A5057657756- interferon and immune responses
- Influenza Virus Research Studies
- SARS-CoV-2 and COVID-19 Research
- Bacterial Genetics and Biotechnology
- Mosquito-borne diseases and control
- SARS-CoV-2 detection and testing
- COVID-19 epidemiological studies
- Antibiotic Resistance in Bacteria
- Virus-based gene therapy research
- Antimicrobial Peptides and Activities
- Trace Elements in Health
- Viral Infections and Vectors
- Tryptophan and brain disorders
- RNA and protein synthesis mechanisms
- Viral Infections and Immunology Research
- Immune Cell Function and Interaction
- Neuroendocrine regulation and behavior
- Stress Responses and Cortisol
- Ubiquitin and proteasome pathways
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- Viral Infections and Outbreaks Research
- Clostridium difficile and Clostridium perfringens research
- Analytical Chemistry and Chromatography
- Metal Extraction and Bioleaching
Public Health Agency of Sweden
2014-2025
Lund University
2023
Linköping University
2023
Karolinska Institutet
2023
Swedish Armed Forces
2023
University of Basel
2018-2021
Finnish Institute of Occupational Health
2014-2017
University of Helsinki
2014-2017
Helsinki Institute of Physics
2016
Czech Academy of Sciences, Institute of Biotechnology
2016
Gram-negative bacterial pathogens have an outer membrane that restricts entry of molecules into the cell. Water-filled protein channels in membrane, so-called porins, facilitate nutrient uptake and are thought to enable antibiotic entry. Here, we determined role porins a major pathogen, Pseudomonas aeruginosa, by constructing strain lacking all 40 identifiable 15 strains carrying only single unique type porin characterizing these with NMR metabolomics antimicrobial susceptibility assays. In...
Staphylococcus aureus causes invasive infections and easily acquires antibiotic resistance. Even antibiotic-susceptible S. can survive therapy persist, requiring prolonged treatment surgical interventions. These so-called persisters display an arrested-growth phenotype, tolerate high concentrations, are associated with chronic recurrent infections. To characterize these persisters, we assessed recovered directly from a patient suffering persistent infection. We show that host-mediated...
Influenza A viruses cause infections in the human respiratory tract and give rise to annual seasonal outbreaks, as well more rarely dreaded pandemics. become quickly resistant virus-directed antiviral treatments, which are current main treatment options. promising alternative approach is target host cell factors that exploited by influenza viruses. To this end, we characterized phosphoproteome of virus infected primary macrophages elucidate intracellular signaling pathways critical activated...
The influenza pH1N1 virus caused a global flu pandemic in 2009 and continues manifestation as seasonal virus. Better understanding of the virus-host cell interaction could result development better prevention treatment options. Here we show that Akt inhibitor MK2206 blocks infection vitro. In particular, at noncytotoxic concentrations, alters signaling inhibits endocytic uptake Interestingly, is unable to inhibit H3N2, H7N9, H5N1 viruses, indicating evolved specific requirements for...
Background Dengue is a mosquito-borne viral disease that has become more prevalent in the last few decades. Most patients are viremic when they present with symptoms, and early diagnosis of dengue important preventing severe clinical complications associated this also represents key factor differential diagnosis. Here, we designed validated hydrolysis-probe-based one-step real-time RT-PCR assay targets genomes virus serotypes 1–4. Methodology/Principal Findings The primers probe used our...
Acute SARS-CoV-2 infections are not always diagnosed; hence an unknown proportion of all documented. can induce spike and nucleocapsid protein specific IgG antibodies, which be detected in seroprevalence studies to identify a previous infection. However, with the introduction vaccines containing it is no longer possible use spike-IgG as marker In many countries marketed do include protein, allowing nucleocapsid-specific (N-IgG) for Importantly however, SARS-CoV-2-infected individuals develop...
Copper, while toxic in excess, is an essential micronutrient all kingdoms of life due to its role the structure and function many proteins. Proteins mediating ionic copper import have been characterised detail for eukaryotes, but much less so prokaryotes. In particular, it still unclear whether how gram-negative bacteria acquire copper. Here, we show that Pseudomonas aeruginosa OprC outer membrane, TonB-dependent transporter conserved Proteobacteria which mediates acquisition both reduced...
Abstract We performed 2 surveys during 2022 to estimate point prevalences of SARS-CoV-2 infection compared with overall viral seroprevalence in Sweden. Point prevalence was 1.4% March and 1.5% September. Estimated >80%, including among unvaccinated children. Continued surveillance is necessary for detecting emerging, possibly more pathogenic variants.
Non-structural protein NS1 of influenza A viruses interacts with cellular factors through its N-terminal RNA-binding, middle effector and C-terminal non-structured domains. attenuates antiviral responses in infected cells thereby secures efficient virus replication. Some strains express C-terminally truncated proteins due to nonsense mutations the gene. To understand role region regulation responses, we engineered expressing using A/WSN/33(H1N1) reverse genetics tested them human macrophages...
Influenza A viruses (IAVs) impact the public health and global economy by causing yearly epidemics occasional pandemics. Several anti-IAV drugs are available many in development. However, question remains which of these antiviral agents may allow activation immune responses protect patients against co- re-infections. To answer to this question, we analysed immuno-modulating properties antivirals saliphenylhalamide (SaliPhe), SNS-032, obatoclax, gemcitabine, found that only gemcitabine did...
Sendai virus (SeV) is a common respiratory pathogen in mice, rats, and hamsters. Host cell recognition of SeV mediated by receptors, which recognize viral components induce intracellular signal transduction pathways that activate the antiviral innate immune response. Viruses use host proteins to control activities signaling their downstream targets, one most important protein modifications regulated infection phosphorylation. In this study, we used phosphoproteomics combined with...
It is possible to identify thousands of phosphopeptides and -proteins in a single experiment with mass spectrometry-based phosphoproteomics. However, current bottleneck the downstream data analysis which often laborious requires number manual steps.Toward automating steps, we have developed implemented software, PhosFox, enables peptide-level processing phosphoproteomic generated by multiple protein identification search algorithms, including Mascot, Sequest, Paragon, as well...
Human influenza A viruses (IAVs) cause global pandemics and epidemics. These evolve rapidly, making current treatment options ineffective. To identify novel modulators of IAV-host interactions, we re-analyzed our recent transcriptomics, metabolomics, proteomics, phosphoproteomics, genomics/virtual ligand screening data. We identified 713 potential targeting 200 cellular two viral proteins. Anti-influenza activity for 48 them has been reported previously, whereas the antiviral efficacy...
Viral double-stranded RNA (dsRNA) is the most important viral structure recognized by cytosolic pattern-recognition receptors of innate immune system, and its recognition results in activation signaling cascades that stimulate production antiviral cytokines apoptosis infected cells. 14-3-3 proteins are ubiquitously expressed regulatory molecules participate a variety cellular processes, protein-mediated pathways activated cytoplasmic dsRNA human keratinocytes. However, functional role...
In order to estimate the prevalence and understand spread of SARS-CoV-2 in Sweden, Public Health Agency with support from Swedish Armed Forces, conducted a series point surveys between March December 2020.Sampling material instructions on how perform self-sampling upper respiratory tract were delivered homes participants. Samples analysed by real-time PCR, participants completed questionnaires regarding symptoms.The first survey Stockholm region 2020 included 707 showed 2.5%. The following...
Abstract Research efforts to discover potential new antibiotics for Gram-negative bacteria suffer from high attrition rates due the synergistic action of efflux systems and limited permeability outer membrane (OM). One strategy overcome OM barrier is identify small molecules that are natural substrates abundant channels, use such compounds as scaffolds design efficiently-permeating antibacterials. Here we present a multidisciplinary approach small-molecule scaffolds. Focusing on pathogenic...
We illustrate the potential for specialist laboratory networks to be used as preparedness and response tool through rapid collection sharing of data. Here, Emerging Viral Diseases-Expert Laboratory Network (EVD-LabNet) a assessment chikungunya virus (CHIKV) in returning European travellers related an ongoing outbreak Thailand was this purpose. EVD-LabNet rapidly collected data on requests, diagnosed CHIKV imported cases sequences generated, shared among its members with Centre Disease...