Tomas Rypar

ORCID: 0000-0003-4817-2250
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About
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Research Areas
  • Biosensors and Analytical Detection
  • Advanced biosensing and bioanalysis techniques
  • Analytical chemistry methods development
  • Pesticide Residue Analysis and Safety
  • Plant Micronutrient Interactions and Effects
  • Aluminum toxicity and tolerance in plants and animals
  • Trace Elements in Health
  • Biopolymer Synthesis and Applications
  • Advanced Photocatalysis Techniques
  • Identification and Quantification in Food
  • Advanced Chemical Sensor Technologies
  • Tea Polyphenols and Effects
  • Microfluidic and Capillary Electrophoresis Applications
  • Quantum Dots Synthesis And Properties
  • Pharmaceutical Quality and Counterfeiting
  • Drug-Induced Adverse Reactions
  • Advanced Nanomaterials in Catalysis
  • Molecular Biology Techniques and Applications
  • Gold and Silver Nanoparticles Synthesis and Applications
  • Food Quality and Safety Studies
  • Analytical Methods in Pharmaceuticals
  • Forensic Fingerprint Detection Methods
  • Advanced Biosensing Techniques and Applications
  • SARS-CoV-2 detection and testing
  • MicroRNA in disease regulation

Mendel University in Brno
2021-2024

Central European Institute of Technology
2021-2022

Brno University of Technology
2021-2022

Short non-coding RNAs, specifically microRNAs (miRNAs), are of a great interest due to their presumed function in genome regulation. Moreover, miRNAs currently perceived as potential biomarkers for numerous diseases; variety detection methods and sensing systems have therefore been studied. We present magnetic-bead-based assay specific miRNA isolation coupled with sensitive electrophoretic analysis fluorescence detection. The magnetic separation step involves creating duplex targeted...

10.1166/jbn.2020.2872 article EN Journal of Biomedical Nanotechnology 2020-01-01

<title>Abstract</title> Balanced bacterial metabolism is essential for cell homeostasis and growth can be impacted by various stress factors. In this study, we investigated the impact of extended exposure <italic>Escherichia coli</italic> to zinc oxide (ZnO40) nanoparticles (ZnONPs40) on major metabolic pathways <italic>E. with aim simulate long-term conditions. Transcriptomic proteomic analyses along quantification intermediates tricarboxylic acid (TCA) were employed monitor study...

10.21203/rs.3.rs-4490206/v1 preprint EN Research Square (Research Square) 2024-07-16

microfluidic paper-based analytical devices modified with molecularly imprinted polymers (μPADs@MIPs) were developed for fluorescent detection of targeted thiols via in situ UV-induced formation quantum dots (μPADs@MIPs@QDs). The selectivity enhancement by the MIP layer formed on filter paper surface was demonstrated isolation L-homocysteine from wine. Followed addition metal precursors solution (Zn/Cd/Cu) and UV irradiation, thus enabling quantitative thiol (serving as a QD capping agent)....

10.2139/ssrn.4261998 article EN SSRN Electronic Journal 2022-01-01

Balancing the benefits of veterinary drugs, such as antibiotics, in food production with risks antibiotic resistance is an ongoing topic debate. Here, we utilized UV-induced fingerprint Spectroscopy (UV-IFS) to conduct a photochemical analysis 22 UV-IFS allows for monitoring changes spectral properties before and after exposure ultraviolet radiation (λ = 254 nm). This initiates reactions, resulting distinctive alterations enabling sample qualitative quantitative identification. Through this...

10.2139/ssrn.4673919 preprint EN 2023-01-01
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