A5103171114- Hemoglobin structure and function
- Hemoglobinopathies and Related Disorders
- Erythrocyte Function and Pathophysiology
- Heme Oxygenase-1 and Carbon Monoxide
- Neonatal Health and Biochemistry
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- High Altitude and Hypoxia
- Neuroscience and Neuropharmacology Research
- Iron Metabolism and Disorders
- Endodontics and Root Canal Treatments
- Dental materials and restorations
- Traumatic Brain Injury and Neurovascular Disturbances
- Blood properties and coagulation
- Cardiac Arrest and Resuscitation
- Neuroscience of respiration and sleep
- Thermal Regulation in Medicine
- Vasculitis and related conditions
- Advanced Radiotherapy Techniques
- Medical Imaging Techniques and Applications
- Dental Implant Techniques and Outcomes
- Nanoplatforms for cancer theranostics
- Thyroid and Parathyroid Surgery
- Advanced X-ray and CT Imaging
- Optical Imaging and Spectroscopy Techniques
- Radiation Dose and Imaging
University of California, San Diego
2020-2024
University of Concepción
2022-2024
La Jolla Bioengineering Institute
2020-2024
Boston Children's Hospital
2022-2024
University of California San Diego Medical Center
2022
The Ohio State University
2021
Hospital Universitario de San Vicente Fundación
2020
Universidad de Antioquia
2020
Austral University of Chile
2011-2018
University of Tübingen
2013
In patients with the acute respiratory distress syndrome, massive alveolar collapse and cyclic lung reopening overdistention during mechanical ventilation may perpetuate injury. We determined whether a ventilatory strategy designed to minimize such injuries could reduce not only pulmonary complications but also mortality at 28 days in syndrome.
Objective To determine the effectiveness of chlorhexidine 0.12% mouthwash (CHX) after tooth extraction for prevention alveolar osteitis (AO). Material and methods We conducted a double-blind randomised clinical trial stratified by risk factors. enrolled cohort 822 patients who underwent dental extractions, were considered to be at developing AO (previous surgical site infection, traumatic extraction, tobacco smoking). After randomly allocated CHX group or placebo group, matched The primary...
Clinical Relevance The use of reduced viscosity composites, such as flowable and pre-heated resins, the first increment in Class II preparations, did not improve gingival margin adaptation nor it minimize gap formation at dentin-composite interface.
Alpha-alpha diaspirin-crosslinked human hemoglobin (DCLHb or ααHb) was a promising early generation red blood cell (RBC) substitute. The DCLHb developed through collaborative effort between the United States Army and Baxter Healthcare. core design feature underlying its development chemical stabilization of tetrameric structure (Hb) to prevent Hb intravascular dimerization extravasation. resuscitate warfighters on battlefield, who suffered from life-threatening loss. However, extensive...
ABSTRACT Background: Hemoglobin (Hb)-based oxygen carriers (HBOCs) have been proposed as alternatives to blood for decades. Previous studies demonstrated that large molecular diameter HBOCs based on polymerized bovine Hb (PolybHb) attenuate side-effects and toxicity. The objective of this study was test the safety efficacy tense state PolybHb after long-term storage. Methods Results: subjected diafiltration remove low weight (< 500 kDa) species stored 2 years. studied in parallel with...
Haptoglobin (Hp) is the plasma protein that binds and clears cell-free hemoglobin (Hb), whereas apohemoglobin (apoHb, i.e., Hb devoid of heme) can bind heme. Therefore, apoHb-Hp complex should facilitate holoHb-apoHb αβ-dimer exchange apoHb-heme intercalation. Thus, we hypothesized could both heme clearance, which, if not alleviated, have severe microcirculatory consequences. In this study, characterized Hb/heme ligand interactions assessed their in vivo binding with was studied transfer...
Introduction: The demand for red blood cells (RBCs) is on the rise due to increasing diagnosis of chronic diseases such as sickle cell anemia, malaria, and thalassemia. Despite many commercial attempts, there are no U.S. FDA-approved artificial RBCs use in humans. Existing RBC substitutes have employed various strategies transport oxygen, extend circulation time, reduce organ toxicity, but none replicated natural protective mechanisms RBCs, which prevent hemoglobin (Hb) dimerization heme...
// Alfredo Lucas 1 , Donald A. Belcher 2 Carlos Munoz Alexander T. Williams Andre F. Palmer and Pedro Cabrales Department of Bioengineering, University California San Diego, La Jolla, CA 92093, USA William G. Lowrie Chemical Biomolecular Engineering, The Ohio State University, Columbus, OH 43210, Correspondence to: Cabrales, email: pcabrales@ucsd.edu Keywords: chemotherapy; cisplatin; polymerized hemoglobin; non-small cell lung cancer; hypoxia Received: February 26, 2020 Accepted: June 15,...
Abstract Background Hemoglobin (Hb)‐based oxygen (O 2 ) carriers (HBOCs) are being developed as alternatives to red blood cells and when these products unavailable. Clinical trials of previous HBOC generations revealed side effects, including hypertension vasoconstriction, that were not observed in preclinical studies. Large molecular weight (MW) polymerized bovine Hb (PolybHb) represents a new class with promising results. We evaluated the safety profile PolybHb after an exchange...
The mechanical properties and deformability of Red Blood Cells (RBCs) are important determinants blood rheology microvascular hemodynamics. objective this study is to quantify the wall shear stress experienced by RBC membrane during capillary plug flow in vivo utilizing high speed video recording from intravital microscopy, biomechanical modeling, computational methods. Capillaries were imaged rat cremaster muscle pre- post-RBC transfusion stored RBCs for 2-weeks. contours extracted image...
Oxygen therapeutics are being developed for a variety of applications in transfusion medicine. In order to reduce the side effects (vasoconstriction, systemic hypertension, and oxidative tissue injury) associated with previous generations oxygen therapeutics, new strategies focused on increasing molecular diameter hemoglobin (Hb) obtained from mammalian sources via polymerization encapsulation. Another approach toward therapeutic design has centered using naturally occurring large Hbs [i.e.,...
Polymerized hemoglobin (PolyHb) is a promising (Hb)-based oxygen carrier currently undergoing development as red blood cell substitute. Unfortunately, commercially developed products are composed of low-molecular-weight (LMW) PolyHb molecules, which extravasate, scavenge nitric oxide, and result in vasoconstriction hypertension. The naturally occurring Hb-scavenging species haptoglobin (Hp), combined with the purified heme-scavenging apohemoglobin (apoHb), potential candidate to alleviate...
Abstract Background Hemolysis releases toxic cell‐free hemoglobin (Hb), heme, and iron, which overwhelm their natural scavenging mechanisms during acute or chronic hemolytic conditions. This study describes a novel strategy to purify protein cocktail containing comprehensive set of scavenger proteins for potential treatment hemolysis byproducts. Study Design Methods Tangential flow filtration was used from Human Cohn Fraction IV (FIV). A series in vitro assays were performed characterize...