The extracellular matrix (ECM) forms through hierarchical assembly of small and larger polymeric molecules into a transient, hydrogel-like fibrous network that provides mechanical support biochemical cues to cells. Synthetic, supramolecular networks formed via non-covalent various are therefore potential candidates as synthetic mimics the natural ECM, provided functionalization with is effective. Here, combinations slow fast exchanging self-assemble fibers employed form transient hydrogel...

Small bioactive peptide sequences derived from extracellular matrix proteins possess the ability to interact with cell receptors. As such, these additives are excellent mimics develop materials for 3D culture. Two types of supramolecular modified collagen type I mimicking presented; UPy-GFOGER (39 amino acids), a novel superstructure, and more simplistic UPy-DGEA (7 acids). Here, we studied impact conformational differences between both additives, on their biological performance. Various...

Abstract Biomaterials that are commonly used for the attenuation of adverse remodeling or as regenerative treatment myocardial infarction (MI), often have capacity to release drugs in a sustained manner, providing strength and stability infarcted area, mimic extracellular matrix. Retention redistribution injected biomaterials is factor overlooked, but plays significant role effectiveness treatment. Wash‐out therapeutics from cardiac area can lead unwanted side‐effects therefore add insult...

Abstract Soluble biochemical agents are being employed to generate kidney organoids from human‐induced pluripotent stem cells. However, this soluble factor approach does not consider the effect of mechanical environment on lineage commitment. Here, a mechanoresponsive nano‐environment with cell‐adhesive properties composed supramolecular hydrogelators that co‐assemble into fibrous superstructures form transient network is presented, which used encapsulate organoids. The delayed sol‐gel...

The biochemical complexity of a material determines the biological response cells triggered by cell-material interaction.

Abstract Recent advances in the field of cardiac regeneration show great potential use injectable hydrogels to reduce immediate flush‐out injected factors, thereby increasing effectiveness encapsulated drugs. To establish a relation between function and retention drug‐encapsulating hydrogel, quantitative vivo imaging method is required. Here, supramolecular ureido‐pyrimidinone modified poly(ethylene glycol) (UPy‐PEG) material developed into bioactive hydrogel for radioactive large animal...

Viscoadaptation is an essential process in natural cells, where supramolecular interactions between cytosolic components drive adaptation of the cellular mechanical features to regulate metabolic function. This important relationship properties and function has until now been underexplored artificial cell research. Here, we have created platform that exploits internal display viscoadaptive behavior. As material mimic component these employed a pH-switchable hydrogelator based on...

Two synthetic supramolecular hydrogels, formed from bis-urea amphiphiles containing lactobionic acid (LBA) and maltobionic (MBA) bioactive ligands, are applied as cell culture matrices in vitro. Their fibrillary dynamic nature mimics essential features of the extracellular matrix (ECM). The carbohydrate self-assemble into long fibers water, hydrogels by physical entanglement through bundling. Gels both exhibit good self-healing behavior, but remarkably different stiffnesses. They display...

Structurally and functionally well-defined recombinant proteins are an interesting class of sequence-controlled macromolecules to which different crosslinking chemistries can be applied tune their biological properties. Herein, we take advantage a 571-residue peptide based on human collagen type I (RCPhC1), functionalized with supramolecular 4-fold hydrogen bonding ureido-pyrimidinone (UPy) moieties. By grafting UPy moieties onto the backbone RCPhC1 (UPy-RCPhC1), increased control over...

The cellular microenvironment is composed of a dynamic hierarchical fibrillar architecture providing variety physical and bioactive signals to the surrounding cells. This dynamicity, although common in biology, challenge control synthetic matrices. Here, responsive supramolecular monomers were designed that are able assemble into fibrous structures, combining fiber formation via hydrogen bonding interactions, with temperature-responsive hydrophobic collapse, resulting cross-linking hydrogel...

Expanding the bioactivation toolbox of supramolecular materials is utmost relevance for their broad applicability in regenerative medicines. This study explores functionality a peptide mimic Notch ligand Jagged1 system that based on hydrogen bonding ureido-pyrimidinone (UPy) units. The studied when formulated as an additive solid material and self-assembled solution. UPy conjugation DSLJAG1 sequence allows functionalization UPy-modified polycaprolactone, elastomeric material, with...

Abstract We report on the self‐assembly in water of a set bis‐urea amphiphiles. A range techniques, including dynamic light scattering, Cryo‐TEM, SAXS, and MS are used to study effect structural variation morphology assemblies. The length, dispersity, end‐group ethylene glycol hydrophilic part molecule, as well alkyl chain length varied tailor towards soluble wormlike micelles. Slight modification molecular structures gave large difference behavior water, giving guidelines for design rodlike...

The Spontaneous Symmetry Breaking (SSB) phenomenon is a natural event in which system changes its symmetric state, apparently reasonless, an asymmetrical one. Nevertheless, this occurrence could be hiding unknown inductive forces. An intriguing investigation pathway uses supramolecular aggregates of suitable achiral porphyrins, useful to mimic the light-harvesting systems (as chlorophyll). Using as SSB probe 5,10,15,20-tetrakis[p(ω-methoxypolyethyleneoxy)phenyl]porphyrin (StarP), non-ionic...

The design of photo-responsive supramolecular hydrogels based on coumarin dimerization and de-dimerization is described. unit chemically incorporated into an oligo(ethylene glycol) (OEG) bis-urea amphiphile that capable co-assembling with non-functionalized OEG amphiphile, to form fibers. UV light two different wavelengths (365 nm 254 nm) employed induce a photo-reversible process units, respectively. co-assembled solutions could be photo-crosslinked sol-to-gel transition through 365 light,...

Dynamicity plays a central role in biological systems such as the cellular microenvironment. Here, affinity and dynamics of different guest molecules transient supramolecular polymer hydrogel system, i.e. host network, are investigated. The system consists bifunctional ureido-pyrimidinone (UPy) poly(ethylene glycol) polymers. A monofunctional complementary UPy is introduced, designed to interact with network based on UPy-UPy interactions. Furthermore, two other synthesized, being cholesterol...

Abstract MicroRNAs (miRs) are potent regulators of biology and disease. The miR‐15 family is shown to regulate cardiomyocyte proliferation antimiR‐based inhibition induces a cardioprotective effect after myocardial infarction in mice. However, systemic delivery antimiRs leads accumulation kidneys liver, with relatively poor cardiac exposure. Injectable hydrogels proposed serve as sustained‐release drug depots can potentially be used improve efficacy antimiR therapeutics. Here, the...

Journal Article VitroJet: Moving Sample Preparation into the New Era Get access René Henderikx, Henderikx Cryosol-World, Weert, Netherlands Search for other works by this author on: Oxford Academic Google Scholar Maaike J G Schotman, Schotman Frank T Nijpels, Nijpels Bart W A M Beulen Corresponding author: bart.beulen@cryosol-world.com Microscopy and Microanalysis, Volume 29, Issue Supplement_1, 1 August 2023, Pages 1044–1045, https://doi.org/10.1093/micmic/ozad067.534 Published: 22 July 2023

Abstract Embedding biomolecules in vitreous ice of optimal thickness is critical for structure determination by cryo-electron microscopy. Ice assessment and selection suitable holes data collection are currently part time-consuming preparatory routines performed on expensive electron microscopes. To address this challenge, a routine has been developed to measure during sample preparation using an optical camera integrated the VitroJet. This method allows estimate with error below ± 20 nm...

Journal Article Controlled Cryo-EM Sample Preparation Using the VitroJet Get access Maaike J G Schotman, Schotman Cryosol-World B.V., Weert, Netherlands Search for other works by this author on: Oxford Academic Google Scholar Giulia Weissenberger, Weissenberger René M Henderikx, Henderikx Frank T Nijpels, Nijpels Bart W A Beulen Corresponding author: bart.beulen@cryosol-world.com Microscopy and Microanalysis, Volume 28, Issue S1, 1 August 2022, Pages 1200–1201,...