A5044109042- Zebrafish Biomedical Research Applications
- Veterinary Equine Medical Research
- Digestive system and related health
- Insect Utilization and Effects
- Lysosomal Storage Disorders Research
- Animal and Plant Science Education
- Aquaculture Nutrition and Growth
- Fatty Acid Research and Health
- Reproductive Physiology in Livestock
- Animal health and immunology
- Muscle metabolism and nutrition
- Animal Diversity and Health Studies
- Infant Nutrition and Health
- Milk Quality and Mastitis in Dairy Cows
- Aquaculture disease management and microbiota
- Genetics and Neurodevelopmental Disorders
- Ruminant Nutrition and Digestive Physiology
- Glycogen Storage Diseases and Myoclonus
- Cholesterol and Lipid Metabolism
- Autophagy in Disease and Therapy
- Cannabis and Cannabinoid Research
- Retinopathy of Prematurity Studies
- Spinal Cord Injury Research
- Mitochondrial Function and Pathology
- Food, Nutrition, and Cultural Practices
University of Pisa
2017-2025
Fondazione Stella Maris
2021-2025
Central Luzon State University
2023
Istituti di Ricovero e Cura a Carattere Scientifico
2021
Biallelic mutations in the SACS gene, encoding sacsin, cause early-onset autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS), a neurodegenerative disease also characterized by unique and poorly understood retinal abnormalities. While two murine models replicate phenotypic neuronal features observed patients, no phenotype has been described so far. In zebrafish knock-out strain that faithfully mirrors main aspects ARSACS, we impaired visual function due to photoreceptor...
The impact of enrichment on stress reduction in zebrafish (Danio rerio) exposed to a novel environment was assessed. Four control shoals (CTRL) and five treated (TRT), each with eight fish, were observed; TRT tanks, PVC pipe included (three-way tube, 11.7 × 4 cm) as for 90 days. Subsequently, fish moved new tank shoaling test, behavior evaluated over periods 0'-5' 5'-10'. Cortisol dissolved water measured before after the test. No differences found between two groups distance moved, swimming...
Mutations in the EPM2A gene encoding laforin cause Lafora disease (LD), a progressive myoclonic epilepsy characterized by drug-resistant seizures and neurological impairment. To date, rodents are only available models for studying LD; however, their use drug screening is limited regulatory restrictions high breeding costs. investigate role of loss function early neurodevelopment, to screen possible new compounds treating disorder, we developed zebrafish model LD. Our results showed epm2a-/-...
Autism spectrum disorder (ASD) is a multifactorial neurodevelopmental condition with several identified risk factors, both genetic and non-genetic. Among these, prenatal exposure to valproic acid (VPA) has been extensively associated the development of disorder. The zebrafish, cost- time-effective model, useful for studying ASD features. Using validated VPA-induced zebrafish models, we aimed provide new insights into VPA effects during embryonic identify potential biomarkers ASD-like...
Abstract CLN5 disease, a form of juvenile dementia within the neuronal ceroid lipofuscinosis (NCL), is associated with mutations in gene encoding lysosomal bis(monoacylglycero)phosphate (BMP) synthase, essential for BMP production and function. Limited knowledge cellular mechanisms unclear drug targets hinder translating this to children’s treatment, which remains symptomatic. We developed characterized new cln5 knock-out zebrafish model that replicates key features molecular signatures...
CLN8 and other neuronal ceroid lipofuscinoses (NCLs) often lead to cognitive decline, emotional disturbances, social deficits, worsening with disease progression. Disrupted lysosomal pH, impaired autophagy, defective dendritic arborization contribute these symptoms. Using a cln8−/− zebrafish model, we identified significant impairments in locomotion, anxiety, aggression, along subtle deficits interactions, positioning as useful model for therapeutic studies NCL. Our findings show that...
<title>Abstract</title> Autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS<bold>)</bold> is an early-onset neurodevelopmental and neurodegenerative disorder characterized by ataxia, spasticity, peripheral neuropathy. However, several studies have highlighted that some patients also experience cognitive, emotional social deficits, suggesting a more complex clinical picture extends beyond motor symptoms. Building on these findings, this study aimed to: i) investigate locomotor,...
Vitamin D is an essential nutrient that plays a crucial role in calcium homeostasis and bone metabolism also acts as hormone. Although several studies on the content of vitamin bovine milk have been conducted, little information available regarding donkey milk. In context nutritional assessment milk, aim this study was to assess its chemical profile, with particular reference seasonal technological modifications after pasteurization. The conducted dairy farm produces for human consumption...
For improving aquafeed sustainability, insect meal is currently considered the most promising alternative to fishmeal. However, in this regard, more data are still necessary avoid possible negative impacts on fish growth performance, metabolism, and welfare. The present study investigated effects of increasing inclusion Hermetia illucens (0%, 17%, 33% 50% feed, equating 0%, 34%, 66% 100% fishmeal replacement) mortality, intestine morphology, gene expression intestinal carriers. results...
CLN8 is an endoplasmic reticulum cargo receptor and a regulator of lysosome biogenesis whose loss function leads to neuronal ceroid lipofuscinosis. has been linked autophagy lipid metabolism, but much remains be learned, there are no therapies acting on the molecular signatures in this disorder. The present study aims characterize pathways involved disease and, by pinpointing altered ones, identify potential therapies. To bridge gap between cell mammalian models, we generated new zebrafish...
The current study evaluated the effects of hydrolyzable and condensed tannins from chestnut quebracho wood, respectively (TSP, Silvafeed®), on zebrafish with intestinal inflammation induced by a plant-based diet (basal diet). Four experimental diets were prepared as follows: basal + 0 TSP, TSP at 0.9 g/kg feed, 1.7 3.4 feed. Eighty-four (Danio rerio) fed for 12 days diets. In diet, intestine integrity appeared to be altered, damaged villi, high immunoexpression tumor necrosis factor-α (TNFα)...
Fishmeal (FM) is still the most important protein source in aquafeeds. However, due to reduction of wild fish stocks used for FM production, its manufacturing it now unsustainable. Insect meal represents a valid alternative FM, low carbon footprint production and high nutritional value. The aim this study was investigate potentials replacing with black soldier fly (Hermetia illucens) (HIM) aquafeeds, using zebrafish as animal model. Four diets were formulated increasing HIM/FM replacement...
Autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS) is a multisystem hereditary associated with mutations in SACS, which encodes sacsin, protein still only partially understood function. Although mouse models ARSACS mimic largely the disease progression seen humans, their use validation effective therapies has not yet been proposed. Recently, teleost Danio rerio attracted increasing attention as vertebrate model that allows rapid and economical screening, candidate molecules,...
Donkey milk is known for some nutritional and nutraceutical peculiarities compared to the milks traditionally used human nutrition. Moreover although number of studies on donkey production has increased asinine species still remains little investigated. This first study providing a multiple assessment approach in order extend knowledge milk, haematological cytological parameters during whole span lactation. Furthermore, this characterised LYZ OXT genes. Twenty two individual blood samples...
Duchenne muscular dystrophy (DMD), caused by mutations in the dystrophin gene, is an inherited neuromuscular disorder that causes loss of muscle mass and motor skills. In era genomic medicine, there still no known cure for DMD. clinical practice, a growing awareness possible importance nutrition diseases. This mostly result patients’ or caregivers’ empirical reports how active substances derived from food have led to improved strength and, thus, better quality life. this report, we...
Duchenne muscular dystrophy (DMD) is a severe X-linked neuromuscular childhood disorder that causes progressive muscle weakness and degeneration. A lack of dystrophin in DMD leads to inflammatory response, autophagic dysregulation, oxidative stress skeletal fibers play key role the progression pathology. β-glucans can modulate immune function by modifying phagocytic activity immunocompetent cells, notably macrophages. Mitochondrial also involved an important mechanism innate adaptive...