A5015329541- Biofuel production and bioconversion
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Clostridium difficile and Clostridium perfringens research
- Microbial Metabolic Engineering and Bioproduction
- Bacteriophages and microbial interactions
- Enzyme Production and Characterization
- RNA and protein synthesis mechanisms
- Genetics, Bioinformatics, and Biomedical Research
- Enzyme Catalysis and Immobilization
- Biochemical and Molecular Research
- Studies on Chitinases and Chitosanases
- Probiotics and Fermented Foods
- Philosophy and History of Science
- Fermentation and Sensory Analysis
- Plant nutrient uptake and metabolism
- 3D IC and TSV technologies
- Modular Robots and Swarm Intelligence
- Polysaccharides and Plant Cell Walls
- Adhesion, Friction, and Surface Interactions
- Science and Science Education
Genoscope
2013-2019
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2013-2019
Centre National de la Recherche Scientifique
2014-2019
CEA Paris-Saclay
2019
Université Paris-Saclay
2017-2019
Génomique Métabolique du Genoscope
2019
Université d'Évry Val-d'Essonne
2014-2017
Microbial metabolism of plant polysaccharides is an important part environmental carbon cycling, human nutrition, and industrial processes based on cellulosic bioconversion. Here we demonstrate a broadly applicable method to analyze how microbes catabolize that integrates carbohydrate-active enzyme (CAZyme) assays, RNA sequencing (RNA-seq), anaerobic growth screening. We apply this study the bacterium Clostridium phytofermentans ferments biomass components including glucans, mannans, xylans,...
Abstract Bacteria respond to their environment by regulating mRNA synthesis, often altering the genomic sites at which RNA polymerase initiates transcription. Here, we investigate genome-wide changes in transcription start site (TSS) usage Clostridium phytofermentans , a model bacterium for fermentation of lignocellulosic biomass. We quantify expression nearly 10,000 TSS single base resolution Capp-Switch sequencing, combines capture synthetically capped 5′ fragments with template-switching...
Increasing the resistance of plant-fermenting bacteria to lignocellulosic inhibitors is useful understand microbial adaptation and develop candidate strains for consolidated bioprocessing. Here, we study improve inhibitor in Clostridium phytofermentans (also called Lachnoclostridium phytofermentans), a model anaerobe that ferments biomass. We survey this bacterium panel biomass then evolve grow increasing concentrations lignin phenolic, ferulic acid, by automated, long-term growth selection...
Recycling of plant biomass by a community bacteria and fungi is fundamental to carbon flow in terrestrial ecosystems. Here we report how the fermenting, soil bacterium Clostridium phytofermentans enhances growth on cellulose simultaneously lysing consuming model from soil. We investigate mechanism fungal lysis show that among dozens different glycoside hydrolases C. secretes cellulose, most highly expressed enzymes degrade rather than substrates. These enzymes, GH18 Cphy1799 Cphy1800,...
ABSTRACT Novel processing strategies for hydrolysis and fermentation of lignocellulosic biomass in a single reactor offer large potential cost savings production biocommodities biofuels. One critical challenge is retaining high enzyme the presence elevated product titers. Toward this goal, cellulolytic, ethanol-producing bacterium Clostridium phytofermentans was adapted to increased ethanol concentrations. The resulting ethanol-tolerant (ET) strain has nearly doubled tolerance relative...
The mechanisms by which bacteria uptake solutes across the cell membrane broadly impact their cellular energetics. Here, we use functional genomic, genetic, and biophysical approaches to reveal how Clostridium (Lachnoclostridium) phytofermentans, a model bacterium that ferments lignocellulosic biomass, uptakes plant hexoses using highly specific, nonredundant ATP-binding cassette (ABC) transporters. We analyze transcription patterns of its 173 annotated sugar transporter genes find those...
Clostridia are a group of Gram-positive anaerobic bacteria medical and industrial importance for which limited genetic methods available. Here, we demonstrate an approach to make large genomic deletions insertions in the model Clostridium phytofermentans by combining designed II introns (targetrons) Cre recombinase. We apply these delete 50-gene prophage island programming targetrons position markerless lox66 lox71 sites, mediate deletion intervening 39-kb DNA region using Gene expression...