A5070503523- Enzyme Catalysis and Immobilization
- biodegradable polymer synthesis and properties
- Biofuel production and bioconversion
- Protein Hydrolysis and Bioactive Peptides
- Microbial Metabolic Engineering and Bioproduction
- Lignin and Wood Chemistry
- Enzyme Production and Characterization
- Catalysis for Biomass Conversion
- Polysaccharides Composition and Applications
- Nanocomposite Films for Food Packaging
- Chemical Synthesis and Analysis
- Microbial Metabolites in Food Biotechnology
- Food composition and properties
- Proteoglycans and glycosaminoglycans research
- Analytical Chemistry and Chromatography
- Glycosylation and Glycoproteins Research
- Polysaccharides and Plant Cell Walls
- Enzyme-mediated dye degradation
- Phytochemicals and Antioxidant Activities
- Potato Plant Research
- Postharvest Quality and Shelf Life Management
- Carbohydrate Chemistry and Synthesis
- Electrochemical sensors and biosensors
- Peptidase Inhibition and Analysis
- Ionic liquids properties and applications
Polytechnic University of Timişoara
1988-2025
Wageningen University & Research
2014-2025
Aurel Vlaicu University of Arad
1970-2014
Food & Nutrition
2001-2009
Nestlé (Switzerland)
2002
Findus (Sweden)
2002
European Organisation for Research and Treatment of Cancer
2000
Massachusetts Institute of Technology
1986
Natural deep eutectic solvents (NADESs) are a sustainable, green option for extraction and reaction media in biorefineries various chemical biotechnological applications. Particularly, enzymatic reactions profit from NADES applications, as these help to maintain high substrate solubility while improving both enzyme stability efficiency. Recent studies confirmed that NADESs can perform multiple functions simultaneously, biocatalytic conversions, but also substrates catalysts reactions,...
The antioxidant activity of caseins and casein-derived peptides was evaluated by using three free radical producing reactionsthe lipoxygenase- AAPH-catalyzed oxidation linoleic acid the hemoglobin-catalyzed hydroperoxide. Caseins were able to inhibit enzymatic nonenzymatic lipid peroxidation, suggesting they preferred targets for intermediates. antioxidative feature not lost with dephosphorylation or proteolysis proteins. fractionation tryptic β-casein digest yielded activity. A...
Hyaluronan is a polysaccharide with multiple functions in the human body being involved creating flexible and protective layers tissues many signalling pathways during embryonic development, wound healing, inflammation, cancer. an important component of active pharmaceutical ingredients for treatment of, example, arthritis osteoarthritis, its commercial value far exceeds that other microbial extracellular polysaccharides. Traditionally hyaluronan extracted from animal waste which...
Chitosan is a polysaccharide with recognized antioxidant, antimicrobial and wound healing activities. However, this polymer soluble only in dilute acidic solutions, which restricts much of its applications. A usual strategy for improving the functionality polysaccharides selective oxidation mediated by 2,2,6,6-tetra-methyl-1-piperidinidyloxy (TEMPO) using laccase as co-oxidant. In work, TEMPO-laccase redox system was used first time to selectively oxidize chitosan order produce tailored...
The tyrosine-containing peptide Gly-Tyr-Gly (GYG) was oxidatively cross-linked by horseradish peroxidase in the presence of hydrogen peroxide. As products, covalently coupled di- to pentamers were identified LC-MS. Oxidative cross-linking ferulic acid with and peroxide resulted formation dehydrodimers. Kinetic studies conversion rates either or revealed conditions that allow heteroadducts GYG acid. To a GYG-containing incubation mixture added small aliquots, therewith keeping molar ratio...
Whole casein from bovine origin, the different subtypes α, β, and κ, related dephosphorylated proteins were assayed as modulators of soybean lipoxygenase 1 activity found to inhibit it. To define inhibitory domain, whole β-casein digested by proteases (trypsin, clostripain, subtilisin). The tryptic digest subtilisin digests retained their properties. was most potent inhibitor further fractionated FPLC or HPLC. collected peptides inhibited lipoxygenase-catalyzed reaction extents. active...
A thermostable Chitinase Chi1 from Myceliophthora thermophila C1 was homologously produced and characterized. shows high thermostability at 40 °C (>140 h 90% activity), 50 (>168 55 (half-life 48 h). has broad substrate specificity converts chitin, chitosan, modified chitin oligosaccharides. The activity of is strongly affected by the degree deacetylation (DDA), molecular weight (Mw), side chain modification chitosan. releases mainly (GlcNAc)2 insoluble chito-oligosaccharides with a...
Abstract Deep eutectic solvents (DESs) advanced in the past decade as new generation of green and sustainable extraction reaction media biorefineries a multitude chemical biotechnological processes. Particularly, enzyme catalyzed reactions benefit from specific properties DESs, which not only ensure good substrate solubility but also enhance stability operational efficiency. Recent studies showed that natural deep (NADESs) comprising choline chloride or betaine hydrogen bond acceptor large...
An integrated strategy was followed to valorise rice straw, one of the most relevant biomass feedstocks available worldwide, selectively recover solubilised hemicelluloses and lignin.The pathway encompassed use autohydrolysis hydrolyse an ethanol-based organosolv process solubilise lignin.Several conditions were assayed with best results obtained at 210 ºC (log R0 4.15), which enabled high removal hemicelluloses, yielding oligosaccharide-rich hydrolysate a treated low content enriched in...
The capture and utilization of CO2 from industrial off-gases to produce commodity chemicals has the potential make an important contribution transition a circular economy. Formate, its conjugate formic acid, is sustainable platform chemical that can be produced CO2. Electrochemical reduction formate promising route, but biocatalysis with dehydrogenase (FDH) as biocatalyst may offer advantages for implementation such mild conditions, high product selectivity, less expensive down-stream...
Heterologous conjugates of wheat arabinoxylan and β-casein were prepared via enzymatic cross-linking, using sequential addition the to a mixture β-casein, peroxidase, hydrogen peroxide. The maximal formation adducts between feruloylated was reached at protein-to-arabinoxylan ratio 10:1, in combination with molar peroxide substrate 2:1 protein-to-enzyme 102 104. protein−arabinoxylan separated from homopolymers by size exclusion anion exchange chromatography. protein:arabinoxylan purified...
Abstract 2,5-Furandicarboxylic acid is a platform chemical for the production of biobased polymers and materials. This study reports synthesis furan oligoesters via polytransesterification dimethyl furan-2,5-dicarboxylate linear α, ω-aliphatic diols with chain length ranging from C2 to C12, using immobilized lipase B Candida antarctica (Novozym 435) in dry organic solvents. Dimethyl furan-2,5-dicarboxylic (A) 1,4-butanediol (B) were used as model substrates under different conditions...
Bioplastics may solve environmental issues related to the current linear plastic economy, but they need improvement be viable alternatives. To achieve this, we aimed add chitin nanocrystals (ChNC) polylactic acid (PLA), which is known alter material properties while maintaining a fully bio-based character. However, ChNC are not particularly compatible with PLA, and surface modification fatty acids was used improve this. We that different in carbon chain length (C4-C18) degree of saturation...
Lauroylcholine chloride is the product of lipase catalysed esterification a choline chloride/glucose/water (2 : 1 1) reactive natural deep eutectic solvent (R-NADES).