A5004272755- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Microplastics and Plastic Pollution
- Bacterial Genetics and Biotechnology
- Yersinia bacterium, plague, ectoparasites research
- biodegradable polymer synthesis and properties
- Mycobacterium research and diagnosis
- Fungal Infections and Studies
- Antifungal resistance and susceptibility
- Graphene and Nanomaterials Applications
- RNA and protein synthesis mechanisms
- Sports Performance and Training
- Global Public Health Policies and Epidemiology
- Biotin and Related Studies
- Antimicrobial Resistance in Staphylococcus
- Escherichia coli research studies
- Genomics and Phylogenetic Studies
- Exercise and Physiological Responses
- Recycling and Waste Management Techniques
- Sports injuries and prevention
- Antimicrobial agents and applications
Brunel University of London
2023-2025
University of London
2025
Centre for Inflammation Research
2023
Imperial College London
2017-2021
University of Manchester
2006
Significance Bacteria evolved molecular weapons to help them thrive in polymicrobial environments. The type VI secretion system (T6SS) is a gun loaded with great diversity of bacterial toxins. On contact neighboring cells, toxins are fired, and the absence immunity, prey killed, allowing attacker prevail. Each bacterium can be equipped several distinct T6SSs, it unclear whether they simultaneously active or each has specific role particular environment. Here we showed that production three...
Significance The type VI secretion system (T6SS) is a molecular weapon used for interbacterial competition. It acts like crossbow bolt to deliver toxic effectors into target cells. Here we characterize T6SS toxin which call Tse7. We show that it DNase and its two-domain PAAR-nuclease structure allows coupling the VgrG tip via PAAR domain. Disruption of this interface abrogates delivery results in loss killing ability. identify corresponding immunity protein, Tsi7, directly interacts with...
The type VI secretion system (T6SS) is crucial in interbacterial competition and a virulence determinant of many Gram-negative bacteria. Several T6SS effectors are covalently fused to secreted structural components such as the VgrG spike for delivery into target cells. In Pseudomonas aeruginosa, VgrG2b effector was previously proposed mediate bacterial internalization eukaryotic this work, we find that C-terminal domain (VgrG2bC-ter) elicits toxicity periplasm, counteracted by cognate...
Abstract Plastic pollution is an increasing global issue desperately requiring a solution. Only 9% of all plastic waste has been recycled, and whilst recycling gives second life to plastic, it costly there are limited downstream uses recycled therefore alternative urgently needed. Biodegradation by microorganisms developing field interest with the potential for bioreactors be used alongside degrade that may otherwise sent landfill. Here, we have identified two novel polyethylene...
Bacteria exist in polymicrobial environments and compete to prevail a niche. The type VI secretion system (T6SS) is nanomachine employed by Gram-negative bacteria deliver effector proteins into target cells. Consequently, T6SS-positive produce wealth of antibacterial promote their survival among prokaryotic community. These toxins are loaded onto the VgrG-PAAR spike Hcp tube T6SS apparatus recent work has started document specificity effectors for certain components. Pseudomonas aeruginosa...
Abstract Plastic pollution is an increasing worldwide problem urgently requiring a solution. While recycling rates are globally, only 9% of all plastic waste has been recycled, and with the cost limited downstream uses recycled plastic, alternative needed. Here, we found that expanded polystyrene (EPS) promoted high levels bacterial biofilm formation sought out environmental EPS to characterize these native communities. We demonstrated attached communities had degrading activity. then...
The gold standard method for the creation of gene deletions in Staphylococcus aureus is homologous recombination using allelic exchange plasmids with a temperature-sensitive origin replication. A knockout vector that contains regions homology first integrated into chromosome S. by single crossover event selected at high temperatures (non-permissive plasmid replication) and antibiotic selection. Next, second encouraged growth without selection low temperature, leading certain frequency to...
The type VI secretion system (T6SS) is a bacterial nanoscale weapon that delivers toxins into prey ranging from bacteria and fungi to animal hosts. cytosolic contractile sheath of the wraps around stacked hexameric rings Hcp proteins, which form an inner tube. At tip this tube puncturing device comprising trimeric VgrG topped by monomeric PAAR protein. number single per firing event remains unknown, since effectors can be loaded on diverse sites T6SS apparatus, notably device. Each or bind...
Pseudomonas aeruginosa is an opportunistic pathogen that can cause severe respiratory infections in people who are immunocompromised. P. possesses the Type VI Secretion System (T6SS), a bacterial weapon injects effectors into neighbouring prokaryotes and eukaryotes. The T6SS crucial for warfare, allowing to kill its competitors, which promotes dominance mixed microbial environments. has three T6SSs, H1/2/3-T6SS, these structural homologs but deliver unique effectors. Effectors delivered via...