathogen and infection site is uropathogenic Escherichia coli (UPEC), which causes urinary tract infections (UTIs). UPEC also a in bloodstream infections; severe cases, it can lead to death. Although host bacterial virulence factors have been demonstrated be associated with UTI pathogenesis, the role of related contributing source bacteremia not yet fully understood. This study aimed compare analyze patients UTI. Methods: A total 171 E. strains...
Probiotic potential of thermotolerant lactic acid bacteria isolated from "Gari "a cassava-based African fermented foodBertrand Tatsinkou Fossi, Robert Ndjouenkeu