A5035755763- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Microbial Metabolic Engineering and Bioproduction
- Anaerobic Digestion and Biogas Production
- Polysaccharides and Plant Cell Walls
- Protein Hydrolysis and Bioactive Peptides
- Seed Germination and Physiology
- Rice Cultivation and Yield Improvement
- Microbial Metabolites in Food Biotechnology
- Enzyme-mediated dye degradation
- Phytase and its Applications
- Enzyme Catalysis and Immobilization
- Animal Nutrition and Physiology
- Plant responses to water stress
- Livestock and Poultry Management
- Drug Transport and Resistance Mechanisms
- Microbial Inactivation Methods
- Animal Genetics and Reproduction
- RNA and protein synthesis mechanisms
- Ga2O3 and related materials
- GABA and Rice Research
- Hybrid Renewable Energy Systems
- Microbial metabolism and enzyme function
- Microbial Community Ecology and Physiology
- Bacterial Genetics and Biotechnology
National Institute for Biotechnology and Genetic Engineering
2006-2024
Pakistan Institute of Engineering and Applied Sciences
2013-2021
University of Agriculture Faisalabad
1990-2006
Pir Mehr Ali Shah Arid Agriculture University
2006
The Ohio State University
1992
Nuclear Institute for Agriculture and Biology
1990-1992
Higher water requirements and increasing labor costs are the major problems of traditional rice production system. Direct seeded culture, growing without standing water, can be an attractive alternate. However, poor emergence seedling establishment, weed infestation main hindrances in adoption this culture. An attempt to improve performance direct by seed priming was made present study. Priming tools employed were soaking (soaking tap up radicle protrusion), hydropriming for 48 h,...
The influence of seed priming on germination, seedling vigor, ion homeostasis, and starch metabolism in coarse rice was studied. Priming treatments included pregermination (a traditional soaking method being used for nursery preparation), hydropriming 48 h, osmohardening with KCl or CaCl 2 (ψs –1.25 MPa solution) 24 ascorbate (10 mg·L –1 ) hardening h. Compared controls, all (except pregermination) reduced the time to start improved rate germination synchronization, length shoot root, fresh...
Fermenting agricultural biomass, such as rice straw (RS), to methane is a promising solution an ongoing waste problem. However, the biomass must first be pretreated break down lignin thereby increasing accessibility of substrate fermentative organisms. Biological pretreatment by microorganisms represents potentially economical strategy prepare for fermentation. We isolated seven candidate ligninolytic Bacillus sp. strains based on their robustness degradation. The production enzymes from...
HMt, a histone-related protein, has been isolated and characterized from Methanobacterium thermoautotrophicum delta H. HMt preparations contain two polypeptides designated HMt1 HMt2, encoded by the hmtA hmtB genes, respectively, that have cloned, sequenced, expressed in Escherichia coli. HMt2 are predicted to 68 67 amino acid residues, calculated molecular masses of 7,275 7,141 Da, respectively. Aligning sequences with HMf1 HMf2, subunit HMf, protein hyperthermophile Methanothermus fervidus,...
Impact of pre-sowing seed treatments on the germination and seedling vigour rice was evaluated through investigating biochemical changes following invigoration by analyzing sugars α-amylase activity. The seeds were invigorated traditional soaking, osmoconditioning (-1.1 MPa KNO3) for 24 or 48 h hardenening 18 h. All significantly affected rate spread, fresh dry weight roots shoots, shoot length, total, reducing nonreducing Seed hardening soaking performed best as indicated reduced time...
Anaerobic digestion is a process to convert organic biomass into bio-methane. Plenty of produced waste in Pakistan enough compensate energy thirst country and have potential replace costly fossil fuels. The lignocellulosic such as wheat straw, almond shell, sugarcane bagasse, maize straw corn cob were subjected bio-methane assay after proximate, ultimate chemical analysis. These fractions provide better understanding about theoretically predicating potentials neutral detergent fibre, acid...
Bio-methane from agricultural waste has enough potential to compete with other sources of energy.This study aims examine the bio-methane numerous wastes, including cotton waste, wheat bran, lentil straw, barley rice bran and peanut peels straw aim produce renewable energy solve disposal issues.The proximate, ultimate chemical composition analyses were performed predict theoretical biomethane potentials in silico.However, was experimentally assayed at mesophilic conditions.Moreover, elemental...
A putative protease gene ( aprE ) from the thermophilic bacterium Coprothermobacter proteolyticus was cloned and expressed in Bacillus subtilis . The enzyme determined to be a serine based on inhibition by PMSF. Biochemical characterization demonstrated that had optimal activity under alkaline conditions (pH 8–10). In addition, an elevated optimum temperature (60°C). also stable presence of many surfactants oxidant. Thus, C. has potential applications industries such as detergent market.
The thermodynamic and kinetic properties of solids state raw starch digesting alpha amylase from newly isolated Bacillus licheniformis RT7PE1 strain were studied. values<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:msub><mml:mi>Q</mml:mi><mml:mi>p</mml:mi></mml:msub></mml:mrow></mml:math>,<mml:math...
The present study was concerned with the searching of novel bacterial cultures from different samples for lab scale production pectinase. Keeping in view increasing demand pectinase specially Faisalabad, an industrial city Pakistan, isolation new hyper producer strains locally is easy and cheap way getting desirable products at low cost. Therefore, enzyme has been will be remained a part research every time. This method alone can also provide raw material further such as engineering or...
Abstract This study used conservative one variable-at-a-time and statistical surface response methods to increase the yields of an extracellular thermostable protease secreted by a newly identified thermophilic Bacillus subtilis BSP strain. Using conventional optimization techniques, physical parameters in submerged fermentation were adjusted at shake flask level reach 184 U/mL. These physicochemical further optimized methodology using Box Behnken design, yield increased 295 The was purified...
A bacterial endo-polygalacturonase (endo-PGase) gene from the plant pathogen Pectobacterium carotovorum was cloned into pGAPZαA vector and constitutively expressed in Pichia pastoris. The recombinant endo-PGase secreted by clone showed a 1.7 fold increase when culture medium included glycerol replacement of glucose as carbon source. enzyme had optimum activity at pH 5.5 40 °C with stability between 5.0 8.0 temperatures up to 50 °C. enhanced 41% addition 1 mM Co++, inhibited Fe++ 63%...