A5041756517- Cell Adhesion Molecules Research
- Osteoarthritis Treatment and Mechanisms
- Mass Spectrometry Techniques and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Inflammatory mediators and NSAID effects
- thermodynamics and calorimetric analyses
- Microfluidic and Capillary Electrophoresis Applications
- RNA Research and Splicing
- Bone and Dental Protein Studies
- Collagen: Extraction and Characterization
- Wnt/β-catenin signaling in development and cancer
- Analytical Chemistry and Chromatography
- CAR-T cell therapy research
- Virus-based gene therapy research
Massachusetts Institute of Technology
2020-2024
Purdue University West Lafayette
2017-2019
State Street (United States)
2019
Center for Cancer Research
2019
An integrated system utilizing mass spectrometry for reaction screening, continuous-flow optimization, and continuous crystallization of diphenhydramine has been developed.
Abstract We demonstrate the use of accelerated reactions with desorption electrospray ionization mass spectrometry (DESI-MS) as a tool for predicting outcome microfluidic reactions. DESI-MS was employed high throughput experimentation to provide qualitative predictions reaction outcomes, so that vast regions chemical reactivity space may be more rapidly explored and areas optimal efficiency identified. This work is part larger effort accelerate optimization enable rapid development...
Collagens are the foundational component of diverse tissues, including skin, bone, cartilage, and basement membranes, most abundant protein class in animals. The fibrillar collagens large, complex, multidomain proteins, all containing characteristic triple helix motif. prevalent heterotrimeric, meaning that cells express at least two distinctive procollagen polypeptides must assemble into specific heterotrimer compositions. molecular mechanisms ensuring correct heterotrimeric assemblies...
Collagenopathies are a group of clinically diverse disorders caused by defects in collagen folding and secretion. For example, mutations the gene encoding type-II, primary cartilage, can lead to chondrodysplasias. One example is Gly1170Ser substitution procollagen-II, which causes precocious osteoarthritis. Here, we biochemically mechanistically characterize an induced pluripotent stem cell-based cartilage model this disease, including both hetero- homozygous genotypes. We show that...
To develop an in vitro disease model of a human chondrodysplasia, we used CRISPR/Cas9 gene editing to generate heterozygous COL2A1 exon 50 c.3508 GGT > TCA (p.G1170S) mutation control iPSC line. Both the and mutant lines displayed typical characteristics, including normal cell morphology, expression pluripotency markers, ability differentiate into endoderm, ectoderm mesoderm lineages karyotype. These chondrodysplasia isogenic can be explore mechanisms underlying type II collagenopathies aid...
The human iPSC line MCRIi019-A-6 was generated using CRISPR/Cas9-mediated gene editing to introduce a heterozygous COL2A1 exon 33 c.2155C>T (p.R719C) mutation into the control MCRIi019-A. Both edited and parental lines display typical characteristics, including expression of pluripotency markers, ability be differentiated three germ lines, normal karyotype. This cell line, along with isogenic can used study molecular pathology precocious osteoarthritis in model, more broadly understand type...
ABSTRACT Microbial respiration alone releases massive amounts of Carbon (C) into the atmosphere each year, greatly impacting global C cycle that fuels climate change. Larger microbial population growth often leads to larger standing biomass, which in turns higher respiration. How rising temperatures might influence growth, however, depends on how thermal performance curves (TPCs) governing this may adapt novel environments. This adaptation will turn depend there being heritable genetic...
ABSTRACT Objectives Mutations in the procollagen-II gene ( COL2A1 ) often cause chondrodysplasias, including precocious osteoarthritis-inducing p.Arg719Cys. Understanding molecular basis of such diseases has long been challenging, owing to a lack models accurately reflecting disease genotypes and phenotypes. To address this challenge, we develop characterize vitro human cartilage derived from wild-type disease-causing Arg719Cys isogenic induced pluripotent stem cell (iPSC) lines. Methods...
Abstract Collagenopathies are a group of clinically diverse disorders caused by defects in collagen folding and secretion. For example, mutations the gene encoding type-II, primary cartilage, can lead to chondrodysplasias. One example is Gly1170Ser substitution procollagen-II, which causes precocious osteoarthritis. Here, we biochemically mechanistically characterize an induced pluripotent stem cell-based cartilage model this disease, including both hetero- homozygous genotypes. We show that...