A5028636061- Salmonella and Campylobacter epidemiology
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Trace Elements in Health
- T-cell and B-cell Immunology
- Cellular transport and secretion
- Viral gastroenteritis research and epidemiology
- Rabies epidemiology and control
- Cancer Treatment and Pharmacology
- Child Nutrition and Water Access
- Antibiotic Use and Resistance
- Microbial Metabolic Engineering and Bioproduction
- Pharmaceutical Economics and Policy
- Toxoplasma gondii Research Studies
- Chemokine receptors and signaling
- Vitamin C and Antioxidants Research
- Herpesvirus Infections and Treatments
- Vibrio bacteria research studies
- Toxin Mechanisms and Immunotoxins
- RNA Interference and Gene Delivery
- Neutropenia and Cancer Infections
- Autophagy in Disease and Therapy
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- Intraperitoneal and Appendiceal Malignancies
University of Basel
2012-2025
Pathogen access to host nutrients in infected tissues is fundamental for pathogen growth and virulence, disease progression, infection control. However, our understanding of this crucial process still rather limited because experimental conceptual challenges. Here, we used proteomics, microbial genetics, competitive infections, computational approaches obtain a comprehensive overview Salmonella nutrition mouse typhoid fever model. The data revealed that accessed an unexpectedly diverse set...
Gram-negative bacterial pathogens have an outer membrane that restricts entry of molecules into the cell. Water-filled protein channels in membrane, so-called porins, facilitate nutrient uptake and are thought to enable antibiotic entry. Here, we determined role porins a major pathogen, Pseudomonas aeruginosa, by constructing strain lacking all 40 identifiable 15 strains carrying only single unique type porin characterizing these with NMR metabolomics antimicrobial susceptibility assays. In...
Antimicrobial compounds are essential for controlling bacterial infections. Stress-induced tolerance and persisters can undermine antimicrobial activities under laboratory conditions, but their quantitative effects physiological conditions remain unclear1,2. Here we determined constraints on clearance of Salmonella by antimicrobials in infected mice tissue-mimicking chemostats. The antibiotics enrofloxacin ceftriaxone exhibited poor anti-Salmonella activity both primarily owing to severe...
Invasive Salmonella infection is an important health problem that worsening because of rising antimicrobial resistance and changing serovar spectrum. Novel vaccines with broad coverage are needed, but suitable protective antigens remain largely unknown. Here, we tested 37 broadly conserved in a mouse typhoid fever model, identified antigen candidates conferred partial protection against lethal disease. Antigen properties such as high vivo abundance or immunodominance convalescent individuals...
Antimicrobial chemotherapy can fail to eradicate the pathogen, even in absence of antimicrobial resistance. Persisting pathogens subsequently cause relapsing diseases. In vitro studies suggest various mechanisms antibiotic persistence, but their vivo relevance remains unclear because difficulty studying scarce pathogen survivors complex host tissues. Here, we localized and characterized rare surviving Salmonella mouse spleen using high-resolution whole-organ tomography. Chemotherapy cleared...
Trimeric chaperone Skp activates by an unprecedented mechanism, with folding from a disordered state coupled to trimerization.
Solute carrier family 11 member 1 (SLC11A1) is critical for host resistance to diverse intracellular pathogens. During infection, SLC11A1 limits Salmonella's access iron, zinc, and magnesium, but only magnesium deprivation significantly impairs Salmonella replication. To understand the unexpected minor impact of we determined iron in infected SLC11A1-deficient normal mice. Using reporter strains mass spectrometry purified from spleen, found that caused growth-restricting a subset Salmonella....
Abstract Virulence and persistence of the obligate intracellular parasite Toxoplasma gondii involve secretion effector proteins belonging to family dense granule (GRAs) that act notably as modulators host defense mechanisms participate in cyst wall formation. The subset GRAs residing parasitophorous vacuole (PV) or exported into cell, undergo proteolytic cleavage Golgi upon action aspartyl protease 5 (ASP5). In tachyzoites, ASP5 substrates play central roles morphology PV export effectors...
Introduction: Blood infections from multi-drug-resistant Salmonella pose a major health burden. This is especially true because can survive and replicate intracellularly, the development of new treatment strategies dependent on expensive time-consuming in vivo trials. The aim this study was to develop Salmonella-infection model that makes it possible directly observe macrophages use test effect antimicrobials against intra- extracellular order close gap between vitro rodent-infection models....
Abstract Iron controls bacterial infections through diverse pathogen and host mechanisms that remain challenging to disentangle. Here, we determined how individual Salmonella cells access iron in infected mice. Our results showed the transporter SLC11A1 restricted availability. However, many bypassed this restriction by targeting macrophage endosomes contained remnants of iron-rich red blood cells. These iron-replete bacteria dominated overall growth masked relieve iron-starved under...