A5001999488- RNA modifications and cancer
- Oral and Maxillofacial Pathology
- Peptidase Inhibition and Analysis
- Salivary Gland Tumors Diagnosis and Treatment
- Head and Neck Cancer Studies
- Cancer-related gene regulation
- Tumors and Oncological Cases
- Cancer therapeutics and mechanisms
- Oral Health Pathology and Treatment
- Gastric Cancer Management and Outcomes
- Diet and metabolism studies
- Esophageal and GI Pathology
- Lung Cancer Research Studies
- Liver Disease Diagnosis and Treatment
- Bone Tumor Diagnosis and Treatments
- Diet, Metabolism, and Disease
- Salivary Gland Disorders and Functions
- Cancer Treatment and Pharmacology
- Oral health in cancer treatment
- Vascular Malformations and Hemangiomas
- Nitric Oxide and Endothelin Effects
- Gastroesophageal reflux and treatments
- Ear and Head Tumors
- Bone health and treatments
- Renin-Angiotensin System Studies
Nippon Medical School
2020-2024
Tokyo Dental College
2014-2023
Nippon Dental University
2022
Nippon Medical School Hospital
2021
Teikyo University
2016-2020
Chiba Hokusou Hospital
2020
Nippon Kayaku (Japan)
1999-2017
Red Cross Hospital
2002
Yokohama City University
2001
University of Shizuoka
1990-1996
Abstract 7-Ethyl-10-hydroxy-camptothecin (SN-38), a biological active metabolite of irinotecan hydrochloride (CPT-11), has potent antitumor activity but not been used clinically because it is water-insoluble drug. For delivery by i.v. injection, we have successfully developed NK012, SN-38-releasing nanodevice. The purpose this study to investigate the pharmacologic character NK012 as an anticancer agent, especially in vascular endothelial growth factor (VEGF)–secreting tumor model. particle...
The structure and function of chromatin can be altered by modifications to histone. Histone acetylation is a reversible process governed histone acetyltransferases deacetylases (HDACs). HDAC6 subtype the HDAC family that deacetylates alpha-tubulin increases cell motility. We investigated expression levels mRNA protein in oral squamous carcinoma (OSCC)-derived lines human primary OSCCs elucidate potential involvement OSCC. Using quantitative real-time reverse transcription polymerase chain...
In the oral and maxillofacial surgery dentistry fields, use of three-dimensional (3D) patient-specific organ models is increasing, which has increased cost obtaining them. We developed an environment in our facility we can design, fabricate, 3D called "One-stop printing lab". The lab made it possible to quickly inexpensively produce that are indispensable for surgery. report model fabrication after determining dimensional accuracy with different laminating pitches (; layer thickness)...
Crystallographic studies have elucidated the binding mechanism of forskolin and P-site inhibitors to adenylyl cyclase. Accordingly, computer-assisted drug design has enabled us identify isoform-selective regulators After examining more than 200 newly synthesized derivatives forskolin, we found that modification at positions C6 C7, in general, enhances isoform selectivity. The 6-(3-dimethylaminopropionyl) led an enhanced selectivity for type V, whereas 6-[N-(2-isothiocyanatoethyl)...
To present the results of clinical study carbon ion radiotherapy (CIRT) for skull base and paracervical spine tumors at National Institute Radiological Sciences in Chiba, Japan.The is comprised three protocols: a pilot study, phase I/II dose escalation II study. All patients were treated by 16 fractions 4 weeks with total doses 48.0, 52.8, 57.6, 60.8 Gy equivalents (GyE).As result CIRT tumors, fractionation GyE/16 was decided as recommended because acceptable normal tissue reactions good...
Abstract Background Non-alcoholic fatty liver disease (NAFLD) is a hepatic manifestation of metabolic syndrome. Within the spectrum NAFLD, non-alcoholic steatohepatitis (NASH) in combination with inflammation and fibrosis can lead to cirrhosis hepatocellular carcinoma. Dysbiosis was reported contribute NASH pathogenesis. This study aimed determine effects fructo-oligosaccharides (FOS) on visceral adiposity an obese mouse model NASH. Methods Twelve newborn C57BL/6 J male mice were...
Three-dimensional (3D) printing technology in medicine is one of the new and innovative for fabricating 3D models complex anatomical structures that can be observed both visually haptically. Patient-specific fabricated through this process are currently being used various purposes, including surgical simulation, training, medical education. Most personal use/low-end desktop printers becoming widespread fused deposition modeling (FDM) printers. Compared to professional/high-end printers,...
Abstract Background It is unclear whether prophylactic endoscopic closure after colorectal submucosal dissection (ESD) reduces the risk of postoperative adverse events due to variability in lesion characteristics. Therefore, we conducted a retrospective study using propensity score matching evaluate efficacy clip preventing ESD. Methods This single-center cohort included 219 neoplasms which were removed by The patients allocated into and non-closure groups, compared before propensity-score...
In this study, we performed two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionisation time of fly mass spectrometry to identify the protein(s) associated with development oral squamous cell carcinomas (OSCCs) by comparing patterns OSCC-derived lines normal keratinocytes (NOKs), found that downregulation ubiquitous mitochondrial creatine kinase (CKMT1) could be a good candidate. Decreased levels CKMT1 mRNA protein were detected in all examined (n=9) when compared...
Runt-related transcription factor 2 (RUNX2) haploinsufficiency causes cleidocranial dysplasia (CCD) which is characterized by supernumerary teeth, short stature, clavicular dysplasia, and osteoporosis. At present, as a therapeutic strategy for osteoporosis, mesenchymal stem cell (MSC) transplantation therapy performed in addition to drug therapy. However, MSC-based osteoporosis CCD patients difficult due reduction the ability of MSCs differentiate into osteoblasts resulting from impaired...
We analyzed the mutational and methylation status of spleen tyrosine kinase (Syk) gene both mRNA protein levels in primary oral squamous cell carcinoma (OSCC) OSCC-derived lines examined function Syk vitro. Using quantitative real-time reverse transcription polymerase chain reaction, Western blotting immunofluorescence on 7 normal keratinocytes (NOKs), expression were commonly downregulated all compared to NOKs. Although no sequence variation coding region was identified these lines, we...
Abstract Using proteomic selection, functional verification, and clinical validation, we identified specific down-regulation of Lin-7C/VELI3/MALS-3 (Lin-7C), which marks oral squamous cell carcinoma (OSCC) metastasis. Despite a rarity sequence variations in the Lin-7C gene both primary OSCC OSCC-derived cells, high prevalence hypermethylation was detected CpG island region that strongly correlated with its down-regulation. Inducible mRNA by experimental demethylation found all cells tested....
In our previous rodent studies, the paclitaxel (PTX)-incorporating polymeric micellar nanoparticle formulation NK105 had showed significantly stronger antitumor effects and reduced peripheral neurotoxicity than PTX dissolved in Cremophor® EL ethanol (PTX/CRE). Thus, to elucidate mechanisms underlying due NK105, we performed pharmacokinetic analyses of PTX/CRE rats. Among neural tissues, highest concentrations were found dorsal root ganglion (DRG). Moreover, exposure DRG (Cmax_PTX...
Abstract Background Immune checkpoint inhibitors (ICIs) can induce immune-related adverse events (irAEs) including thyroid dysfunction. There are only a few reports on Graves’ disease induced by ICIs. We report case of new-onset after the initiation nivolumab therapy in patient receiving gastric cancer treatment. Case presentation The was 66-year-old Japanese man, who administered (240 mg every 3 weeks) as third-line for stage IVb cancer. His function normal before therapy. However, he...
Functional proteomics is a useful method to explore changes in protein expression human diseases, including carcinomas. To identify tumor-associated proteins as biomarkers or molecular targets of oral squamous cell carcinomas (OSCCs), we performed two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization-time flight mass spectrometry. Comparison the profiles OSCC lines normal keratinocytes identified six with markedly different levels. Of proteins,...
NK012 is a micelle-forming macromolecular prodrug of 7-ethyl-10-hydroxy camptothecin (SN-38), an active metabolite irinotecan. It accumulated and retained in tumor tissues gradually releases SN-38 enzyme-independent manner. was previously demonstrated to have stronger antitumor activity than irinotecan broad range human solid-tumor xenograft models. In our study, we used orthotopic multiple myeloma (MM) model created by injecting CD138-positive U266B1, cell line that produces IgE lambda...
Gorlin syndrome is an autosomal dominant inherited that predisposes a patient to the formation of basal cell carcinomas, odontogenic keratocysts, and skeletal anomalies. Causative mutations in several genes associated with sonic hedgehog (SHH) signaling pathway, including PTCH1, have been identified patients. However, no definitive genotype-phenotype correlations are evident these patients, their clinical presentation varies greatly, often leading delayed diagnosis treatment. We generated...
In the present study, in order to identify novel diagnostic biomarkers for malignant behavior of oral squamous cell carcinoma (OSCC), we determined proteomic profiles several OSCC lines and keratinocytes by two-dimensional fluorescence difference gel electrophoresis liquid chromatography tandem mass spectrometry. The protein expression level α-1-microglobulin/bikunin precursor (AMBP) was found be significantly lower than keratinocytes, a significant decrease AMBP mRNA confirmed RT-qPCR. To...
Hangeshashinto (TJ-14), a Kampo medicine comprising seven types of herbs, has been used in Japan to alleviate the side effects associated with anticancer drug treatments. However, pharmacological this currently remain unclear. The present study aimed demonstrate efficacy TJ-14 against drug-induced stomatitis, pain which may have negative impact on mastication and swallowing.Mucositis was induced Sprague-Dawley rats by cancer chemotherapy. Changes body weight, stomatitis grades,...
The purpose of this study was to investigate the molecular biological characteristics malignant mucosal melanoma (MMM) and adenoid cystic carcinoma (ACC) head neck. We analyzed common genetic abnormalities that may help identify loci in genes involved development MMM ACC neck by PCR-LOH on chromosomes 1p, 6q, 9p, 10q, 11q, 12q, 17p, 19q. LOH observed 6 12 cases 15 informative for at least one analyzed. One distinct deleted region identified chromosome 9p21. In addition, a possible...
Gastric endoscopic full-thickness resection (EFTR) requires secure defect closure [1]. Here, we report a successful case of EFTR with hand suturing that provided double-layer [2] [3] [4].
Abstract Aims During intraoperative bleeding in endoscopic submucosal dissection (ESD), switching to spray coagulation may be beneficial compared with the continuous use of swift and can reduce need for hemostatic forceps. We retrospectively assessed effectiveness modes on during gastric ESD. Methods Results A total 316 events (156 Swift group 160 Spray group) were consecutively recorded. In group, hemostasis was performed using mode a retracted tip needle‐type knife, followed by treated...