A5007297883- Diabetes and associated disorders
- Pancreatic function and diabetes
- Thyroid Cancer Diagnosis and Treatment
- Head and Neck Anomalies
- Diabetes Management and Research
- Thyroid Disorders and Treatments
- Immune Cell Function and Interaction
- Reproductive Biology and Fertility
- Cancer Immunotherapy and Biomarkers
- Reproductive Physiology in Livestock
- Polyamine Metabolism and Applications
- Sperm and Testicular Function
- Thyroid and Parathyroid Surgery
- T-cell and B-cell Immunology
- Genetic and phenotypic traits in livestock
- Vitamin D Research Studies
- Melanoma and MAPK Pathways
- BRCA gene mutations in cancer
- Selenium in Biological Systems
- Male Reproductive Health Studies
- Genomics and Rare Diseases
- Animal Nutrition and Physiology
- Cell death mechanisms and regulation
- Andrographolide Research and Applications
- Salivary Gland Disorders and Functions
University of Toledo
2016-2025
University of Toledo Medical Center
2019-2023
Ohio University
2019-2023
ProMedica Russell J. Ebeid Children's Hospital
2020
Diponegoro University
2015
University of Wisconsin–Madison
2012-2014
Indian Council of Agricultural Research
2008
Indian Veterinary Research Institute
2008
Background: Hashimoto's thyroiditis (HT) has been found to coexist with differentiated thyroid cancer (DTC) in surgical specimens, but an association between the two conditions discounted by medical literature. Therefore, we performed this study determine any potential relationship HT and risk of developing DTC. Methods: We collected data for thyrotropin (TSH), thyroxine (T4), peroxidase antibody (TPO-Ab) titers, pathology, weight-based levothyroxine (LT4) replacement dose patients who were...
<h3>Background</h3> Thyroid cancer and thyroid autoimmunity are considered opposite extremes of immune-responses. However, several studies have suggested that coexists with autoimmune diseases like Hashimoto Thyroiditis (HT) Graves disease (GD). We shown the risk developing is higher in patients a silent form -Euthyroid Thyroiditis-(EHT). <h3>Methods</h3> analyzed data from 2633 consecutive GD, HT, EHT non-Autoimmune Disease (Non-AITD) for presence Differentiated Cancer (DTC). further...
Thyroid cancers are usually surrounded by a significant number of immune-reactive cells. Tumor-associated lymphocytes as well background lymphocytic thyroiditis frequently mentioned in pathology reports patients who have undergone surgery for thyroid cancer. The nature this reaction is not understood. fact that cancer can survive adverse microenvironment indicative immune regulation. We characterized the infiltration accompanies and compared it with present autoimmunity. found...
Progesterone and luteal blood flow effects of an i.u. 2-h infusion 0.25 mg/h prostaglandin F(2)(alpha) (PGF) that simulated a natural pulse 13,14-dihydro-15-keto-PGF (PGFM) were compared to the single bolus injection PGF (4 mg) induced complete luteolysis in heifers. Blood sampling estimate percentage area with colour-Doppler signals performed every 2 min for 20 less frequently thereafter 6 h. After beginning or injection, progesterone increased peak at 14 10 respectively, was accompanied by...
Background: Incidental finding of differentiated thyroid microcarcinomas (DTMc) in patients undergoing surgery for benign indications has become increasingly common. Even though carcinogenesis might relate to the background disease gland, incidence DTMc setting various disorders remains unclear. We designed present study address this question. Materials and Methods: reviewed data from two prospectively collected databases consecutive high-volume tertiary care referral centers, one United...
The generation of functionally active, stable T regulatory cells (Tregs) is a crucial target type 1 diabetes (T1D) immunotherapy. This study investigated therapeutic intervention for T1D/Latent autoimmune in adults (LADA), wherein the diabetogenic proinflammatory Treg (intermediate) cell subset was characterized and driven to phenotype (CD4+CD25+FOXP3+). involved simultaneous inhibition eukaryotic initiation factor 5a (eIF5a) Notch pathways using GC7 (N1-Guanyl-1,7-diaminoheptane) Anti-DLL4...
Abstract We have developed a transgenic mouse model of Type 1 Diabetes (T1D) in which human GAD65 is expressed pancreatic β-cells, and MHC-II on antigen presenting cells. Induced presentation activates T-cells, initiates the downstream events leading to diabetes. In our humanized mice, we shown downregulation eukaryotic translation initiation factor 5 A (elF5A), only actively dividing mammalian In-vivo inhibition elF5A hypusination by deoxyhypusine synthase (DHS) inhibitor “GC7” was studied;...
Background Thyroid nodules are an extremely common entity, and surgery is considered the ultimate diagnostic strategy in those with unclear malignant potential. Unfortunately, strategies aiming to predict risk of malignancy have inadequate specificity. Our group recently found that microenvironment thyroid cancer characterized by enhanced immune invasion activated response mediated double-negative T lymphocytes (DN T) (CD3 + CD4 - CD8 ), which believed enable or promote tumorigenesis. In...
Therapeutic options for treatment of type 1 diabetes (T1D) are still missing. New avenues immune modulation need to be developed. Here we attempted at altering the outcome our humanized model T1D by inhibiting translation-initiation factor eIF5A hypusination in vivo. Double-transgenic (DQ8-GAD65) mice were immunized with adenoviral vectors carrying GAD65 induction. Animals subsequently treated deoxyhypusine synthase (DHS) inhibitor GC7 and monitored development over time. On one hand, helper...
Regulatory T (Treg) cells play a critical role in maintenance of tolerance and are ideal for development therapies designed to suppress inflammation autoimmunity. Emerging data indicate that, compared polyclonal Tregs, adoptive transfer disease-relevant antigen-specific Tregs is advantageous terms reduced risk nonspecific immune suppression need fewer cells. The successful use chimeric antigen receptor (CARs) technology the generation effector suggests that similar approach can be used...
Objective: Nivolumab, anti–programmed cell death 1 monoclonal antibody, is a newly Food and Drug Administration–approved biologic for the treatment of metastatic melanoma. New indications other cancers have been recently granted. Nivolumab package insert reads: "Warnings Precautions: immune-mediated pneumonitis, colitis, hepatitis, nephritis renal dysfunction, hypothyroidism hyperthyroidism may occur." Development type diabetes as an adverse event was not documented in England Journal...
For some, chronic lymphocytic thyroiditis (Hashimoto thyroiditis) is an important risk factor for differentiated thyroid cancer (DTC). Surgical cohort studies even suggested a potential role peroxidase antibodies (TPO-Abs) on that risk.
Abstract Background Higher-but-within-normal thyrotropin (thyroid-stimulating hormone, TSH) is associated with higher risk for differentiated thyroid cancer (DTC) in surgical series. Our recent clinical observations suggest that this not the case presence of autoimmune disease (AITD). We designed present study to clarify controversy. Methods analyzed our prospectively collected database patients referred surgery at 2 tertiary care referral centers Greece and United States. data preoperative...
Type 1 diabetes (T1D) results from the destruction of pancreatic β-cells caused by an altered immune balance in microenvironment. In humans as well mouse models, T cells are recognized key orchestrators T1D, which is characterized helper (Th) and Th17 cell bias and/or low/defective T-regulatory (Treg), culminates cytotoxic T-cell (CTL)-mediated β-cells. Refitting toward non-inflammatory phenotype pancreas may represent a way to prevent/treat T1D. Recently we developed unique spontaneous...
The present study was undertaken to elucidate the effect of non-luteal oviductal proteins on sperm characteristics in Murrah buffaloes. Oviducts from healthy buffaloes were collected immediately after slaughter and oestrous cycle phase determined as either luteal or based ovarian morphology. Non-luteal oviducts (n = 80) flushed isthmic end oviduct with PBS, fluid centrifuged at 10,000 g 4 degrees C for 20 min then dialysed clarified. supernatant obtained lyophilized concentrate protein...
Thyroid nodules' size should not be the sole criterion for thyroidectomy; however, many patients undergo surgery large or slowly growing nodules.We evaluated risk clinically significant thyroid cancer in with reviewed data from 2 prospectively collected databases of undergoing thyroidectomies tertiary referral centers USA and Greece over 14 consecutive years. We on preoperative surgical indication, FNA cytology, pathology. included subjects operated solely nodules, without any known presumed...