A5045545169- Field-Flow Fractionation Techniques
- Microfluidic and Bio-sensing Technologies
- thermodynamics and calorimetric analyses
- Microfluidic and Capillary Electrophoresis Applications
- Photodynamic Therapy Research Studies
- Electrical and Bioimpedance Tomography
- Nanoparticle-Based Drug Delivery
- Extracellular vesicles in disease
- Microbial Inactivation Methods
- MicroRNA in disease regulation
- Autophagy in Disease and Therapy
- Porphyrin and Phthalocyanine Chemistry
- Nanoplatforms for cancer theranostics
- Microwave and Dielectric Measurement Techniques
- Glioma Diagnosis and Treatment
- Cancer Cells and Metastasis
- 3D Printing in Biomedical Research
- Radiation Therapy and Dosimetry
- Chemical Reactions and Isotopes
- Nanopore and Nanochannel Transport Studies
- ATP Synthase and ATPases Research
- Mineral Processing and Grinding
- Antibiotics Pharmacokinetics and Efficacy
- Healthcare Systems and Practices
- Electrostatics and Colloid Interactions
Université de Limoges
2011-2024
Inserm
2022-2024
CNR de la Résistance aux Antibiotiques
2019-2022
Laboratoire COBRA
2022
Laboratoire de chimie des substances naturelles
2009-2017
Laboratoire de Chimie
2005-2009
Institut de Chimie des Substances Naturelles
2009
Glioblastoma is the most common primary brain tumor, characterized by its resistance to treatments. To define efficient therapy, origin of tumor-forming cells needs be elucidated in order search for new therapeutic pathways. The objective this study was determine different cell populations constituting a human glioblastoma line, U-87 MG and their sensitivity apoptosis induced through activation Fas, membranous death receptor. By sorting method, sedimentation field flow fractionation, two...
As is the case with most eucaryotic cells, cancer cells are able to secrete extracellular vesicles (EVs) as a communication means towards their environment and surrounding cells. EVs represented by microvesicles smaller called exosomes, which known for involvement in aggressiveness. The release of such requires intervention trafficking-associated proteins, mostly RAB-GTPases family. In particular, RAB27A its role addressing EVs-to-be secreted plasma membrane. this study, shRNAs targeting...
This paper introduces the first results of dielectric spectroscopy characterization glioblastoma cells, measuring their crossover frequencies in ultra-high-frequency range (above 50 MHz) by dielectrophoresis (DEP) techniques. Experiments were performed on two lines U87-MG and LN18 that cultured following different conditions, order to achieve phenotypic profiles. We demonstrate here presented DEP electrokinetic method can be used discriminate undifferentiated from differentiated cells. In...
Therapeutic resistance and infiltrative capacities justify the aggressiveness of glioblastoma. This is due to cellular heterogeneity, especially presence stemness-related cells, i.e. Cancer Stem Cells (CSC). Previous studies focused on autophagy its role in CSCs maintenance; these gave conflicting results as they reported either sustaining or disruptive effects. In present work, we silenced two related genes -either Beclin1 ATG5- by shRNA explored ensuing consequences markers' expression...
Cancer stem cells (CSCs) play critical roles in cancer, making them important targets for new diagnostic and therapeutic approaches. Since CSCs are heterogeneous not abundant tumors, few specific markers these currently exist, methods to isolate characterize required. To address this issue, we developed a label-free methodology isolate, enrich, identify from an tumor cell subpopulation using sorting method (sedimentation field flow fractionation, SdFFF) biosensor as detector. Enrichment was...
Neuroblastoma (NB) is the most common childhood solid tumor. Although spontaneous regression can occur in patients <1-year old, 70% of over age 1 year have a high-risk and difficult-to-treat NB. Cell type heterogeneity observed either morphological appearance NB tumors or cell lines isolated from tumor specimens. consists two principal neoplastic types: i) neuroblastic N-type (undifferentiated cells); ii) stromal S-type (differentiated cells). As cells seem to capacity differentiate...
This paper introduces the handling of a single cell by dielectrophoresis technics using UHF frequencies (above 100 MHz) inside microfluidic microsystems implemented on Bipolar-Complementary Oxide Semiconductor (BiCMOS) technology. Dielectrophoresis (DEP) is well-established technique used in kHz frequency range to manipulate, trap and sort electrically polarized cells exposing them non-uniform electric field. The novelty this work consists exploring capability signals discriminate based...
Cancer stem cells (CSCs) appear to be an essential target for cancer therapies, in particular, brain tumors such as Glioblastoma. Nevertheless, their isolation is made difficult by low content culture or (<5% of the tumor mass) and essentially based on use fluorescent magnetic labeling techniques, increasing risk differentiation induction. The label-free separation methods sedimentation field-flow fractionation (SdFFF) promising, but it becomes necessary consider a coupling with detection...
This paper reports on the application of dielectrophoresis techniques in radiofrequency range, order to probe inner dielectric specificities and therefore characterize individual biological cells. The novelty this work consists exploring capability UHF signals generate DEP-driven motion effects flowing cells a microfluidic micro-device. Additionally, with applied above 50MHz, distinct cross-over frequencies can be identified as function both cell type difference intracellular features,...
Cancer stem cells play a crucial role in tumor initiation, metastasis, and resistance to treatment. Cellular heterogeneity plasticity complicate the isolation of cancer cells. The impact intra-tumor cellular using label-free approach remains understudied context treatment resistance. Here, we use sedimentation field-flow fractionation technique separate, without labeling, cell subpopulations colorectal lines primary cultures according their biophysical properties. One three sorted exhibits...
Conventional antimicrobial susceptibility testing (AST) methods require 24-48 h to provide results, creating the need for a probabilistic antibiotic therapy that increases risk of resistance emergence. Consequently, development rapid AST has become priority. Over past decades, sedimentation field-flow fractionation (SdFFF) demonstrated high sensitivity in early monitoring induced biological events eukaryotic cell populations. This proof-of-concept study aimed at investigating SdFFF...
A novel microfluidic biosensing platform based on Bipolar-Complementary Oxide Semiconductor (BiCMOS) technology is presented. The device relies a quadruple electrode system and channel that are directly integrated into the back-end-of-line (BEOL) of BiCMOS stack. For proof concept repeatable electrical trapping single SW620 (colon cancer) cells in initially demonstrated. Additionally, for first time microwave intermodulation technique used high sensitivity dielectric spectroscopy, which...