A5043476026- Neurotransmitter Receptor Influence on Behavior
- Advanced Fluorescence Microscopy Techniques
- Protease and Inhibitor Mechanisms
- Functional Brain Connectivity Studies
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Neuropeptides and Animal Physiology
- Digital Holography and Microscopy
- Genetics and Neurodevelopmental Disorders
- Signaling Pathways in Disease
- Receptor Mechanisms and Signaling
- Optical measurement and interference techniques
- Adipose Tissue and Metabolism
- Tissue Engineering and Regenerative Medicine
- Photoacoustic and Ultrasonic Imaging
- Traumatic Brain Injury and Neurovascular Disturbances
- Optical Polarization and Ellipsometry
- S100 Proteins and Annexins
- Neural dynamics and brain function
- Optical Coherence Tomography Applications
- Neurogenesis and neuroplasticity mechanisms
- Muscle Physiology and Disorders
- Colorectal Cancer Treatments and Studies
- Optical Imaging and Spectroscopy Techniques
- Ubiquitin and proteasome pathways
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
2014-2025
Polish Academy of Sciences
2013-2025
University of Calgary
2007
Abstract Whole-brain imaging with light-sheet fluorescence microscopy and optically cleared tissue is a new, rapidly developing research field. Whereas successful attempts to clear image mouse brain have been reported, similar result for rats has proven difficult achieve. Herein, we report on creating novel transgenic rat harboring fluorescent reporter GFP under control of neuronal gene promoter. We then present data clearing the brain, showing that FluoClearBABB was found superior over...
Abstract Over the recent years, fluorescence microscopy has seen substantial progress, transitioning from primarily qualitative analysis to a profoundly quantitative methodology, yet limitations in imaging depth and data quality persist due tissue opacity. Tissue optical clearing (TOC) methodologies were implemented overcome this hurdle, enabling of large samples such as entire murine organs. Further improvements, including expansion merge TOC benefits with improved resolution, converting...
Posttraumatic epilepsy (PTE) is a recurrent seizure disorder that often develops secondary to traumatic brain injury (TBI) caused by an external mechanical force. Recent evidence shows the extracellular matrix plays major role in remodeling of neuronal connections after injury. One proteases presumably responsible for this process metalloproteinase-9 (MMP-9). The levels MMP-9 are elevated rodent tissue and human blood samples TBI. However, no studies have described influence on development...
Many fundamental questions on alcohol use disorder (AUD) are frequently difficult to address by examining a single brain structure, but should be viewed from the whole perspective. c-Fos is marker of neuronal activation. Global profiling in rodents represents promising platform study functional networks rearrangements AUD. We used mouse model drinking IntelliCage. trained mice voluntarily drink alcohol, next subjected them withdrawal and reexposure. have developed dedicated image...
We have previously shown that Ttyh1 mRNA is expressed in neurons and its expression up-regulated the brain during epileptogenesis epilepsy. In this study, we aimed to elucidate role of neurons. found widespread protein vivo vitro. immunoreactivity vitro was frequently invaginations dendritic spines; however, Ttyh1, seldom co-localized with synaptic markers vivo. Silencing siRNA hippocampal cultures resulted alterations MAP2 distribution along neurites causing it appear form chains beads....
Traumatic brain injury (TBI) occurs when a blow to the head causes damage. Apart from physical trauma, it wide range of cognitive, behavioral, and emotional deficits including impairments in learning memory. On neuronal level, TBI may lead circuitry remodeling effect imbalance between excitatory inhibitory neurotransmissions. Such change homeostasis often disorders. The basic units connectivity are dendritic spines that tiny protrusions forming synapses two cells network. Spines dynamic...
Abstract An intra-hippocampus injection of kainic acid serves as a model status epilepticus and the subsequent development temporal lobe epilepsy. Matrix metalloproteinase-9 (MMP-9) is an enzyme that controls remodeling extracellular milieu under physiological pathological conditions. In response to brain insult, MMP-9 contributes synaptic plasticity may play role in progression epileptic condition. Marimastat metalloproteinase inhibitor was tested clinical trials cancer. The present study...
Abstract Alcohol dependence is characterized by the abnormal release of dopamine in brain reward‐related areas. Trace amine‐associated receptor 1 (TAAR1) a G protein‐coupled that negatively regulates neurotransmission and thus promising target treatment drug addiction. However, role TAAR1 regulation alcohol abuse remains understudied. Here, we assessed effect activation on drinking behaviours C57Bl/6J female mice housed IntelliCages. The animals were administered with either vehicle or full...
Whole‐organ and whole‐body optical tissue clearing methods allowing imaging in 3 dimensions are an area of profound research interest. Originally developed to study nervous tissue, they have been successfully applied all murine organs, yet rat peripheral organs is less advanced. Here, a modification CUBIC protocol presented. It provides rapid simple approach clear the entire adult organism thus within as little 4 days. Upgraded perfusion‐based preserves both anatomical structure signal from...
Laser-based lensless digital holographic microscopy (LDHM) is often spoiled by considerable coherent noise factor. We propose a novel LDHM method with significantly limited artifacts, e.g., speckle and parasitic interference fringes. It achieved incorporating rotating diffuser, which introduces partial spatial coherence preserves high temporal of laser light, crucial for credible in-line hologram reconstruction. present the first implementation classical diffuser concept in LDHM, increasing...
Activation of adaptive immunity is a complex process coordinated at multiple levels in both time and the three-dimensional context reactive lymph nodes (LNs). Although microscopy-based visualization its spatiotemporal dynamics unravels complexities developing immune response, such approach highly limited by light-obstructing nature tissue components. Recently, optical clearing (TOC) techniques were established to bypass this obstacle now allow image quantify entire murine organs with...
Understanding gene-environment interactions in the pathogenesis of schizophrenia remains a major research challenge. Matrix metalloproteinase-9 (MMP-9) has been previously implicated pathophysiology schizophrenia. In present study, adolescent Mmp-9 heterozygous mice, with genetically lower level MMP-9, were subjected to resident-intruder psychosocial stress for 3 weeks and then examined behavioral tests that evaluated cognitive deficits positive- negative-like symptoms Cognitive positive...
Optical diffraction tomography (ODT) enables non-invasive information-rich 3D refractive index (RI) reconstruction of unimpaired transparent biological and technical samples, crucial in biomedical research, optical metrology, materials sciences, other fields. ODT bypasses the inherent limitations 2D integrated quantitative phase imaging methods. To increase signal-to-noise ratio easy-to-implement common-path shearing interferometry setups are successfully combined with low spatiotemporal...
For many years optimal treatment for dysfunctional skeletal muscle characterized, example, by impaired or limited regeneration, has been searched. Among the crucial factors enabling its development is finding appropriate source of cells, which could participate in tissue reconstruction serve as an immunomodulating agent (limiting immune response well fibrosis, that is, connective formation), after transplantation to regenerating muscles. MSCs, including those derived from bone marrow, are...
Abstract Although mice are widely used to elucidate factors contributing penile disorders and develop treatment options, quantification of tissue changes upon intervention is either limited minuscule volume (histology) or acquired with spatial resolution (MRI/CT). Thus, imaging method suitable for expeditious acquisition the entire mouse penis subcellular described that relies on both aqueous‐ (clear, unobstructed brain cocktails computational analysis) solvent‐based (fluorescence‐preserving...
Abstract Many fundamental questions on addiction development are still unanswered. These frequently difficult to address by examining a single brain structure, but can best be addressed at the systems level. Neurons create functional networks that change over time, since regions may work together differently in different contexts. We offer framework for describing nature behind alcohol binge drinking and transition addiction. The present study investigated whole-brain c-Fos expression...