A5085083042- Aquaculture disease management and microbiota
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Plant Virus Research Studies
- Plant Pathogenic Bacteria Studies
- Graphene and Nanomaterials Applications
- Plant Parasitism and Resistance
- Plant and animal studies
- Horticultural and Viticultural Research
- Ecology and Vegetation Dynamics Studies
- Fermentation and Sensory Analysis
- Plant-Microbe Interactions and Immunity
- Nanoparticles: synthesis and applications
- Viral gastroenteritis research and epidemiology
- Salmonella and Campylobacter epidemiology
- Identification and Quantification in Food
- Surgical Simulation and Training
- Dental Research and COVID-19
- Aquaculture Nutrition and Growth
- Plant Water Relations and Carbon Dynamics
- Polar Research and Ecology
- Genetics, Bioinformatics, and Biomedical Research
- Conducting polymers and applications
- Infection Control in Healthcare
- Healthcare and Environmental Waste Management
Pontificia Universidad Católica de Valparaíso
2015-2024
University of Chile
2010-2022
Hellenic Centre for Marine Research
2016-2020
Bacteriophages can influence the abundance, diversity and evolution of bacterial communities. Several bacteriophages have been reported to add virulence factors their host increase virulence. However, lytic also exert a selective pressure allowing proliferation strains with reduced This reduction be explained because use structures present on surface as receptors, which in different species. Therefore, modifications these receptors will resistant bacteriophage infection may exhibit...
Bacterial infections are a serious problem in aquaculture since they can result massive mortalities farmed fish and invertebrates. Vibriosis is one of the most common diseases marine hatcheries its causative agents bacteria genus Vibrio mostly entering larval rearing water through live feeds, such as Artemia rotifers. The pathogenic alginolyticus strain V1, isolated during vibriosis outbreak cultured seabream, Sparus aurata, was used host to isolate characterize two novel bacteriophages φSt2...
Pseudomonas syringae pv. actinidiae (Psa) is the causal agent of a bacterial canker in kiwifruit plants and has caused economic losses worldwide. Currently, primary strategies to control this pathogen include use copper-based compounds even antibiotics. However, emergence isolates Psa that are resistant these agrochemicals raised need for new alternatives pathogen. Bacteriophages have been proposed as an alternative infections agriculture, including Psa. Here, we show isolation...
Pseudomonas syringae is a Gram-negative bacterium that infects wide range of plants, causing significant economic losses in agricultural production. The pathogen exhibits high degree genetic and phenotypic diversity, which has led to the classification P. strains into different pathovars based on their host disease symptoms. Copper-based products have traditionally been used manage infections agriculture, but emergence copper-resistant become concern. Biological control promising strategy...
Vibrios are common inhabitants of marine and estuarine environments. Some them can be pathogenic to humans and/or animals using a broad repertory virulence factors. Lately, several reports have indicated that the incidence Vibrio infections in is rising also constitute continuing threat for aquaculture. Moreover, continuous use antibiotics has been accompanied by an emergence antibiotic resistance species, implying necessity efficient treatments. One promising alternative emerges lytic...
A clonal population of pathogenic Vibrio parahaemolyticus O3 : K6 serovar has spread in coastal waters, causing outbreaks worldwide since 1996. Bacteriophage infection is one the main factors affecting bacterial strain concentration ocean. We studied occurrence and properties phages infecting this V. pandemic waters. Analysing 143 samples, were found 13. All isolates clustered a closely related group podophages with at least 90% nucleotide sequence identity three essential genes, despite...
Vibrio parahaemolyticus is the leading cause of seafood-borne gastroenteritis worldwide. As reported in other countries, after rise and fall pandemic strain Chile, post-pandemic strains have been associated with clinical cases, including lacking major toxins TDH TRH. Since presence or absence tdh trh genes has used for diagnostic purposes as a proxy virulence V. isolates, understanding essential to detect these present water marine products avoid possible food-borne infection. In this study,...
Seafood consumption-related diarrhoea increased drastically in Chile when the pandemic strain of Vibrio parahaemolyticus serotype O3:K6 reached Region de Los Lagos, where most Chile's seafood is produced. Outbreaks peaked 2005 with 3725 clinical cases this region and gradually decreased to fewer than 10 2010 2011. We show here that concurrently vanished from mussels; we also report further environmental data. Integration 2010/2011 data those obtained since 2004 suggests after its arrival...
Bacteriophages have been proposed as an alternative to control pathogenic bacteria resistant antibiotics. However, they are not extensively used due different factors such vulnerability under environmental conditions and the lack of efficient administration methods. A potential solution is encapsulation bacteriophages in hydrogel polymers increase their viability a controlled release method. This work describes use alginate-Ca+ 2 matrixes mechanisms for protection dosification phage f3αSE...
The surveillance of Vibrio parahaemolyticus in the Chilean coast has been mainly performed by multiplex PCR amplification three different hemolysin genes, which are specie-specific virulence factors. These genes also employed determination V. pathogenic load seafood and for characterization strains associated to diarrhea cases human. During environmental that we every summer, occasionally observed a thermolabile (tlh) product slightly smaller size than expected, was coincident with low loads...
In recent years, Chilean kiwifruit production has been affected by the phytopathogen Pseudomonas syringae pv. actinidiae (Psa), which caused losses to industry. this study, we report genotypic and phenotypic characterization of 18 Psa isolates obtained from kiwifruits orchards between 2012 2016 different geographic origins. Genetic analysis multilocus sequence (MLSA) using four housekeeping genes (gyrB, rpoD, gltA, gapA) identification type III effector suggest that belong Biovar 3 cluster....
Vibrio parahaemolyticus non-toxigenic strains are responsible for about 10% of acute gastroenteritis associated with this species, suggesting they harbor unique virulence factors. Zonula occludens toxin (Zot), firstly described in cholerae, is a secreted that increases intestinal permeability. Recently, we identified Zot-encoding genes the genomes highly cytotoxic Chilean V. strains, including clinical strain PMC53.7. To gain insights into possible role Zot parahaemolyticus, analyzed whether...
Absorbed energy in excess of that used by photosynthesis induces photoinhibition, which is common water deficit conditions, resulting reductions stomatal conductance. In grapevines, controlled a field practice, but little known about the impact given shortage on transduction processes at leaf level relation to contrasting sensitivities drought. Here, we assessed effect nearly similar condition four grapevine varieties: Cabernet Sauvignon (CS) and Blanc (SB), are sensitive, Chardonnay (CH)...
Bacteriophages are an important factor in bacterial evolution. Some reports suggest that lytic bacteriophages can select for resistant mutant strains with reduced virulence. The present study explores the role of CHOED bacteriophage diversification and virulence its host
The focus of this meeting was to discuss the suitability using bacteriophages as alternative antimicrobials in agrifood sector. Following a One Health approach, workshop explored possibilities implementing phage application strategies agriculture, animal husbandry, aquaculture, and food production sectors. Therefore, had gathered researchers, representatives industry, policymakers debate advantages potential shortcomings alternatives traditional chemical pesticides. Industry delegates showed...
Salmonella spp. is a relevant foodborne pathogen with worldwide distribution. To mitigate infections, bacteriophages represent an alternative to antimicrobials and chemicals in food animals general. Bacteriophages (phages) are viruses that infect bacteria, which interact constantly their host. Importantly, the study of these interactions crucial for use phages as mitigation strategy. In this study, experimental coevolution Enteritidis ( S . Enteritidis) lytic phage was conducted tryptic soy...
Abstract Molybdenum disulfide (MoS 2 ) nanoparticles, obtained from liquid phase exfoliation in the presence of chitosan, were melt mixed with a linear low‐density polyethylene (LLDPE) matrix to produce novel antimicrobial active packaging materials. The LLDPE/MoS composites presented exfoliated nanoparticles forming aggregates that are well dispersed polymer matrix. These 2D‐layered MoS at concentrations 0.5, 1.0, and 3.0 wt% rendered several functionalities LLDPE, as for example an...