- Aquaculture disease management and microbiota
- Vibrio bacteria research studies
- Bacteriophages and microbial interactions
- Parasite Biology and Host Interactions
- Aquaculture Nutrition and Growth
- Myxozoan Parasites in Aquatic Species
- Identification and Quantification in Food
- Genomics and Phylogenetic Studies
- Reproductive biology and impacts on aquatic species
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial infections and disease research
- Environmental Toxicology and Ecotoxicology
- Cephalopods and Marine Biology
- Fish Biology and Ecology Studies
- Physiological and biochemical adaptations
- Aquatic life and conservation
- Parasites and Host Interactions
- Fish Ecology and Management Studies
- Antibiotics Pharmacokinetics and Efficacy
- Aquatic Invertebrate Ecology and Behavior
- Plant Virus Research Studies
- Marine Bivalve and Aquaculture Studies
- Leech Biology and Applications
- Turtle Biology and Conservation
- Microbial Community Ecology and Physiology
Hellenic Centre for Marine Research
2016-2025
Stavros Niarchos Foundation
2023
University of Crete
2020
University of Patras
2001-2004
University of Stirling
2004
Bacterial infections are a serious problem in aquaculture since they can result massive mortalities farmed fish and invertebrates. Vibriosis is one of the most common diseases marine hatcheries its causative agents bacteria genus Vibrio mostly entering larval rearing water through live feeds, such as Artemia rotifers. The pathogenic alginolyticus strain V1, isolated during vibriosis outbreak cultured seabream, Sparus aurata, was used host to isolate characterize two novel bacteriophages φSt2...
A monitoring program to follow vibriosis incidents in the Greek marine aquaculture was implemented over past 13 years. 273 isolates, from various cases originating eight regions and nine hosts, were collected characterized. The main species of survey European seabass (Dicentrarchus labrax) gilthead seabream (Sparus aurata). Various Vibrionaceae associated with vibriosis. Vibrio harveyi had highest prevalence isolated throughout year all hosts. During warm months, prevailed frequent...
Due to the emergence of multidrug-resistant bacteria, commonly known as "superbugs", phage therapy for control bacterial diseases rose in popularity. In this context, use phages management many important aquaculture environment is auspicious. Vibrio harveyi, a well-known and serious pathogen, responsible disease outbreaks aquaculture, resulting huge economic production losses. We isolated fully characterized novel bacteriophage, Virtus, infecting V. harveyi strain VH2. Virtus can infect wide...
Aquaculture is a burgeoning industry, requiring diversification into new farmed species, which are often at risk from infectious disease. We used mesocosm technique to investigate the susceptibility of sharpsnout seabream (Diplodus puntazzo) larvae potential environmental pathogens in seawater compared control borehole water. Fish exposed succumbed epitheliocystis 21 days post hatching, causing mortality quarter hosts. The pathogen responsible was not chlamydial, as found epitheliocystis,...
A mass mortality event (MME) affecting the fan mussel Pinna nobilis was first detected in Spain autumn 2016 and spread north- eastward through Mediterranean Sea. Various pathogens have been blamed for contributing to MME, with emphasis Haplosporidium pinnae , Mycobacterium sp. Vibrio spp. In this study, samples from 762 mussels (necropsies 263 individuals, mantle biopsies 499) of various health conditions, wide geographic age range, taken before during MME environments along Sea, were used...
The biology and biotechnology of bacteriophages have been extensively studied in recent years to explore new environmentally friendly methods controlling phytopathogenic bacteria. Pseudomonas syringae pv. tomato (Pst) is responsible for bacterial speck disease plants, leading decreased yield. Disease management strategies rely on the use copper-based pesticides. biological control Pst with could be an alternative approach diminish detrimental effects cultivations. lytic efficacy can used...
The haematological profile and a description of the cell types from peripheral blood six Mediterranean fish species are presented. highest haematocrit value was recorded in saupe, Sarpa salpa (P < 0.001), only herbivorous species, which also lacked monocyte cells. Eosinophils were absent European sea bass Dicentrarchus labrax. White bream, Diplodus sargus gilthead Sparus aurata had statistically significantly high numbers neutrophils low lymphocytes 0.001). different leucocyte not influenced...
Nineteen Vibrio anguillarum-specific temperate bacteriophages isolated across Europe and Chile from aquaculture environmental sites were genome sequenced analyzed for host range, morphology life cycle characteristics. The phages classified as Siphoviridae with sizes between 46,006 54,201 bp. All 19 showed high genetic similarity, 13 genetically identical. Apart sporadically distributed single nucleotide polymorphisms (SNPs), diversifications located in three variable regions (VR1, VR2 VR3)...
A novel virulent bacteriophage, vB_VspP_pVa5, infecting a strain of Vibrio splendidus was isolated from sea-cage aquaculture farm in Greece, and characterized using microbiological methods genomic analysis. Bacteriophage vB_VspP_pVa5 is N4-like podovirus with an icosahedral head measuring 85 nm length short non-contractile tail. The phage had narrow host range only the bacterial host, latent period 30 min burst size 24 virions per infected bacterium. Its genome 78,145 bp analysis identified...
Abstract The cortisol stress response and the molecular programming of corticoid axis were characterized for first time during early ontogeny in a Mediterranean marine teleost, European sea bass ( Dicentrarchus labrax ). Sea embryos, pre-larvae larvae at specific points development exposed to acute stressors temporal patterns whole body concentrations expression genes involved corticosteroid biosynthesis, degradation signaling determined. Expression gr1 , gr2 mr crf ) into regulation...
Abstract Following the intensification of Mediterranean aquaculture over years, Sparicotyle (syn. Microcotyle ) chrysophrii (van Beneden and Hesse, 1863; Microcotylidae, Monogenea) became one most pathogenic ectoparasites for gilthead seabream ( Sparus aurata L.) aquaculture. It has demonstrated a remarkable versatility to expand its known host range, accomplish an efficient transfer between wild farmed sparid populations wide geographic areas, establish itself as detrimental pathogen. Even...
In the present study, we examined a disease caused by Aeromonas veronii bv. sobria in sea cage-farmed European seabass Dicentrarchus labrax, Aegean Sea, Greece. Commercial sized fish were affected A. and exhibited high morbidity mortality. Gross pathologic features histology revealed systemic infection characterized presence of abscesses chronic granulomatous inflammation. Two clinical bacterial isolates (Aero NS Aero PDB) identified as based on bacteriological characteristics sequence...
The administration of antibiotics in aquaculture has raised concern about the impact their overuse marine ecosystems, seafood safety and consumers’ health. This “green consumerism” forced researchers to find new alternatives against fish pathogens. present study focused on 12 Mediterranean medicinal-aromatic plants as potential antimicrobials antioxidant agents that could be used aquaculture. In vitro assays showed essential oils (EOs) from all studied had anti-bacterial properties, with...
Vibrio harveyi is a Gram-negative marine bacterium that causes major disease outbreaks and economic losses in aquaculture. Phage therapy has been considered as potential alternative to antibiotics however, candidate bacteriophages require comprehensive characterization for safe practical phage therapy. In this work, lytic novel jumbo bacteriophage, vB_VhaM_pir03 belonging the Myoviridae family was isolated characterized against V. type strain DSM19623. It had broad host activity 31...