A5082179844- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Glycosylation and Glycoproteins Research
- Bacteriophages and microbial interactions
- Advanced Biosensing Techniques and Applications
- Animal Genetics and Reproduction
- Chemical Synthesis and Analysis
- Cell Adhesion Molecules Research
- Glioma Diagnosis and Treatment
- Traditional Chinese Medicine Analysis
- Chronic Kidney Disease and Diabetes
- Brain Metastases and Treatment
- TGF-β signaling in diseases
- Protein purification and stability
- Muscle metabolism and nutrition
- HER2/EGFR in Cancer Research
- CAR-T cell therapy research
- Diatoms and Algae Research
- Autophagy in Disease and Therapy
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- bioluminescence and chemiluminescence research
- Muscle Physiology and Disorders
- Peptidase Inhibition and Analysis
MacroGenics (United States)
2017-2025
Shanghai Jiao Tong University
2024
University of Illinois Chicago
2011-2016
Guangxi University
2015
Ashland (United States)
2015
Guangxi University of Chinese Medicine
2015
Our evidence demonstrated that CKD upregulated the expression of myostatin, TNF- α , and p-IkBa downregulated phosphorylation PI3K, Akt, FoxO3a, which were also associated with protein degradation muscle atrophy. The autophagosome formation autophagy-related genes increased in rats. mRNA level MAFbx MuRF-1 rats, as well proteasome activity 26S. Moreover, activation myostatin elicited by induces C2C12 myotube atrophy via upregulating genes, including MuRF1 subunits. Inactivation FoxO3a...
Mounting evidence supports the role of neuroinflammation in radiation-induced brain injury (RIBI), a chronic disease characterized by delayed and progressive neurological impairment. Asparagine endopeptidase (AEP), also known as legumain (LGMN), participates multiple malignancies neurodegenerative diseases may potentially be involved RIBI. Here, we found AEP expression was substantially elevated cortex hippocampus wild-type (
Background: Bispecific antibodies represent a promising class of biologics for cancer treatment. However, their dual specificity and complex structure pose challenges in the engineering process, often resulting molecules with good functional but poor physicochemical properties. Method: To overcome limitations properties an anti-5T4 x anti-CD3 (α5T4 αCD3) DART molecule, phage-display method was developed, which succeeded simultaneously cross-reactivity to cynomolgus 5T4 ortholog, improving...
Often when generating recombinant affinity reagents to a target, one singles out an individual binder, constructs secondary library of variants, and selects tighter or more specific binder. To enhance the throughput this general approach, we have developed integrated strategy where "affinity maturation" step is part phage-display pipeline, rather than follow-on process. In our new schema, perform two rounds selection, followed by error-prone PCR on pools recovered clones, generation...
The 'sandwich' binding format, which uses two reagents that can bind simultaneously to a given analyte, is the gold standard in diagnostics and many biochemical techniques. One of bottlenecks creating sandwich assay identifying pairs non-competitively target. To bridge this gap, we invented Megaprimer Shuffling for Tandem Affinity Reagents (MegaSTAR) identify non-competitive recombinant affinity through phage-display. key innovation MegaSTAR construction tandem library, are...
Affinity reagents of high affinity and specificity are very useful for studying the subcellular locations quantities individual proteins. To generate high-quality human Lyn tyrosine kinase, a phage display library fibronectin type III (FN3) monobodies was selected with recombinant form SH3 domain. While highly specific monobody, TA8, initially isolated, we chose to improve its through directed evolution. A secondary 1.2 × 109 variants constructed screened by selection, yielding three...