A5000910150- Enzyme Catalysis and Immobilization
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Microbial Metabolites in Food Biotechnology
- Polysaccharides and Plant Cell Walls
- Polysaccharides Composition and Applications
- Agricultural and Food Sciences
- Digestive system and related health
- Microbial Metabolic Engineering and Bioproduction
- Electrochemical sensors and biosensors
- Phytochemicals and Antioxidant Activities
- Proteins in Food Systems
- Enzyme-mediated dye degradation
- Botanical Research and Applications
- Fermentation and Sensory Analysis
- Salmonella and Campylobacter epidemiology
- Microencapsulation and Drying Processes
- Advanced Nanomaterials in Catalysis
- Materials Engineering and Processing
- Parathyroid Disorders and Treatments
- Horticultural and Viticultural Research
- Listeria monocytogenes in Food Safety
- Genetic and Kidney Cyst Diseases
- Plant Gene Expression Analysis
- Water Quality Monitoring and Analysis
Universidade Federal de Ciências da Saúde de Porto Alegre
2017-2023
Hochschule Osnabrück
2019
Benemérita Universidad Autónoma de Puebla
2019
Universidade Federal do Rio Grande do Sul
2009-2016
Biocat
2016
Institute of Food Science & Technology
2016
Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Sul
2009-2015
Universidade Federal de Santa Catarina
2012-2015
Universidade Federal do Rio Grande
2013
In order to develop safer processes for the food industry, we prepared a chitosan support with naturally occurring crosslinking reagent, genipin, enzyme. As application model, it was tested immobilization of β-d-galactosidase from Aspergillus oryzae. Chitosan particles were obtained by precipitation followed adsorption enzyme and genipin. The characterized Fourier transform infrared (FTIR) spectroscopy thermogravimetric analysis (TGA). genipin provided biocatalysts satisfactory activity...
The effect of the support size on properties enzyme immobilization was investigated by using chitosan macroparticles and nanoparticles. They were prepared precipitation ionotropic gelation, respectively, characterized Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC), transmission electron microscopy (TEM), light scattering analysis (LSA), N2 adsorption–desorption isotherms. β-Galactosidase used as a model enzyme. It found that different sizes porosities...
β-D-Galactosidase from Kluyveromyces lactis was immobilized on glutaraldehyde-activated chitosan and used in a packed-bed reactor for the continuous hydrolysis of lactose synthesis galactooligosaccharides (GOS). The biocatalyst tested its optima pH temperature, thermal stability presence substrate products, operational stability. Immobilization increased range enzyme sharply lactose. Almost complete achieved both milk whey solution at 37 °C flow rates up to 2.6 mL min(-1). Maximal GOS...
The enzymatic synthesis of fructooligosaccharides (FOS) was carried out using a partially purified β-fructofuranosidase from the commercial enzyme preparation Viscozyme L. Partial purification L done by batch adsorption ion-exchange resin DEAE-Sepharose, showing 6-fold increase in specific activity. biocatalyst then covalently immobilized on glutaraldehyde-activated chitosan particles. Thermal stability evaluated at 50 °C and 60 °C, being around 100 times higher when compared to free enzyme....
Combi-CLEAs of pectinases and cellulases were prepared for grape juice clarification.
In the present study, we prepared two different magnetic biocatalysts of pectinase and cellulase: carrier-free CLEAs (CLEA-MP*) immobilization on glutaraldehyde-activated magnetite (Enz-Glu-MP*). The were compared to their properties, parameters, stability grape juice clarification. Enz-Glu-MP* presented higher properties than CLEA-MP*, whereas this surface area pore volume. KM enzyme immobilized was 25.65 mM, lower in comparison CLEA-MP* (33.83 mM). On other hand, most active stable...
Invertase (E.C.3.2.1.26) from Saccharomyces cerevisiae was covalently immobilized on chitosan nanoparticles prepared by the ionotropic gelation method and activated with glutaraldehyde. The support characterized it studied its load capacity, influence of presence substrate during immobilization, determined biocatalyst kinetic parameters stabilities. light scattering analysis (LSA) transmission electron microscopy (TEM) techniques indicated a mixture nano aggregated nanoparticles, providing...
Cyclodextrin glycosyltransferase (CGTase) from Thermoanaerobacter sp. was covalently immobilized on glutaraldehyde-activated chitosan spheres and used in a packed bed reactor to investigate the continuous production of β-cyclodextrin (β-CD). The optimum temperatures were 75 °C 85 at pH 6.0, respectively for free CGTase, (5.0) same both 60 °C. In reactor, effects flow rate substrate concentration β-CD evaluated. 4% (w/v), maximizing (1.32 g/L) 3 mL/min. addition, biocatalyst had good...
Enzyme-assisted extraction in liquid-liquid two-phase aqueous system was applied for the first time order to extract genipin from genipap. The effect of different commercial enzymes, their concentrations, and parameters were investigated. Moreover, chitosan gels prepared, crosslinked with glutaraldehyde or characterized by textural rheological properties. used as support immobilization model β-galactosidases. Among enzymes tested extraction, Celluclast 10% (36 °C pH 3.7) provided an 196...
Magnetic-chitosan particles were prepared following three different protocols enabling the preparation of with sizes – nano (Nano-CMag, Micro (Micro-CMag) and Macro (Macro-CMag) used for pectinase immobilization clarification grape, apple orange juices. The particle size had a great effect in kinetic parameters, Nano-CMag biocatalyst presented highest Vmax value (78.95 mg. min−1), followed by Micro-CMag Macro-CMag, 57.20 mg.min−1 46.03 mg.min−1, respectively. However, thermal stability was...
In this work, we studied the effect of some variables in reaction between genipin and chitosan, to prepare a support for enzyme immobilization. Reaction temperature, pH, time concentration were evaluated activation β-galactosidase from Aspergillus oryzae was used as model. The immobilized characterized by immobilization properties kinetic parameters thermal operational stability. It found that at alkaline pH (9.0), 60 °C 1 h, resulted an activity recovery 37.5%. had stabilization factor 2.0...
In this work, the synergistic effects of combination Pectinex Ultra Clear (PUC), presenting high pectinase activity, and Lallzyme Beta (LB), cellulase activity were tested for grape juice extraction from Vitis labrusca L. variety Concord. The variables time, temperature, enzyme concentration PUC/LB ratio analyzed with responses yield reducing capacity. PUC improved while LB increased However, mix provided an increase in capacity, reaching to 75.8% 1090 mg L−1, respectively. best conditions...
Wasted bread (WB) has been studied as an alternative ingredient for increasing the sustainable footprint in beer production chain. There are gaps literature on impact of WB manufacturing. Thus, objective was to evaluate addition a replacement wheat flakes craft beer. Three formulations with different concentrations were produced and monitored glucose maltose mash; analyzed ethanol, glycerol, acetic acid, lactic pH, acidity, turbidity, color, volatile compounds. Sensory analysis performed by...
Thin films containing silver nanoparticles homogeneously dispersed, with narrow size distribution below 10 nm, were synthesized on flat glass surface, by using an ionic silsesquioxane as stabilizer and crosslinking agent.The can be prepared without previous functionalization of substrate surfaces addition other components.The heat treated up to 200 ºC characterized ultraviolet-visible, transmission electron microscopy, atomic force thermogravimetric analysis ellipsometry.The thermally stable...