Abstract Listeria monocytogenes is an intracellular pathogen that causes life‐threatening disease called listeriosis. However, in South Africa (SA), there a dearth of information concerning the potential role meat and products as sources for human The aim study was to determine occurrence level L. found SA characterize strains according serogroups, antimicrobial resistance profiles virulence genes. A total 2,017 samples from imported locally produced raw, processed ready eat were collected...

Consumption of food that is contaminated by microorganisms, chemicals, and toxins may lead to significant morbidity mortality, which has negative socioeconomic public health implications. Monitoring surveillance microbial diversity along the value chain a key component for hazard identification evaluation potential pathogen risks from farm consumer. The aim this study was determine in meat products different enterprises types South Africa. Samples (n = 2017) were analyzed Yersinia...

Listeria monocytogenes is an important foodborne pathogen which has the ability to adapt and survive in food processing facilities where it can persist for years. In this study, a total of 143 L. isolates South Africa (SA) were characterized their strain’s genetic relatedness, virulence profiles, stress tolerance resistance genes associated with . The Core Genome Multilocus Sequence Typing (cgMLST) analysis revealed that most frequent serogroups IVb IIa; Types (ST) ST204, ST2, ST1; Clonal...

Listeria species (spp.) are contaminants that can survive in food, on equipment, and food processing premises if appropriate hygiene measures not used. Homologous stress tolerance genes, virulence gene clusters such as the prfA cluster, of internalin genes contribute to pathogenic potential strains be carried by both nonpathogenic spp. To enhance understanding genome evolution virulence-associated properties, a comparative approach was used analyze 41 sequences belonging L. innocua...

The objective of the current study was to determine incidence contamination by top 7 Shiga toxin-producing Escherichia coli (STEC) O-groups, responsible for majority E. infections in human beings, retail meat from different animal species. Samples ground beef (n = 51), pork 16), chicken and game (deer, wild boar, bison, rabbit; n 55) were collected vendors detection STEC O-groups (O26, O45, O103, O111, O121, O145, O157). Meat samples tested using a multiplex polymerase chain reaction assay...

Salmonella enterica serotype Minnesota (S. Minnesota) has recently emerged as a predominant in Brazilian poultry farming operations. Genomic surveillance efforts concentrated primarily Europe have been used to evaluate food safety risks associated with S. imported poultry/poultry products. However, the burden imposed by on consumers sub-Saharan Africa is not understood. Here, we whole-genome sequencing (WGS) characterize 36 strains from raw into South Africa, specifically: (i) 11 isolated at...

Abstract Zoonoses are a worldwide public health concern, accounting for approximately 75% of human infectious diseases. In addition, zoonoses adversely affect agricultural production and wildlife. We review some mathematical models developed the study viral in wildlife identify areas where further modeling efforts needed.

Zoonoses, which account for approximately 75% of emerging human infectious diseases worldwide, pose a re-emerging threat to public health. With an ever-increasing interrelationship between humans, livestock and wildlife species, the health will rise unprecedented levels. Wildlife species contribute majority diseases; therefore, there is urgent need define control systems zoonoses origin but very little information exists. In this review, we examine prevalent zoonotic infections reported in...

Processed meat is a target in adulteration for economic gain. This study demonstrates molecular and bioinformatics diagnostic pipeline, utilizing the mitochondrial 16S ribosomal RNA (rRNA) gene, to determine processed product mislabeling through Next-Generation Sequencing. Nine pure samples were collected artificially mixed at different ratios verify specificity sensitivity of pipeline. products (n = 155), namely, minced meat, biltong, burger patties, sausages, across South Africa....

Meat products have been implicated in many listeriosis outbreaks globally, however there is a dearth of information on the diversity L. monocytogenes isolates circulating food South Africa. The aim this study was to investigate population structure isolated meat value chain within African market. Based whole-genome sequence analysis, total 217 were classified into two main lineage groupings namely lineages I (n = 97; 44.7%) and II 120; 55.3%). groups further differentiated IIa 95, 43.8%),...

A confirmed case of human brucellosis motivated an investigation into the potential source infection in Namibia. Since domestic animals are principal sources Brucella humans, 1692 serum samples were screened from sheep, goats and cattle 4 presumably at-risk farms 900 springbok (Antidorcas marsupialis) 29 mixed farming units for antibodies by Rose-Bengal test (RBT) positive cases complement fixation (CFT). To assess prevalence brucellosis, 137 abattoir employees tested using standard tube...

Listeria monocytogenes is a highly fatal foodborne causative agent that has been implicated in numerous outbreaks and related deaths of listeriosis the world. In this study, six L. isolated from ready-to-eat (RTE) meat products were analysed using Whole Genome Sequencing (WGS) to identify virulence resistance genes, prophage sequences, PCR-serogroups, sequence types (STs). The WGS identified four different STs (ST1, ST121, ST204, ST876) belonged serogroup 4b (lineage I) 1/2a II). Core...

Introduction Macrococcus species have been isolated from a range of mammals and mammal-derived food products. While they are largely considered to be animal commensals, spp. can opportunistic pathogens in both veterinary human clinical settings. This study aimed provide insight into the evolution, population structure, functional potential genus, with an emphasis on antimicrobial resistance (AMR) virulence potential. Methods All high-quality, publicly available genomes ( n = 104, accessed 27...

The objective of the study was to assess pH measurements between offal organs different species and association taken 4 h post-slaughter predictor variables in liver lungs. A linear regression analysis conducted on selected identify main predictors their interactions affecting meat post-slaughter. In an increasing order magnitude during winter, achieved at 16 - 36 springbok heart, liver, spleen, kidney lungs significantly (p < 0.05) higher than 6.0. attained carcasses below 6.0, whilst no...

Staphylococcus aureus, including methicillin-resistant strains, has been detected in food products of animal origin globally. Limited data have reported on the factors contributing to antibiotic resistance food-borne pathogens South Africa. The primary aim this study was determine prevalence S. antibiotic-resistant poultry meat as well evaluation potential risk for contamination with aureus isolates. A cross-sectional investigation conducted municipalities located across nine provinces...

Annually, approximately 23,000 cases of food poisoning by Staphylococcus aureus enterotoxins are reported worldwide. The aim this study was to determine the occurrence and characterize S. on beef products in South Africa. Organ meats (n = 169), raw processed meat 110), intact 53), ready-to-eat 68) were obtained from 25 retail outlets. isolated enumerated according ISO 6888-1 method. Identification strains performed MALDI-TOF MS. antimicrobial resistance determined using disc diffusion test....

Consumption of food that is contaminated with Shiga toxin-producing

Yersinia enterocolitica infections impose a significant public health and socioeconomic burden on human population in many countries. The current study investigated the prevalence, antimicrobial resistance profile molecular diversity of Y. meat products across various retail outlets selected provinces South Africa (SA). In cross-sectional study, total 581 were collected from four cities three SA. Samples beef pork products, which included 292 raw intact, 167 processed, 122 ready-to-eat (RTE)...

Salmonellosis and antimicrobial resistance caused by non-typhoidal Salmonella are public health concerns. This study aimed at determining prevalence, serovars, virulence factors of from beef products. Four-hundred samples 25 retail outlets in KwaZulu-Natal, South Africa were analyzed for using standard methods, confirmation with matrix-assisted laser desorption ionization–time flight serotyping according to the White–Kauffmann–Le Minor scheme. The Kirby Bauer disk diffusion method was used...

Escherichia coli is found in diverse environmental niches, including meat and products, known for its significance both food safety public health. In South Africa, whole genomic sequencing (WGS) efforts E. are overwhelmingly skewed toward human isolates with limited studies conducted on non-human isolates. Therefore, the aim of this study was to use WGS characterise generic strains isolated from animal specimens, meat, meat-based products Africa. Based analysis, a total 35 were grouped into...

The aims of the study were to assess heavy metals (lead and cadmium) values in meat offal from harvested springbok. For cadmium (Cd), mean concentrations liver kidney 0.10 ± 0.04 mg/kg, 0.33 0.05 mg/kg 1.04 0.15 0.91 0.12 for lead (Pb). None samples had a Cd level above limits set (0.5 mg/kg) 1.0 while two Pb 12 mg/kg). muscles below detection limit. In current study, interpretation results was limited by small number positive samples; however, within range previously reported literature...

Outbreaks of Rift Valley fever (RVF) occurred in Namibia 2010 and 2011. Complete genome characterization was obtained from virus isolates collected during disease outbreaks southern wildlife Etosha National Park 2011, close to the area where RVF domestic livestock. The strains were sequenced using Sanger sequencing (Namibia_2010) or next generation (Namibia_2011). A sequence-independent, single-primer amplification (SISPA) protocol used combination with Illumina Next 500 sequencer....