A5006743069- Zebrafish Biomedical Research Applications
- Immune Response and Inflammation
- Cytokine Signaling Pathways and Interactions
- Immune Cell Function and Interaction
- Aquaculture disease management and microbiota
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Adipose Tissue and Metabolism
- Epigenetics and DNA Methylation
- Microplastics and Plastic Pollution
- Neuroscience and Neuropharmacology Research
- Blood disorders and treatments
- Calpain Protease Function and Regulation
- High Altitude and Hypoxia
- Immune cells in cancer
- Graphene and Nanomaterials Applications
- Acute Myeloid Leukemia Research
- Exercise and Physiological Responses
- NF-κB Signaling Pathways
- PI3K/AKT/mTOR signaling in cancer
- Effects and risks of endocrine disrupting chemicals
- Protein Kinase Regulation and GTPase Signaling
- Cellular transport and secretion
- Venomous Animal Envenomation and Studies
- Genetic factors in colorectal cancer
- interferon and immune responses
Deakin University
2015-2024
Karolinska Institutet
2022
Amrita Vishwa Vidyapeetham
2017
The Janus kinase (JAK) family is a small group of protein tyrosine kinases that represent central component intracellular signaling downstream from myriad cytokine receptors. JAK3 member performs particularly important role in facilitating signal transduction for key set receptors are essential immune cell development and function. Mutations impact activity have been identified number human diseases, including somatic gain-of-function (GOF) mutations associated with malignancies germline...
Excitotoxicity, a neuronal death process in neurological disorders such as stroke, is initiated by the overstimulation of ionotropic glutamate receptors. Although dysregulation proteolytic signaling networks critical for excitotoxicity, identity affected proteins and mechanisms which they induce cell remain unclear. To address this, we used quantitative N-terminomics to identify modified proteolysis neurons undergoing excitotoxic death. We found that most proteolytically processed are likely...
Granulocyte colony-stimulating factor receptor (G-CSFR), encoded by the CSF3R gene, represents a major regulator of neutrophil production and function in mammals, with inactivating extracellular mutations identified cohort neutropenia patients unresponsive to G-CSF treatment. This study sought elucidate role zebrafish G-CSFR generating mutants harboring these using genome editing.
The IL-2 family of cytokines act via receptor complexes that share the interleukin-2 gamma common (IL-2Rγc) chain to play key roles in lymphopoiesis. Inactivating IL-2Rγc mutations results severe combined immunodeficiency (SCID) humans and other species. This study sought generate an equivalent zebrafish SCID model. il2rga gene was targeted for genome editing using TALENs presumed loss-of-function alleles analyzed with respect immune cell development impacts on intestinal microbiota tumor...
The IL-2 receptor γ common (IL-2Rγc) chain is the shared subunit of receptors for family cytokines, which mediate signaling through JAK3 and various downstream pathways to regulate lymphopoiesis. Inactivating mutations in human IL-2Rγc result SCID, a primary immunodeficiency characterized by greatly reduced numbers lymphocytes. This study used bioinformatics, expression analysis, gene ablation, specific pharmacologic inhibitors investigate function two putative zebrafish paralogs, il-2rγc.a...
Background: Colony-stimulating factor 3 (CSF3), more commonly known as granulocyte colony-stimulating (G-CSF), acts via a specific cell surface receptor CSF3R (or G-CSFR) to regulate hematopoiesis, with particularly key role in the myeloid lineage where it impacts development and function of neutrophilic granulocytes. Zebrafish possess conserved homologue, Csf3r, which is involved both steady-state emergency myelopoiesis, well regulating early migration. Two CSF3 proteins have been...
JAK3 is principally activated by members of the interleukin-2 receptor family and plays an essential role in lymphoid development, with inactivating mutations causing autosomal-recessive severe combined immunodeficiency (SCID). This study aimed to generate equivalent zebrafish model SCID characterize across life-course. Genome editing jak3 created mutants similar those observed human SCID. Homozygous showed reduced embryonic T lymphopoiesis that continued through larval stage into adulthood,...
Cytokine-inducible SH2 domain-containing protein (CISH) is a member of the suppressor cytokine signaling (SOCS) family negative feedback regulators shown to play crucial roles in lymphoid cell development and function as well appetite regulation. It has also been implicated control downstream receptors for cytokines granulocyte/macrophage colony-stimulating factor (GM-CSF) granulocyte (G-CSF) myeloid cells. To investigate physiological role CISH myelopoiesis, mice deficient were analyzed...
Older adults experience chronic dysregulation of leukocytes and inflammatory cytokines, both at rest in response to resistance training. Systemic hypoxia modulates therefore this study characterized the effects normobaric on leukocyte cytokine responses older
Janus kinase 3 (JAK3) acts downstream of the interleukin-2 (IL-2) receptor family to play a pivotal role in regulation lymphoid cell development. Activating JAK3 mutations are associated with number and other malignancies, within regulatory pseudokinase domain common.The A572V A573V were separately introduced into highly conserved zebrafish Jak3 transiently expressed lines embryos examine their activity impact on early T cells. Genome editing was subsequently used introduce mutation genome...
Members of the FOS protein family regulate gene expression responses to a multitude extracellular signals and are dysregulated in several pathological states. Whilst mouse genetic models have provided key insights into tissue-specific functions these proteins vivo, little is known about their roles during early vertebrate embryonic development. This study examined potential using zebrafish as model for such studies and, more broadly, investigating mechanisms regulating Fos vivo. Through...
Ageing causes a decline in leukocyte function and blunted responses to resistance exercise. Systemic hypoxia exposure augments the response exercise young adults, yet this remains uncharacterised older adults. This study characterised effects of normobaric on acute inflammatory cytokine We recruited 20 adults aged 60-70 years perform an bout (FiO2 14.4%; n = 10) or normoxia 20.93%; 10). Participants completed 4 × 10 repetitions lower upper body exercises at 70% their predicted 1-repetition...
The study of host-pathogen interactions has improved our understanding both pathogenesis and the response host to infection, including innate adaptive responses. Neutrophils macrophages represent first line defense against any infection. zebrafish is an ideal model these cells a variety pathogens. Zebrafish possess neutrophils exhibiting similar mechanisms their human counterparts. transparency embryos greatly facilitates in vivo tracking infection dynamics non-invasive manner at...
Abstract Excitotoxicity is a neuronal death process initiated by over-stimulation of ionotropic glutamate receptors. Although dysregulation proteolytic signaling networks critical for excitotoxicity, the identity affected proteins and mechanisms which they induce cell remain unclear. To address this, we used quantitative N-terminomics to identify modified proteolysis in neurons undergoing excitotoxic death. We found that most proteolytically processed are likely substrates calpains,...
Bisphenol A (BPA) is an endocrine disrupter present in many daily products including food and beverage packaging. BPA exposure has been linked to disorders humans cancer, neurological disorders, diabetes obesity therefore declared toxic a partial ban effect several countries. Recently the plastic industry come with bisphenol analog as substitute for free products: BPS. However date, little known regarding toxicity of BPS vivo . In this study, we used zebrafish vertebrate model understand...
Introduction The granulocyte colony-stimulating factor receptor (G-CSFR), encoded by the CSF3R gene, is involved in production and function of neutrophilic granulocytes. Somatic mutations leading to truncated G-CSFR forms are observed acute myeloid leukemia (AML), particularly those subsequent severe chronic neutropenia (SCN), as well a subset patients with other leukemias. Methods This investigation introduced equivalent into zebrafish csf3r gene via genome editing used range molecular...