A5024927787- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Immune Response and Inflammation
- Pharmacological Receptor Mechanisms and Effects
- Stress Responses and Cortisol
- Escherichia coli research studies
- Cytokine Signaling Pathways and Interactions
- Immune Cell Function and Interaction
- Chemical Synthesis and Analysis
- T-cell and B-cell Immunology
- Biochemical effects in animals
- Antimicrobial Peptides and Activities
- Monoclonal and Polyclonal Antibodies Research
- Lipid Membrane Structure and Behavior
- Influenza Virus Research Studies
- Yersinia bacterium, plague, ectoparasites research
- Adipose Tissue and Metabolism
- Pancreatic function and diabetes
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Regulation of Appetite and Obesity
- Pharmacological Effects of Natural Compounds
- Erythrocyte Function and Pathophysiology
- Hemoglobin structure and function
- Bacterial Genetics and Biotechnology
- Pain Mechanisms and Treatments
Institute of Bioorganic Chemistry
2013-2023
Ministry of Health of the Russian Federation
2023
Institute of Bioorganic Chemistry
2023
Russian Academy of Sciences
2003-2014
Institute of Theoretical and Experimental Biophysics
2002-2005
Institute for Theoretical and Experimental Physics
2002
Development Agency of Serbia
1999
Institute of Immunological Engineering
1991-1998
Institute of Immunology
1997-1998
Institute of Engineering
1997-1998
Understanding the interaction of Yersinia pestis with key components immune system is important for elucidation pathogenesis bubonic plague, one most severe and acute bacterial diseases. Here we report specific, high affinity binding ( K d = 1.40 × 10 −10 M ± 0.14 ) radiolabelled human interleukin 1β (hIL‐1β) to E . coli cells carrying capsular fl operon Y. pestis. Caf1A outer membrane usher protein was isolated greater than 98% purity. Competition studies purified Caf1, together...
Abstract Selective agonist of nonopioid β‐endorphin receptor decapeptide immunorphin (SLTCLVKGFY) was labeled with tritium (the specific activity 24 Ci/mmol). [ 3 H]Immunorphin found to bind mouse peritoneal macrophages ( K d = 2.0 ± 0.1 n M ). The H]immunorphin binding inhibited by unlabeled i 2.9 0.2 ) and not naloxone, α‐endorphin, γ‐endorphin [Met 5 ]enkephalin > 10 µ Thirty fragments have been synthesized their ability inhibit the studied. Unlabeled fragment 12–19 (TPLVTLFK, author's...
The octapeptide corresponding to human interferon‐α 2 (Hu IFN‐α ) sequence 131–138 hu??? high affinity murine thymocyte receptors ( K 6 = 4.2 × 10 −11 M, about 700 per cell). peptide/receptor binding is inhibited by both Hu rl FN‐α 1 8.6 −10 M) and thymosin‐α (TM‐α 3 −??? as well the homologous TMα‐α 16–23 4.5 −1 M). peptide from (131–138) activates blast transformation at a concentration of M in presence 2.5 μ/ml concanavalin A.
Two selective agonists of nonopioid β ‐endorphin receptor, synthetic peptides TPLVTLFK (octarphin) and SLTCLVKGFY (immunorphin), were labeled with tritium to specific activity 29 25 Ci/mmol, respectively. Both found bind high‐affinity naloxone‐insensitive binding sites on the membranes isolated from rat myocardium (Kd = 2.0 ± 0.2 2.5 0.3 nM, respectively). The [ 3 H]octarphin myocardial was inhibited by unlabeled (Ki 1.9 nM) immunorphin 2.2 nM). H]immunorphin 2.3 octarphin 2.4 specificity...
Abstract The synthetic peptide TPLVTLFK corresponding to the sequence 12–19 of β‐endorphin (referred as octarphin) was found bind high‐affinity naloxone‐insensitive binding sites on membranes isolated from rat brain cortex ( K d = 2.6 ± 0.2 n M ). specificity study revealed that these were insensitive not only naloxone but also α‐endorphin, γ‐endorphin, [Met 5 ]enkephalin, and [Leu well. [ 3 H]octarphin specific with inhibited by unlabeled i 2.4 ) a selective agonist nonopioid receptor...