Pancreatic K ATP channel trafficking defects underlie congenital hyperinsulinism (CHI) cases unresponsive to the opener diazoxide, mainstay medical therapy for CHI. Current clinically used inhibitors have been shown act as pharmacochaperones and restore surface expression of mutants; however, their therapeutic utility impaired CHI is hindered by high-affinity binding, which limits functional recovery rescued channels. Recent structural studies channels employing cryo-electron microscopy...

Pancreatic K ATP channel trafficking defects underlie congenital hyperinsulinism (CHI) cases unresponsive to the opener diazoxide, mainstay medical therapy for CHI. Current clinically used inhibitors have been shown act as pharmacochaperones and restore surface expression of mutants; however, their therapeutic utility trafficking-impaired CHI is hindered by high affinity binding, which limits functional recovery rescued channels. Recent structural studies channels employing cryo-electron...

HAdV-7 is a prevalent pathogen that can cause severe pneumonia in children. Previous studies have shown significant increase serum levels of vascular permeability factor (VPF/VEGF) and viral load pediatric patients with fatal infection, suggesting potential damage to the pulmonary endothelium. Further research necessary elucidate underlying mechanism. The human lung microvascular endothelial cell line-5a CD46 mice were used for vitro vivo experiments, respectively. RNA-seq was employed...

Human bocavirus 1 (HBoV1) belongs to the family Parvoviridae and it is acknowledged that HBoV1 a respiratory pathogen. We report case of 13-month-old boy who presented with cough, shortness breath, wheezing, eventually died severe pneumonia acute distress syndrome (ARDS). Metagenomics next-generation sequencing (mNGS) showed was only detected The nasopharyngeal aspirate viral load 2.08 × 1010 copies/ml serum 2.37 105 copies/ml. child still oxygen deficient under mechanical ventilation. Chest...

Stimulation of unmyelinated C fibers, the nociceptive sensory nerves, by noxious stimuli is able to initiate host responses. Host defensive responses against respiratory syncytial virus (RSV) infection rely on induction a robust alpha/beta interferon (IFN-α/β) response, which acts restrict viral production and promote antiviral immune Alveolar macrophages (AMs) are major source IFN-α/β upon RSV infection. Here, we found that fibers involved in defense Compared control mice post-RSV...

Respiratory syncytial virus (RSV) a leading cause of pediatric and adult morbidity mortality worldwide. It can complications in multiple organs, thus increasing hospital stays costs. However, RSV-based studies have primarily focused on effects the lungs blood, thereby potentially neglecting critical genes pathways. Hence, studying RSV infection via novel multi-organ approach is important. In this study, lung, intestine, brain, spleen tissues from six BALB/c mice (6–8 weeks old; three control...

<title>Abstract</title> <bold>Background</bold> HAdV-7 is a prevalent pathogen that can cause severe pneumonia in children. Previous clinical studies have shown significant increase serum levels of vascular endothelial growth factor (VEGF) and viral load pediatric patients with fatal infection, suggesting potential damage to pulmonary cells increased permeability. Further research necessary elucidate the underlying mechanism behind this finding. <bold>Methods</bold> The human lung...

Pancreatic KATP channel trafficking defects underlie congenital hyperinsulinism (CHI) cases unresponsive to the opener diazoxide, mainstay medical therapy for CHI. Current clinically used inhibitors have been shown act as pharmacochaperones and restore surface expression of mutants; however, their therapeutic utility impaired CHI is hindered by high-affinity binding, which limits functional recovery rescued channels. Recent structural studies channels employing cryo-electron microscopy...

Pancreatic KATP channel trafficking defects underlie congenital hyperinsulinism (CHI) cases unresponsive to the opener diazoxide, mainstay medical therapy for CHI. Current clinically used inhibitors have been shown act as pharmacochaperones and restore surface expression of mutants; however, their therapeutic utility trafficking-impaired CHI is hindered by high affinity binding, which limits functional recovery rescued channels. Recent structural studies channels employing cryo-electron...

Pancreatic K ATP channel trafficking defects underlie congenital hyperinsulinism (CHI) cases unresponsive to the opener diazoxide, mainstay medical therapy for CHI. Current clinically used inhibitors have been shown act as pharmacochaperones and restore surface expression of mutants; however, their therapeutic utility impaired CHI is hindered by high-affinity binding, which limits functional recovery rescued channels. Recent structural studies channels employing cryo-electron microscopy...

To investigate the effect of silencing CD46 and desmoglein 2 (DSG2) in host A549 cells on entry human adenovirus type 3 (HAdV-3) 7 (HAdV-7) cell secretion inflammatory cytokines.RNA interference technique was use to silence expression or DSG2 epithelial alveolar as HAdV-3 HAdV-7. The binding viruses with were observed immunofluorescence staining at 0.5 1 h after viral infection. load determined qRT-PCR, IL-8 level measured using ELISA.In infected cells, immunofluorescent revealed...

Human adenovirus type 7 (HAdV-7) can cause severe pneumonia and complications in children. However, the mechanism of pathogenesis genes involved remain largely unknown. We collected HAdV-7-infected mock-infected A549 cells at 24, 48, 72 hours postinfection (hpi) for RNA sequencing (RNA-Seq) identified potential functional pathways associated with HAdV-7 infection using weighted gene coexpression network analysis (WGCNA). Based on bioinformatics analysis, 12 modules were constructed by WGCNA,...

Abstract As nociceptors, C-fibers play a critical role in maintaining host homeostasis under both physiological and pathological conditions. We previously demonstrated that C-fiber degeneration confers protection against respiratory syncytial virus (RSV) infection. However, comprehensive investigation on the effects of state remains unexplored. To address this gap, we established C-fiber-degenerated (KCF) BALB/c mouse model validated it by immunofluorescence staining multiple organs....

Abstract Background Human adenovirus type 7 (HAdV-7) can cause severe pneumonia in children and complications. At present, however, the pathogenesis genes involved remain largely unknown. Methods We collected HAdV-7 infected mock-infected A549 cells at 24, 48, 72 hours post-infection (hpi) for RNA sequencing (RNA-seq). And, we identified potential functional pathways associated with infection using weighted gene co-expression network analysis (WGCNA). Results Based on bioinformatics...

Abstract Human adenovirus type 7 (HAdV-7) can cause severe pneumonia and complications in children. However, the pathogenesis genes involved remain largely unknown. We collected HAdV-7 infected mock-infected A549 cells at 24, 48, 72 hours post-infection (hpi) for RNA sequencing (RNA-seq), identified potential functional pathways associated with infection using weighted gene co-expression network analysis (WGCNA).Based on bioinformatics analysis, 12 modules were constructed by WGCNA, blue,...