A5061605978- Zebrafish Biomedical Research Applications
- T-cell and B-cell Immunology
- Immune cells in cancer
- Pluripotent Stem Cells Research
- Receptor Mechanisms and Signaling
- Hematopoietic Stem Cell Transplantation
- Acute Myeloid Leukemia Research
- Single-cell and spatial transcriptomics
- Immune Cell Function and Interaction
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- CRISPR and Genetic Engineering
- Mast cells and histamine
- Pregnancy and preeclampsia studies
- Erythrocyte Function and Pathophysiology
- Mesenchymal stem cell research
- Hemoglobinopathies and Related Disorders
- Aquaculture disease management and microbiota
- Epigenetics and DNA Methylation
- Viral Infectious Diseases and Gene Expression in Insects
University of Edinburgh
2018-2023
MRC Centre for Regenerative Medicine
2023
Centre for Inflammation Research
2018-2022
Edinburgh College
2020
Queen's Medical Centre
2018
Whereas hundreds of cells in the mouse embryonic aorta transdifferentiate to hematopoietic cells, only very few establish stem cell (HSC) identity at a single time point. The Gata2 transcription factor is essential for HSC generation and function. In contrast surface-marker-based isolation, Gata2-based enrichment provides direct link internal regulatory network. Here, we use iterations index-sorting Gata2-expressing intra-aortic cluster (IAHC) single-cell transcriptomics, functional analyses...
Abstract Integrated molecular signals regulate cell fate decisions in the embryonic aortic endothelium to drive hematopoietic stem (HSC) generation during development. The G-protein–coupled receptor 56 (Gpr56, also called Adgrg1) is most highly upregulated gene cells that take on and expressed by adult bone marrow HSCs. Despite requirement for Gpr56 stem/progenitor (HS/PC) zebrafish embryos expression of GPR56 treatment-resistant leukemic patients, its function normal mammalian hematopoiesis...
Highlights•Efficient mast cell production is achieved with novel Gata2-reporter PSCs•14-day PSC culture produces cells and self-renewing progenitors•Gata2-reporter have high protease content degranulate•Gata2-reporter iPSC method advances prospects for human researchSummaryMast are tissue-resident immune cells. Their overgrowth/overactivation results in a range of common distressing, sometimes life-threatening disorders, including asthma, psoriasis, anaphylaxis, mastocytosis. Currently, drug...
ABSTRACT Hematopoietic stem cells (HSCs) develop from the hemogenic endothelium in cluster structures that protrude into embryonic aortic lumen. Although much is known about molecular characteristics of developing hematopoietic cells, we lack a complete understanding their origin and three-dimensional organization niche. Here use advanced live imaging techniques organotypic slice cultures, clonal analysis, mathematical modelling to show two-step process intra-aortic (IACH) formation. First,...
Abstract Integrated molecular signals regulate cell fate during embryonic hematopoietic stem (HSC) generation. The G-protein coupled receptor 56 (Gpr56) is the most highly-upregulated gene in cells that take on and it expressed by adult bone marrow HSCs. Although Gpr56 required for stem/progenitor (HS/PC) generation zebrafish embryos, its function mammalian hematopoiesis remains unclear. Here we examine role of HS/PC development conditional knockout (cKO) mouse embryos Gpr (KO) (ESC)...
Background: GPR56 (ADGRG1) is a widely expressed adhesion G-coupled protein receptor in humans and mice which implicated to play role both haematopoietic development Acute Myeloid Leukaemia (AML). In AML, there exists low frequency subpopulation of leukaemic stem cells (LSCs) which, similar their benign Hematopoietic Stem Cell (HSC) counterparts, exhibit cell behaviours. High expression forms part the molecular signature these LSCs, AML cases with high levels at diagnosis have poorer...