Abstract Background Intrahepatic cholestasis of pregnancy (ICP) is characterized by pruritus and in late results adverse outcomes, including preterm delivery birth weight, which are affected the genetic environmental background. However, until now, architecture ICP has remained largely unclear. Methods Twenty-six clinical data points were recorded for 151 Chinese patients. The generated from whole-exome sequencing (WES) using BGISEQ-500 platform further analyzed Burrows-Wheeler Aligner (BWA)...

Abstract Background Intrahepatic cholestasis of pregnancy (ICP) can cause premature delivery and stillbirth. Previous studies have reported that mutations in ABC transporter genes strongly influence the transport bile salts. However, to date, their effects are still largely elusive. Methods A whole-exome sequencing (WES) approach was used detect novel variants. Rare exonic variants (minor allele frequencies: MAF < 1%) were analyzed. Three web-available tools, namely, SIFT, Mutation Taster...

Abstract Objective To investigate the value of ultrasonography in diagnosis heterotopic pregnancy and follow‐up. Methods A retrospective analysis 50 cases clinically diagnosed our hospital was performed, clinical characteristics ultrasonographic manifestations patients were summarized, reasons for initial ultrasound missed misdiagnosis analyzed, outcomes followed up. Results Among cases, diagnoses intrauterine all gestational sac type, 32 ectopic located fallopian tube, 10 uterine horn, 1...

Objective Thyroid dysfunction may play a role in the development of gestational hypertension. However, this relationship remains unclear. This study was performed to evaluate association between thyroid hormone parameters during early pregnancy and Methods Women with singleton pregnancies were recruited into prospective cohort at 9 13 weeks, their serum thyroid-stimulating hormone, free thyroxine, triiodothyronine concentrations measured using electrochemiluminescence immunoassays. In total,...

Background: Intrahepatic cholestasis of pregnancy (ICP) is associated with a high incidence fetal morbidity and mortality. Therefore, revealing the mechanisms involved in ICP its association complications very important. Methods: Here, we used whole-exome sequencing (WES) approach to detect novel mutations organic anion transporting polypeptide (OTAP) genes, ATP-binding cassette transporter (ABC) receptor genes 249 individuals 1,029 local control individuals. Two available tools, SIFT...

Preeclampsia (PE) and fetal growth restriction (FGR) have abnormal placental implantation endothelial dysfunction in common. However, their etiologies are not well understood. Both heat shock protein 70 (Hsp70) nitric oxide (NO) suggested to play a major role the regulation of maternal fetoplacental hemodynamics. In this study, association PE with FGR Hsp70 or NO was analyzed.A total 30 cases PE, 25 complicated 50 normal pregnant women were chose, animal models constructed. Subsequently,...

Abstract The current privacy protection scheme of medical electronic health records ignores the security role interaction, which leads to low record data and a long time for encryption decryption. This paper proposes method based on blockchain asymmetric encryption. uses technology algorithm establish an system model, selects alliance chain control permissions members, constructs corresponding sub key, shared users information obtains file key through verification (so as decrypt obtain plain...

Background. Placenta accreta spectrum (PAS) disorders seriously threaten the safety of mother and infant in perinatal period. Moreover, PAS is associated with poor maternal outcomes once complicated cervical implantation. Dismally, there are few reports about involvement currently, early warning models also rarely reported. To screen risk factors implantation construct an model, we performed analysis clinical indicators images patients by artificial intelligence (AI) data processing methods....

Background. Forceps delivery is one of the most important measures to facilitate vaginal delivery. It can reduce rate first cesarean Frustratingly, adverse maternal and neonatal outcomes associated with forceps have been frequently reported in recent years. There are two major reasons: that abilities doctors midwives vary from hospital other lack regulations management In order improve success incidence complications, we applied form-based under an intelligent medical model. The aim this...

Objectives: To research the associations between fructose-bisphosphate aldolase B ( ALDOB ) gene polymorphisms and intrahepatic cholestasis of pregnancy (ICP) risk. Material methods: Whole-genome sequencing (WGS) was performed to detect polymorphisms. Five web-available tools were employed predict effect site variant on protein. Protein structure comparisons reference -modified samples by SWISS-MODEL Chimera 1.14rc, respectively. Results: We identified 28 genetic variants in gene. When...

Abstract Objective To evaluate the application and effectiveness of prenatal ultrasound in diagnosing managing conjoined twins. Methods A retrospective analysis was conducted on 20 cases twins diagnosed at our hospital between January 2016 December 2022. The types twins, ultrasonographic characteristics, associated anomalies were assessed. Results gestational age diagnosis ranged from 10 to 35 weeks, with an average 14.21 ± 5.69 weeks. Thirteen detected first trimester, five early second one...

Intrahepatic cholestasis of pregnancy (ICP) is a pregnancy‐specific liver disease and the second most frequent cause jaundice in pregnancy, but etiology it poorly understood. By collecting blood fecal samples from 12 healthy pregnant women (CON group) 32 ICP patients (ICP China, we performed 16s rRNA gene sequencing analyzed microbial diversity. The results showed decrease species richness group compared to that CON group, with significant difference diversity between two groups....

Objective: To discuss the expression and meaning analysis of Hypoxia-Inducible Factor-1α (HIF-1α) Heat Shock Protein 70 (HSP70) in preeclamptic placenta.Method: Fifty patients (experimental group) with preeclampsia our hospital 50 normally pregnant women (control were recruited this study, then expressions HIF-1α HSP70 placental tissues compared between two groups.Results: Positive experimental group 80.00% (40/50) 78.00% (39/50), respectively, whereas those control 28.00% (14/50) 32.00%...

Abstract Background Data-independent acquisition (DIA) is one of the most powerful and reproducible proteomic technologies for large-scale digital qualitative quantitative research. The aim this study was to use methodologies identification biomarkers that are over- or underexpressed in women with intrahepatic cholestasis pregnancy (ICP) compared controls discover a potential biomarker panel ICP detection. Methods participants included 11 patients healthy pregnant as controls. clinical...

Abstract Background Intrahepatic cholestasis of pregnancy (ICP) can cause adverse outcomes, such as spontaneous preterm delivery and stillbirth. It is a complex disease influenced by multiple factors, including genetics the environment. Previous studies have reported that functioning nuclear receptor subfamily 1 group H member 4 ( NR1H4 ) plays an essential role in bile acid (BA) homeostasis. However, some novel variants their pathogenesis not been fully elucidated. Therefore, this research...

Background. In a global environment of increasing cesarean delivery rate, promoting vaginal delivery, reducing the rate first section, and incidence complications are objectives obstetric medical quality safety in China. As common complication, preeclampsia affects many pregnant women. It is obstetrician’s great responsibility to promote improve outcomes preeclampsia. To this end, we explored roles active labor management under smart model improving for women with Methods. The clinical data...

Objectives To identify the novel pathogenic genetic variants associated with intrahepatic cholestasis of pregnancy (ICP) disease by whole-exome sequencing (WES) approach. Design WES DNA, and conduct association between total bile acids. Setting Jiangxi. Samples 151 ICP patients. Methods DNA samples from patients were subjected to WES. Rare exonic (minor allele frequencies: MAF < 1%) performed for subsequent analysis. Main outcome measures Association other clinical disorders. Results We...

To explore the cytochrome P450 family 27 subfamily A member 1 (CYP27A1) gene mutations in Chinese women with intrahepatic cholestasis of pregnancy (ICP) and correlation between CYP27A BA (bile acid) level changes.In this study, entire coding region CYP27A1 was sequenced 151 Han ICP 1029 matched samples, pathogenicity identified judged through evolutionary conservation analysis, computational analysis protein structure modeling. Finally, we verified relationship total serum bile acid (TBA)...