Abstract.— Responses of rainbow trout, Oncorhynchus mykiss , to handling and confinement stress were used validate the efficacy portable instruments for measuring blood glucose lactate, plasma protein. Glucose lactate increased with time showed good linear correlation after disturbance. The Advantage Accusport™ meters similarly correlated, except that values consistently lower than those obtained by established laboratory techniques. Refractometry gave higher protein results from biuret...

ABSTRACT Muscle metabolites in resting, tank acclimated snapper, Pagrus auratus , were monitored for 72 hr postmortem and compared with values from exercised or commercially caught fish. The physiological status of the live animal was quantified by plasma cortisol blood chemistry. Cortisol levels lowest unstressed controls (6.8 ± 2.1 ng mL −1 ) while laboratory fish had highest (67.7 11.2 ). Control maintained a constant K‐value chilled storage; all other groups significant increases. Onset...

The blood of seven Antarctic nototheniid species and representatives from three other families contained low haemoglobin concentrations compared with non‐polar marine teleosts. Haematocrit values were slightly lower than teleosts, while erythrocyte counts substantially reduced by comparison. Interspecific variation in concentration seemed to be a function activity level. Mean corpuscular all was strikingly comparison latitudes not correlated the habits species. Haemoglobin componentry using...

Physiologic findings obtained by cardiac catheterization on 29 patients with mitral valvular disease are discussed. Cardiac outputs, intracardiac pulmonary arterial and pressures determined during rest exercise. It is shown that the resting output reduced in all exercise results either a fall or small increase minute volume of circulation. The artery capillary elevated rise further A clear differentiation physiologic means between stenosis insufficiency difficult. However, lower, venous...

ABSTRACT The teleost spleen is a discrete organ containing, in addition to erythrocytes sequestered from the circulation, erythropoietic tissue involved synthesis of new erythrocytes. In this division into supply and synthesis, there appear be some species differences. eel an erythrocyte reservoir but not thought major (Johansson-Sjobeck, 1979). splenic mass goldfish does change with induced anaemia (Houston et al. 1988). trout spleen, contrast, plays role during (Lane, 1979), serving as...

Abstract Groups of rainbow trout ( Salmo gairdneri , Richardson) were continuously swum at 20 cm s −1 (1.0 body lengths ) for 0, 3, 30, and 200 days. No significant changes in fish condition factor, combined red white muscle mass, fibre size or distribution observed. After days swimming there was a 2.2 fold increase mass. Number capillaries per increased significantly each group by maximum 27% after exercise. 95% Blood lactate, haemoglobin (Hb) concentration haematocrit, erythrocyte...

The viscosity of blood from rainbow trout was measured following manipulation haematocrit by bleeding, hypoxia. exercise, and anaesthesia. Blood when at high shear rate (225 s 1 ) proportional to haematocrit, but the dependence on far less for swollen erythrocytes exercised anaesthetized trout. Erythrocyte swelling most marked in trout, is a confounding factor considering effect viscosity. with variable constant mean cell Hb concentration, indicated that relative oxygen transport capacity...

ABSTRACT The effects of hypoxic exposure on whole-blood oxygen-affinity were examined in the antarctic fish Pagothenia borchgrevinki. Fish exposed to for 11–14 days at −1·5°C had a P50 value 20·6 ± 4·8 mmHg (S.D., N =13) pH8-16, compared with 31·1 4·3mmHg (N = 10) pH8-00 normoxic fish. Exposure low oxygen levels resulted significant (66 %) rise haemoglobin concentration, and erythrocyte [ATP] decreased by approximately 27%. There was no evidence swelling. An aberrant gill morphology observed...

ABSTRACT The oxygenation-linked, allosteric interactions of erythrocytic organic phosphates and urea with the haemoglobin (Hb), functional significance Hb multiplicity, were studied in an elasmobranch, Squalus acanthias. autochthonous red cell nucleoside triphosphates (NTP) ATP GTP (guanosine triphosphate) strongly depress O2 affinity stripped (cofactor-free) increase cooperativity binding. As previously found teleost Hbs, exerts a greater effect than at same concentration. Urea, contrast,...

Three members of an Oxfordshire family have polycythaemia. In each case their whole-blood oxygen affinity is increased. This due to a previously undescribed haemoglobin variant which has been named Radcliffe (alpha2beta299(Gl)Ala). addition having high shows virtually no haem-haem interaction and diminished Bohr effect. It synthesized at the same rate as stable A. X-ray analysis indicates that crystals deoxyhaemoglobin are isomorphous with those Solutions were also studied by high-resolution...

The icefishes (family Channichthyidae) comprise a unique group of teleost fishes endemic to Antarctic and sub‐antarctic seas. All members the family totally lack haemoglobin. Haematological parameters viscosity were determined for blood from 11 specimens two channichthyid species ( Chionodraco kathleenae Regan, 1914; Cryodraco antarcticus Dollo, 1900), 14 red‐blood nototheniid Pagothenia bernacchii (Boulenger, 1902)), captured near Italian research station at Terra Nova Bay, Ross Sea,...

ABSTRACT Arterial and mixed venous blood were sampled through chronically implanted cannulae from rested swimming hagfish. remained high when hagfish swum for 15min at a velocity of 20cms-1. fell 17-2mmHg rest to 3’5 mmHg after swimming, the arteriovenous pH difference increased 0·15 0·25 units. Whole oxygen equilibrium curves essentially hyperbolic (Hill’s n value = 1·38) gave half-saturation (P50) 12·3 7·8 16°C. A CO2-Bohr factor (ϕ ΔlogP50/ΔpH) –0·43 limited buffering capacity blood,...

ABSTRACT The effects of thermal acclimation on whole blood oxygen affinity were examined in the antarctic fish Pagothenia borchgrevinki. 4·5 °C-acclimated had a P50 value 26·7 mmHg at pH 8·4, compared to 20·7 for −1·5 fish. apparent heat oxygenation, ΔH = −26·7 kJ mol−1, is comparable values temperate species. Warm-acclimation was followed by an increased ATP:Hb4 molar ratio, resulting augmentation effect oxy-haemoglobin affinity. This may be considered adaptive constantly well oxygenated...