- Environmental DNA in Biodiversity Studies
- Diatoms and Algae Research
- Microbial Community Ecology and Physiology
- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Genomics and Phylogenetic Studies
- Escherichia coli research studies
- Vibrio bacteria research studies
- Bacteriophages and microbial interactions
- Marine Ecology and Invasive Species
- Phytochemistry and Bioactivity Studies
- Protist diversity and phylogeny
- Biotin and Related Studies
- Wastewater Treatment and Nitrogen Removal
- Constructed Wetlands for Wastewater Treatment
- Identification and Quantification in Food
- Environmental Toxicology and Ecotoxicology
- Microplastics and Plastic Pollution
- Recycling and Waste Management Techniques
- Marine Biology and Environmental Chemistry
Cyprus University of Technology
2017-2025
Royal Botanic Garden Edinburgh
2021
Universitat de València
2021
Institute of Agrifood Research and Technology
2021
Brides-les-Bains
2021
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2021
Université de technologie de belfort-montbéliard
2021
Museo delle Scienze
2021
Imperial College London
2018
Medical Research Council
2018
DNA metabarcoding of benthic diatoms has been successfully applied for biomonitoring at the national scale and can now be considered technically ready routine application. However, protocols methods still vary between within countries, limiting their transferability comparability results. In order to overcome this, use diatom requires knowledge sources variability introduced by different steps procedure. Here, we examine how elements procedures contribute European laboratories. A set four...
Significance The type VI secretion system (T6SS) is a molecular weapon used for interbacterial competition. It acts like crossbow bolt to deliver toxic effectors into target cells. Here we characterize T6SS toxin which call Tse7. We show that it DNase and its two-domain PAAR-nuclease structure allows coupling the VgrG tip via PAAR domain. Disruption of this interface abrogates delivery results in loss killing ability. identify corresponding immunity protein, Tsi7, directly interacts with...
The bacterial type VI secretion system (T6SS) delivers effectors into eukaryotic host cells or toxins competitor for survival and fitness. T6SS is positively regulated by the threonine phosphorylation pathway (TPP) negatively T6SS-accessory protein TagF. Here, we studied mechanisms underlying TagF-mediated repression in two distinct pathogens, Agrobacterium tumefaciens Pseudomonas aeruginosa. We found that A. tumefaciens, toxin T6SS-dependent antibacterial activity are suppressed a...
Biofouling poses a significant economic threat to various marine industries, leading financial losses that can reach billions of euros annually. This study highlights the urgent need for effective alternatives traditional antifouling agents, particularly following global ban on organotin compounds.
Type III secretion systems (T3SSs) are essential devices in the virulence of many Gram-negative bacterial pathogens. They mediate injection protein effectors from bacteria into eukaryotic host cells to manipulate them during infection. T3SSs involved (vT3SSs) evolutionarily related flagellar export apparatuses (fT3SSs), which for assembly and cell motility. The structure external transmembrane parts both fT3SS vT3SS is increasingly well-defined. However, arrangement their cytoplasmic inner...
Current knowledge on the capacity of plastics as vectors microorganisms and their ability to transfer between different habitats (i.e. air, soil river) is limited. The objective this study was characterise evolution bacterial community adhered environmental [low-density polyethylene (LDPE)] across environments from point use receiving environment destination in sea. took place a typical Mediterranean intermittent river basin Larnaka, Cyprus, characterised by large greenhouse area whose...
During the past decade genetic approaches have been developed to monitor biodiversity in aquatic ecosystems. These enable access taxonomic and information from biological communities using DNA environmental samples (e.g. water, biofilm, soil) methods based on high-throughput sequencing technologies, such as metabarcoding. Within context of Water Framework Directive (WFD), could be applied assess Biological Quality Elements (BQE). are used indicators ecological status ecosystems part national...
Environmental conditions, such as nutrient concentrations, salinity, elevation etc., shape diatom assemblages of periphytic biofilms. These respond rapidly to environmental changes, a fact which makes diatoms valuable bioindicators. Hence, freshwater biomonitoring programmes currently use indices (e.g. EU Water Framework Directive - WFD). To date, microscopy-based assessments require high taxonomic expertise for identification at the species level. High-throughput technologies now provide...
In freshwater ecosystems, periphytic biofilms include diatom assemblages that depend on environmental conditions (e.g., nutrient concentrations, salinity, temperature etc.). These respond rapidly to changes, which makes diatoms valuable bioindicators. For this reason, they are currently used in biomonitoring programs EU Water Framework Directive - WFD) (Foster et al., 2000). To date, taxonomic identification is based morphological criteria, requires high expertise identify them the species...
Environmental conditions, such as nutrient concentrations, salinity, elevation etc., shape diatom assemblages of periphytic biofilms.These respond rapidly to environmental changes, a fact which makes diatoms valuable bioindicators.Hence, freshwater biomonitoring programmes currently use indices (e.g.EU Water Framework Directive -WFD).To date, microscopy-based assessments require high taxonomic expertise for identification at the species level.High-throughput technologies now provide...
Diatoms are unicellular eukaryotic organisms, which have been exploited over the years for effective freshwater bioassessment. Therefore, they excellent bioindicators, routinely used in national environmental monitoring programs all Europe within Water Framework Directive (WFD) 2000/60 /EC (Foster et al., 2000) and CEN standards (CEN, 2018). Over years, new technologies introduced to this field maximise improve time cost required The application of DNA metabarcoding characterisation benthic...