A5011526532- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- Escherichia coli research studies
- Bacterial biofilms and quorum sensing
- Hormonal and reproductive studies
- Immunotherapy and Immune Responses
- Salmonella and Campylobacter epidemiology
- Immune cells in cancer
- Exercise and Physiological Responses
- Antibiotic Resistance in Bacteria
- Polysaccharides and Plant Cell Walls
- Lipid Membrane Structure and Behavior
- Ubiquitin and proteasome pathways
- Invertebrate Immune Response Mechanisms
- Intraperitoneal and Appendiceal Malignancies
- Signaling Pathways in Disease
- Hypothalamic control of reproductive hormones
- Toxin Mechanisms and Immunotoxins
- Phagocytosis and Immune Regulation
- Neutropenia and Cancer Infections
- Evolution and Genetic Dynamics
- Advanced Glycation End Products research
- Cancer Treatment and Pharmacology
Newcastle University
2021-2022
University of Basel
2019-2021
University of Dundee
2013-2018
University of Florence
1990
The Type VI secretion system (T6SS) is widespread among bacterial pathogens and acts as an effective weapon against competitor bacteria eukaryotic hosts by delivering toxic effector proteins directly into target cells. T6SS utilises a bacteriophage-like contractile machinery to expel puncturing device based on tube of Hcp topped with VgrG spike, which can be extended final tip from PAAR domain-containing protein. Effector are believed delivered specifically associating particular Hcp, or...
In response to infection by fungal pathogens, the innate immune system recognises specific pathogen associated molecular patterns (PAMPs) via pattern recognition receptors including C-type lectin dectin-1 and members of Toll Like Receptor (TLR) family. Stimulation these leads induction both pro- anti-inflammatory cytokines. The protein kinases MSK1 2 are known be important in limiting inflammatory cytokine production macrophages TLR4 agonist LPS. this study we show that MSKs also activated...
Biofilm formation by the Gram-positive bacterium Bacillus subtilis is tightly controlled at level of transcription. The biofilm contains specialized cell types that arise from differentiation resident isogenic bacteria. DegU a response regulator controls several social behaviours exhibited B. including swarming motility, and extracellular protease (exoprotease) production. Here, for first time, we examine prevalence origin exoprotease-producing cells within biofilm. This was accomplished...
Gram-negative bacteria use the Type VI secretion system (T6SS) to inject toxic proteins into rival or eukaryotic cells. However, mechanism of T6SS is incompletely understood. In present study, we investigated a conserved component T6SS, TssK, using antibacterial Serratia marcescens as model system. TssK was confirmed be essential for effector by T6SS. The native protein, although not an integral membrane appeared localize inner membrane, consistent with its presence within membrane-anchored...
The Type VI secretion system (T6SS) is widely used by bacterial pathogens as an effective weapon against competitors and also deployed host eukaryotic cells in some cases. It a contractile nanomachine which delivers toxic effector proteins directly into target dynamic cycles of assembly firing. Bacterial adopt distinct post-translational regulatory strategies for deployment the T6SS. ‘Defensive’ T6SSs assemble fire response to incoming attacks from aggressive neighbouring cells, can utilise...
Gene editing is key for elucidating gene function. Traditional methods, such as consecutive single-crossovers, have been widely used to modify bacterial genomes. However, cumbersome cloning and limited efficiency of negative selection often make this method slower than other methods recombineering.Here, we established a time-effective variant single-crossovers. This exploits rapid plasmid construction using Gibson assembly, convenient E. coli donor strain, efficient dual-negative improved...
Antimicrobial chemotherapy can fail to eradicate the pathogen, even in absence of antimicrobial resistance. Persisting pathogens subsequently cause relapsing diseases. In vitro studies suggest various mechanisms antibiotic persistence, but their vivo relevance remains unclear because difficulty studying scarce pathogen survivors complex host tissues. Here, we localized and characterized rare surviving Salmonella mouse spleen using high-resolution whole-organ tomography. Chemotherapy cleared...
Abstract Phagocytosis is a key process in innate immunity and homeostasis. After particle uptake, newly formed phagosomes mature by acquisition of endolysosomal enzymes. Macrophage activation interferon gamma (IFN‐γ) increases microbicidal activity, but delays phagosomal maturation an unknown mechanism. Using quantitative proteomics, we show that proteins harbour high levels typical atypical ubiquitin chain types. Moreover, ubiquitylation vesicle trafficking substantially enhanced upon IFN‐γ...
Macrophages engulf pathogens into dynamic phagosomes, which many bacteria manipulate for survival. However, isolating pure pathogen-containing phagosomes remains challenging. Here, we developed a novel flow cytometry-based isolation and ultrasensitive proteomics approach to analyse phagosomal bacterial proteomes from macrophages infected with wild-type (WT) Salmonella enterica serovar Typhimurium (STM), ΔphoP mutant, or dead WT at 30 min 4 hrs post-infection. Our provides higher throughput,...
Abstract We describe a versatile method for chromosomal gene editing based on classical consecutive single-crossovers. The exploits rapid plasmid construction using Gibson assembly, convenient E. coli donor strain, and efficient dual-negative selection improved suicide vector resolution. used this to generate in frame deletions, insertions point mutations Salmonella enterica with limited hands-on time. Similar strategies allowed also Pseudomonas aeruginosa multi-drug-resistant (MDR)...
Summary Phagocytosis is a key process in innate immunity and homeostasis. After uptake, newly formed phagosomes mature by acquisition of endo-lysosomal enzymes. Macrophage activation interferon-gamma (IFN-γ) increases microbicidal activity, but delays phagosomal maturation an unknown mechanism. Using quantitative proteomics, we show that proteins harbour high levels typical atypical ubiquitin chain types. Moreover, ubiquitylation vesicle trafficking substantially enhanced upon IFN-γ...
Enteric fever is major health issue in developing countries and it becoming progressively untreatable due to increase of antimicrobial resistance. The causative agent, Salmonella enterica , replicates host phagocytes various organs regulates expression hundreds genes response signals. PhoPQ one the key regulators essential for virulence humans a mouse typhoid model. sensor kinase PhoQ responds diverse stimuli (Mg2+ limitation, low pH, cationic peptides, high osmolarity, and, indirectly,...