A5014247752- Neurobiology and Insect Physiology Research
- Lepidoptera: Biology and Taxonomy
- Genetic diversity and population structure
- Invertebrate Immune Response Mechanisms
- Genetic Mapping and Diversity in Plants and Animals
- Developmental Biology and Gene Regulation
- Protist diversity and phylogeny
- Marine Invertebrate Physiology and Ecology
- Animal Behavior and Reproduction
- Genetic and phenotypic traits in livestock
George Washington University
2021-2023
ABSTRACT Butterfly color patterns provide visible and biodiverse phenotypic readouts of the patterning processes. Although secreted ligand WntA has been shown to instruct pattern formation in butterflies, its mode reception remains elusive. genomes encode four homologs Frizzled-family Wnt receptors. Here, we show that CRISPR mosaic knockouts frizzled2 (fz2) phenocopy effects loss function multiple nymphalids. Whereas clones result intermediate reduced size, fz2 are cell-autonomous,...
Continuous color polymorphisms can serve as a tractable model for the genetic and developmental architecture of traits. Here we investigated continuous variation in Colias eurytheme philodice, two species sulphur butterflies that hybridize sympatry. Using quantitative trait locus (QTL) analysis high-throughput quantification, found interacting large-effect loci affecting orange-to-yellow chromaticity. Knockouts red Malpighian tubules (red), likely involved endosomal maturation, result...
Abstract Butterfly color patterns provide visible and biodiverse phenotypic readouts of the patterning processes that occur in a developing epithelium. While secreted ligand WntA was shown to instruct pattern formation butterflies, its modes reception signal transduction remain elusive. genomes encode four homologues Frizzled-family Wnt receptors. Here we show CRISPR mosaic knock-outs frizzled2 ( fz2 ) phenocopy effects loss-of-function multiple nymphalids. clones result intermediate reduced...
Abstract Wnt ligands are key signaling molecules in animals, but little is known about the evolutionary dynamics and mode of action WntA orthologs, which not present vertebrates or Drosophila . Here we show that subfamily evolved at base Bilateria + Cnidaria clade, conserved thumb region Ser209 acylation site most other Wnts, suggesting requires core secretory pathway. proteins distinguishable from Wnts by a synapomorphic Iso/Val/Ala216 amino‐acid residue replaces otherwise ubiquitous Thr216...
Summary Continuous colour polymorphisms can serve as a tractable model for the genetic and developmental architecture of traits, but identification causative loci is complex due to number individuals needed, challenges scoring continuously varying traits. Here we investigated continuous variation in Colias eurytheme C. philodice , two sister species sulphur butterflies that hybridise sympatry. Using Quantitative Trait Locus (QTL) analysis 483 from interspecific crosses an high-throughput...