A5016558909- Cancer Immunotherapy and Biomarkers
- Herpesvirus Infections and Treatments
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Neonatal skin health care
- Parkinson's Disease Mechanisms and Treatments
- Whipple's Disease and Interleukins
- Child Nutrition and Feeding Issues
- Digestive system and related health
- Gut microbiota and health
- Tryptophan and brain disorders
- Immune cells in cancer
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- COVID-19 Clinical Research Studies
- Helicobacter pylori-related gastroenterology studies
- Cytomegalovirus and herpesvirus research
- Inflammatory Bowel Disease
- T-cell and B-cell Immunology
- Infectious Encephalopathies and Encephalitis
- Escherichia coli research studies
- Cystic Fibrosis Research Advances
- Yersinia bacterium, plague, ectoparasites research
- Retinal and Optic Conditions
Hôpital Maisonneuve-Rosemont
2020-2025
Université de Montréal
2020-2025
McGill University
2024-2025
Research Network (United States)
2024
Russian Medical Academy of Continuous Professional Education
2019
Ministry of Health
2015
Abstract Background The intestinal microbiome educates the immune system, in part through signaling antigen- presenting cells including dendritic (DCs). Germ-free mice exhibit DC deficiency, migration impairment, and priming dysfunction, implying a relationship between microbiota function. Naturally acquired, endemic Helicobacter species (H. spp.) such as H. hepaticus are common many mouse colonies, whereas some facilities Helicobacter-free. Aims Although they do not cause overt disease most...
Abstract Background Intestinal epithelial cells (IECs) provide an essential physical barrier between luminal contents and host tissue. Dysregulation of IECs leads to dysfunction, causing pathologies in both intestinal extra-intestinal diseases. While Parkinson’s Disease (PD) is primarily a neurodegenerative disorder, increasing evidence links PD progression gastrointestinal dysfunction. Our group developed model investigate the role gut PD, demonstrating that mice with genetic ablation...
Abstract Background The gut-brain axis is an area of intense interest in Parkinson’s disease (PD) research. PD characterized by inflammation and gastrointestinal (GI) dysfunction, such as recurrent constipation, dysphagia, intestinal bacterial overgrowth. GI symptoms appear decades before motor symptoms. Mutations the protein leucine-rich repeat kinase 2 (LRRK2) are associated with increased risk for inflammatory bowel disease. Among them, LRRK2 Gly2019Ser most common PD-associated mutation....
While immune checkpoint inhibitor therapy has transformed cancer treatment, the signaling pathways underlying T-cell function optimization remain incompletely understood. glycogen synthase kinase-3 (GSK-3) is a negative regulator and CD4+ T-cells provide help for CD8+ development, events controlling CD4-CD8 cooperation are unclear. Here, using GSK-3 knock-down mice (GSK-3KD), we show that central of cooperation, specifically in enhancing expression granzymes necessary CTL killing. GSK-3KD...
Purpose: To outline preparation of inoculum and per os Citrobacter rodentium infection in mice.
Current therapies to treat coronavirus disease 2019 (COVID-19) involve vaccines against the spike (S) protein S1 of SARS-CoV-2. Here, we outline an alternative approach involving chimeric antigen receptors (CARs) in T-cells (CAR-Ts). CAR-T recognition SARS-CoV-2 (RBD) peptide induced ribosomal S6 phosphorylation, increased expression activation antigen, CD69 and effectors, interferon-γ, granzyme B, perforin Fas-ligand on overlapping subsets CAR-Ts. CAR-Ts further showed potent vitro killing...
Abstract COVID-19, caused by SARS-CoV-2, can lead to a severe inflammatory disease characterized significant lymphopenia. However, the underlying cause for depletion of T-cells in COVID-19 patients remains incompletely understood. In this study, we assessed presence different T-cell subsets progression from mild disease, with focus on TCF1 expressing progenitor that are needed replenish peripheral during infection. Our results showed preferential decline TCF1+ CD4 and CD8+ severity. This was...
Immunotherapy using checkpoint blockade (ICB) with antibodies such as anti-PD-1 has revolutionised the treatment of many cancers. Despite its use to treat COVID-19 patients and autoimmune diseases systemic lupus erythematosus rheumatoid arthritis, effect hydroxychloroquine (HCQ) on cancer immunotherapy not been examined. In this study, remarkably, we find that HCQ alone, or in combination azithromycin (AZ), at doses used patients, decreased therapeutic benefit immunotherapy. No deleterious...
Abstract Parkinson’s disease (PD) is a chronic neurodegenerative disorder characterized by progressive loss of motor function. Diagnosis occurs late: after symptom development downstream the irreparable large proportion dopaminergic neurons in substantia nigra brain. Understanding PD pathophysiology its pre-motor prodromal phase needed for earlier diagnosis and intervention. Genetic risk factors, environmental triggers, dysregulated immunity have all been implicated development. Here, we...
Abstract Parkinson’s disease (PD) is characterized by a protracted period of non-motor symptoms, including gastrointestinal (GI) dysfunction, which can precede the development cardinal motor deficits decades. This long prodrome highly suggestive immune cell involvement in initiation disease, but currently field lacks robust model systems to study such mechanisms. It has been hypothesized that pathology may be first initiated periphery due environmental triggers, as pathogens enter GI tract....
Varicella Zoster Virus (VZV) is a highly contagious viral agent of the Herpesviridae family, which has strict species specificity, and causes two different diseases — chickenpox, mainly in children, herpes zoster more often elderly. Obtaining additional information about life cycle virus, its biology, pathogenetic features complications caused by it, will contribute to emergence advanced methods diagnosis prevention, development new experimental approaches that allow study innate adaptive...
We made recombinant antigen GE containing fragment of VZV glycoprotein E (Gly48 - Glu135) fused to E. coli beta-galactosidase and confirmed its specificity by Western blotting competitive-inhibition enzyme immunoassay (EIA) in comparison with commercial analogues natural viral antigens. showed interaction protein IgG antibodies from rabbits immunized vaccine strain. also specifically reacted ELISA 66% sera zoster patients 35% control groups including other herpes viruses, healthy donors,...
Abstract Background Intestinal epithelial cells (IECs) provide an essential physical barrier between harsh luminal contents and underlying host tissue. The maintenance of intestinal homeostasis must be intricately regulated through the proliferation differentiation stem (ISCs). Dysregulation this system results in loss function, causing pathologies both extra-intestinal diseases. While Parkinson’s Disease (PD) is primarily a neurodegenerative disorder, there increasing evidence linking PD...
Abstract Background Parkinson’s disease (PD) is a chronic neurodegenerative disorder. Several genetic predispositions, including the PTEN-induced kinase 1 (PINK1) mutation, have been implicated in early onset family cases. Besides loss of dopaminergic neurons brain, PD patients unique immune phenotype that includes increased inflammation, blood serum, brain levels proinflammatory cytokines, infiltration with cytotoxic CD8 T cells, and regulatory cells (Treg) their anti-inflammatory...
Our group has developed GI-targeted pathogen-induced PD mouse modeling systems (in PINK1 KO mice with gram negative bacterial infections) and found that T cells are a major player in driving PD-like motor symptoms at late stages following infection. Herein, we now map the initiating immune events site of infection earliest goal shedding light on mechanisms triggering cell-mediated pathological processes relevant to PD. Using unbiased single cell sequencing, demonstrate myeloid dysregulated...
This protocol details the Mitochondrial Antigen Presentation (MitAP) to 2CZ CD8+ T cell hybridoma. Previously, quantification of number IL-2 producing 2cz hybridoma cells in an ELISPOT assay was used as a readout for MitAP (Matheoud et al., 2016). Here, we adapted detection activation-induced markers (AIM) on with flow cytometry.
<title>Abstract</title> Parkinson’s disease (PD) is characterized by a protracted period of non-motor symptoms, including gastrointestinal (GI) dysfunction, which can precede the development cardinal motor deficits decades. This long prodrome highly suggestive immune cell involvement in initiation disease, but currently field lacks robust model systems to study such mechanisms. It has been hypothesized that pathology may be first initiated periphery due environmental triggers, as pathogens...
This protocol details the mitochondrial antigen presentation (MitAP) to primary 2C CD8 T (proliferation and suppression) assay. In order assess MitAP in an assay that incorporated all signals for naïve cells activation vivo, we tested whether H.pylori-exposed BMDCs could trigger of mitochondria-reactive cell.
This protocol details the extraction of brain infiltrating leukocytes (BILs).
Parkinson Disease (PD) is a progressive, neurodegenerative disorder characterised by motor and non-motor symptoms. Emerging evidence suggests link between PD gastrointestinal dysfunction. Constipation frequently observed years prior to development of dysfunction in PD, people with inflammatory bowel disease (IBD) are more likely develop PD. Mutations the leucine-rich repeat kinase 2 gene (LRRK2) account for approximately 1% all cases associated increased risk IBD. Among them, LRRK2...
Our group has developed GI-targeted pathogen-induced PD mouse modeling systems (in PINK1 KO mice with gram negative bacterial infections) and found that T cells are a major player in driving PD-like motor symptoms at late stages following infection. Herein, we now map the initiating immune events site of infection earliest goal shedding light on mechanisms triggering cell-mediated pathological processes relevant to PD. Using unbiased single cell sequencing, demonstrate myeloid dysregulated...
Our group has developed GI-targeted pathogen-induced PD mouse modeling systems (in PINK1 KO mice with gram negative bacterial infections) and found that T cells are a major player in driving PD-like motor symptoms at late stages following infection. Herein, we now map the initiating immune events site of infection earliest goal shedding light on mechanisms triggering cell-mediated pathological processes relevant to PD. Using unbiased single cell sequencing, demonstrate myeloid dysregulated...