A5013617927- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Heterotopic Ossification and Related Conditions
- Eosinophilic Disorders and Syndromes
- Gut microbiota and health
- Colorectal Cancer Screening and Detection
- Kruppel-like factors research
- Tryptophan and brain disorders
- Ginseng Biological Effects and Applications
- Acute Myeloid Leukemia Research
- Pneumocystis jirovecii pneumonia detection and treatment
- Radiomics and Machine Learning in Medical Imaging
- Chronic Myeloid Leukemia Treatments
- Alzheimer's disease research and treatments
- Genomics and Phylogenetic Studies
- Antifungal resistance and susceptibility
- Bacteriophages and microbial interactions
- Molecular Biology Techniques and Applications
- Agronomic Practices and Intercropping Systems
- Mycobacterium research and diagnosis
- Machine Learning in Healthcare
- Cancer Genomics and Diagnostics
- Renal Transplantation Outcomes and Treatments
- Urinary Tract Infections Management
- Legume Nitrogen Fixing Symbiosis
- Plant Parasitism and Resistance
University of California, Irvine
2020-2024
University of California, Riverside
2024
Recent reports implicate gut microbiome dysbiosis in the onset and progression of Alzheimer's disease (AD), yet studies involving model animals overwhelmingly omit microbial perspective. Here, we evaluate longitudinal microbiomes metabolomes from a popular transgenic mouse for familial AD (5xfAD). Cecal fecal samples 5xfAD wild-type B6J (WT) mice 4 to 18 months age were subjected shotgun Illumina sequencing. Metabolomics was performed on plasma feces subset same animals. Significant...
Abstract INTRODUCTION Emerging evidence links changes in the gut microbiome to late‐onset Alzheimer's disease (LOAD), necessitating examination of AD mouse models with consideration microbiome. METHODS We used shotgun metagenomics and untargeted metabolomics study human amyloid beta knock‐in (hAβ‐KI) murine model for LOAD compared both wild‐type (WT) mice a early‐onset (3xTg‐AD). RESULTS Eighteen‐month female (but not male) hAβ‐KI microbiomes were distinct from WT microbiomes, genotype...
Colorectal cancer is the second most deadly and third common in world. Its development heterogenous, with multiple mechanisms of carcinogenesis. Two distinct include adenoma-carcinoma sequence serrated pathway. The gut microbiome has been identified as a key player sequence, but its role carcinogenesis less clear. In this study, we characterized 140 polyp-free polyp-bearing individuals using colon mucosa fecal samples to determine if composition was associated each two pathways. We...
Chronic inflammation is integral to myeloproliferative neoplasm (MPN) pathogenesis. JAK inhibitors reduce cytokine levels, but not without significant side effects. Nutrition a low-risk approach and ameliorate symptoms in MPN. We performed randomized, parallel-arm study determine the feasibility of an education-focused Mediterranean diet intervention among patients with
ABSTRACT Tamoxifen is the most prescribed drug used to prevent breast cancer recurrence, but patients show variable responses tamoxifen. Such differential inter-individual response has a significant socioeconomic impact as one in eight women will develop and nearly half million people United States are treated with tamoxifen annually. orally delivered must be activated by metabolizing enzymes liver; however, clinical studies that neither genotype nor hepatic metabolic sufficient predict why...
The capacity of the human microbiome to modulate inflammation in context cancer is becoming increasingly clear. Myeloproliferative neoplasms (MPNs) are chronic hematologic malignancies which plays a key role disease initiation, progression, and symptomatology. To better understand composition gut patients with MPN, triplicate fecal samples were collected from 25 MPN non-MPN controls. Although most variance between microbial community compositions could be attributed individual (permutational...
The gut microbiome serves as an interface between the host and diet. Diet both play important roles in managing inflammation, which is a key aspect of myeloproliferative neoplasm (MPN). Studies have shown that Mediterranean (MED) diet can reduce inflammation. Therefore, we longitudinally characterized microbiomes MPN patients response to or standard 2020 US Guidelines for Americans dietary counseling determine whether there were microbiome-associated changes We did not find significant...
Adapted from: https://www.protocols.io/view/low-volume-methodology-for-nextera-dna-flex-librar-be6rjhd6 and Julio Avelar-Barragan protocol from Katrine Whiteson's lab Last modified: May 6th, 2021 Objective: To prepare a DNA sequencing library for Illumina sequencing. This can be done over two days, with stopping point after PCR amplification. presents low volume methodology the Prep Kit (96 Samples). method increases number of libraries which generated using each kit 384 samples (4 x...
ABSTRACT Purpose Chronic inflammation is integral to Myeloproliferative Neoplasm (MPN) pathogenesis. JAK inhibitors reduce cytokine levels, but not without significant side effects. Nutrition a low-risk approach and ameliorate symptoms in MPN. We performed randomized, parallel-arm study determine the feasibility of an education-focused Mediterranean diet intervention among MPN patients. Experimental Design randomly assigned participants either or standard US Dietary Guidelines for Americans...
Abstract Myeloproliferative neoplasms (MPN) are a class of hematological malignancies which result in the overproduction myeloid lineage cells. These increased cytokine production and inflammation, correlate with worsened symptom burden prognosis. Other than bone marrow transplantation, there is no cure for myeloproliferative neoplasms. As such, treatments focus on reducing thrombotic risk, burden. Because current pharmacological carry significant side-effects, need to explore low-risk...
Abstract INTRODUCTION Microbial exposures impact Alzheimer’s disease (AD), and a baseline understanding of AD model microbiomes is vital for improving efficacy. We describe the evaluation microbiome metabolome in longitudinal samples from 5xfAD transgenic mouse model. METHODS Cecal fecal wild-type B6J (WT) animals 4– 18 months age were subjected to DNA extraction shotgun Illumina sequencing. Metabolomics was performed on plasma feces subset animals. RESULTS Significant sex, age,...
Plant hosts can gain significant growth benefits from symbiosis with microbes, but these could be threatened by divergent fitness interests among partners. Here, we measured outcomes in symbiosis, varying the genotypes of both microbes and hosts, to examine scenarios that might favor uncooperative symbionts. We studied associations between Acmispon strigosus, an annual legume native California, its nitrogen fixing symbionts genus Bradyrhizobium. Bradyrhizobium form root nodules on compatible...
This protocol presents a low volume methodology for the Nextera DNA Flex Library Prep Kit (96 Samples). method increases number of sequencing libraries which can be generated using each kit from 384 samples (4 x 96-wells) to 864 (9 96-wells). While other low-cost methods have been deveolped this purpose, is very straightforward utilizing reduced reagent and sample volumes. Update: The "Nextera Prep" has renamed "Illumina with appropriate indexes having similarly renamed. all reagents steps...
<p>Patients with MPN can adopt a Mediterranean eating pattern dietician counseling and education. <b>A,</b> Percentage of participants MEDAS scores ≥8 at each timepoint orange shaded area depicting the active intervention period. <b>B,</b> Participant responses to feasibility question during period, green represents participant who met benchmark HEI-2015 (<b>C</b>) was calculated from 24-hour diet recall, for were averaged pre-intervention (weeks...
<p>Supplementary Figure 3. Changes in JAK2V617F allele burden over time.</p>
<p>Supplementary Figure 1. Complete blood count (CBC) data from participants.</p>
<p>Baseline and week 9 BMI. Baseline BMI was calculated using height weight at enrollment, with no significant differences between USDA MED groups. Weight followed throughout the study, changes in were observed either group.</p>
<p>Supplementary Figure 2. Changes in hsCRP during the study</p>
<p>Surveys used in the study</p>
<p>Energy intake, fiber and percentage of calories from carbohydrates, sugar, protein, fat calculated 24-hour diet recalls. Data are represented as mean ± SD</p>