A5008399704- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Probiotics and Fermented Foods
- Plant Disease Resistance and Genetics
- Fungal and yeast genetics research
- Infectious Diseases and Mycology
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Chromosomal and Genetic Variations
- Plant Pathogens and Fungal Diseases
- Enterobacteriaceae and Cronobacter Research
- Peptidase Inhibition and Analysis
- Engineering and Material Science Research
- Biochemical and Structural Characterization
- Parasitic Diseases Research and Treatment
- Nematode management and characterization studies
- Fungal Biology and Applications
University of Minnesota
2009-2025
University of Minnesota System
2024
Institut thématique Génétique, génomique et bioinformatique
2011
Candida albicans is both a member of the healthy human microbiome and major pathogen in immunocompromised individuals. Infections are typically treated with azole inhibitors ergosterol biosynthesis often leading to drug resistance. Studies clinical isolates have implicated multiple mechanisms resistance, but focused on large-scale aberrations or candidate genes, do not comprehensively chart genetic basis adaptation. Here, we leveraged next-generation sequencing analyze 43 from 11 oral...
Genetic diversity is often generated during adaptation to stress, and in eukaryotes some of this thought arise via recombination reassortment alleles meiosis. Candida albicans, the most prevalent pathogen humans, has no known meiotic cycle, yet it a heterozygous diploid that undergoes mitotic somatic growth. It been shown clinical isolates as well strains passaged once through mammalian host undergo increased levels recombination. Here, we tested hypothesis stress conditions increase rates...
Abstract The design of effective antimicrobial therapies for serious eukaryotic pathogens requires a clear understanding their highly variable genomes. To facilitate analysis copy number variations, single nucleotide polymorphisms and loss heterozygosity events in these pathogens, we developed pipeline analyzing diverse genome-scale datasets from microarray, deep sequencing, restriction site associated DNA sequence experiments clinical laboratory strains Candida albicans , the most prevalent...
In vitro studies suggest that stress may generate random standing variation and different cellular ploidy states evolve more rapidly under stress. Yet this idea has not been tested with pathogenic fungi growing within their host niche in vivo Here, we analyzed the generation of both genotypic phenotypic diversity during exposure Candida albicans to mouse oral cavity. Ploidy, aneuploidy, loss heterozygosity (LOH), recombination were determined using flow cytometry double digest restriction...
Abstract Phenotypic diversity can arise rapidly through loss of heterozygosity (LOH) or by the acquisition copy number variations (CNV) spanning whole chromosomes shorter contiguous chromosome segments. In Candida albicans, a heterozygous diploid yeast pathogen with no known meiotic cycle, homozygosis and aneuploidy alter clinical characteristics, including drug resistance. Here, we developed high-resolution microarray that simultaneously detects ∼39,000 single nucleotide polymorphism (SNP)...
Infectious diseases caused by drug-resistant fungi are an increasing threat to public health because of the high mortality rates and costs associated with treatment. Thus, understanding molecular mechanisms drug resistance is crucial interest for medical community. Here we investigated role regulated protein mistranslation, a characteristic mechanism used C. albicans diversify its proteome, in evolution fluconazole resistance. Such codon ambiguity usually considered highly deleterious, yet...
ABSTRACT Background Epidemiological surveillance of Candidozyma sp. has become important because many species this new genus have been reported to be responsible for nosocomial outbreaks and exhibit elevated minimal inhibitory concentrations (MIC) one or more classes antifungal drugs. Objectives To describe the genetic relationships among Argentinian clinical isolates belonging study molecular mechanisms associated with resistance. Methods We performed whole‐genome sequencing 41 isolates....
The early endocytic patch protein Sla2 is important for morphogenesis and growth rates in Saccharomyces cerevisiae Candida albicans, but the mechanism that connects these processes not clear. Here we report defects cells lacking Ca SLA2 or Sc are associated with a cell cycle delay influenced by Swe1, checkpoint kinase. To establish how Swe1 monitors function, compared actin organization dynamics strains other components of patches (Sla1 Abp1) those Sla2. Only sla2 had cables, known trigger...
Summary The interaction of Candida albicans with the host is a complex nature involving fungal factors and host's response. In this study, we concentrated on phenotypic expression virulence attributes genotypic characteristics C. isolates from two distinct clinical entities candidiasis—blood stream vaginal infections, possible role these factors. Hence, conducted comparative in vitro assessment characteristics, including adhesion to epithelial cells HaCat cell line, biofilm formation,...
It is unknown whether within-patient